Schlumbergera virus X
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6UNI9|Q6UNI9_9VIRU Triple gene block protein 2 OS=Schlumbergera virus X OX=253700 PE=4 SV=1
MM1 pKa = 7.33 FLLYY5 pKa = 10.7 SLIGVVVGIAIIVVLNTLNSPSCTIILNGHH35 pKa = 4.78 STIIQGCTQLSNLDD49 pKa = 3.83 SVIEE53 pKa = 4.01 ALNHH57 pKa = 5.79 RR58 pKa = 11.84 LSS60 pKa = 3.5
Molecular weight: 6.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.57
IPC2_protein 5.728
IPC_protein 5.677
Toseland 6.097
ProMoST 6.008
Dawson 6.008
Bjellqvist 5.944
Wikipedia 6.046
Rodwell 6.008
Grimsley 6.211
Solomon 6.008
Lehninger 6.008
Nozaki 6.275
DTASelect 6.478
Thurlkill 6.504
EMBOSS 6.478
Sillero 6.402
Patrickios 0.604
IPC_peptide 6.033
IPC2_peptide 6.427
IPC2.peptide.svr19 6.364
Protein with the highest isoelectric point:
>tr|Q6UNJ0|Q6UNJ0_9VIRU Triple gene block protein 1 OS=Schlumbergera virus X OX=253700 PE=4 SV=1
MM1 pKa = 7.4 SGAPIHH7 pKa = 6.13 LTPPPDD13 pKa = 3.26 QTKK16 pKa = 10.61 VIFPLTVGLGLTLVVYY32 pKa = 10.64 SLTRR36 pKa = 11.84 STLPHH41 pKa = 6.78 VGDD44 pKa = 4.02 STHH47 pKa = 6.49 SLPHH51 pKa = 6.23 GGTYY55 pKa = 9.76 RR56 pKa = 11.84 DD57 pKa = 3.57 GTKK60 pKa = 10.22 SVNYY64 pKa = 7.17 YY65 pKa = 10.2 QPSHH69 pKa = 6.62 PVSNHH74 pKa = 5.52 LPLIFVLGLIVAIYY88 pKa = 10.7 VSTLFSNRR96 pKa = 11.84 SRR98 pKa = 11.84 GRR100 pKa = 11.84 HH101 pKa = 5.62 CNHH104 pKa = 6.97 CGAKK108 pKa = 9.82 HH109 pKa = 5.11 AA110 pKa = 4.94
Molecular weight: 11.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.829
IPC2_protein 9.268
IPC_protein 9.443
Toseland 9.399
ProMoST 9.399
Dawson 9.765
Bjellqvist 9.619
Wikipedia 10.014
Rodwell 9.853
Grimsley 9.882
Solomon 9.867
Lehninger 9.809
Nozaki 9.589
DTASelect 9.575
Thurlkill 9.589
EMBOSS 9.853
Sillero 9.736
Patrickios 4.202
IPC_peptide 9.838
IPC2_peptide 8.756
IPC2.peptide.svr19 7.936
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2169
60
1545
433.8
48.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.1 ± 0.739
1.291 ± 0.234
4.518 ± 0.772
5.994 ± 1.201
4.472 ± 0.636
5.118 ± 0.573
3.642 ± 0.743
5.671 ± 0.795
6.132 ± 1.23
9.959 ± 1.193
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.982 ± 0.45
4.518 ± 0.639
6.455 ± 0.512
4.61 ± 0.359
4.195 ± 0.258
7.423 ± 1.252
7.146 ± 0.386
5.486 ± 0.88
1.199 ± 0.314
3.089 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here