Peach chlorotic mottle virus
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8C5N4|A8C5N4_9VIRU Movement protein TGB2 OS=Peach chlorotic mottle virus OX=471498 PE=3 SV=1
MM1 pKa = 7.45 DD2 pKa = 4.17 TVVSLLFEE10 pKa = 4.47 YY11 pKa = 10.64 GFEE14 pKa = 4.1 RR15 pKa = 11.84 TSVPIEE21 pKa = 4.41 DD22 pKa = 4.06 KK23 pKa = 11.11 LIVHH27 pKa = 6.95 AVPGSGKK34 pKa = 6.97 TTLIRR39 pKa = 11.84 EE40 pKa = 4.29 ALNRR44 pKa = 11.84 NLGIEE49 pKa = 4.06 AFSFGEE55 pKa = 3.97 PDD57 pKa = 4.78 LPNIWGRR64 pKa = 11.84 YY65 pKa = 5.41 IKK67 pKa = 10.43 KK68 pKa = 10.31 AISGQKK74 pKa = 8.72 GTGSFCILDD83 pKa = 3.88 EE84 pKa = 4.22 YY85 pKa = 11.42 LSGEE89 pKa = 4.18 FGTGFDD95 pKa = 4.72 CFFSDD100 pKa = 3.37 PHH102 pKa = 6.98 QNSGDD107 pKa = 3.73 CAPAHH112 pKa = 5.08 FVGRR116 pKa = 11.84 SSQRR120 pKa = 11.84 FGRR123 pKa = 11.84 NTAGLLQSLGYY134 pKa = 9.73 SVNSAKK140 pKa = 10.49 DD141 pKa = 3.35 DD142 pKa = 3.71 EE143 pKa = 5.75 LIFEE147 pKa = 4.38 NVFRR151 pKa = 11.84 AEE153 pKa = 4.03 LEE155 pKa = 4.12 GAVICVEE162 pKa = 4.1 KK163 pKa = 10.88 NVEE166 pKa = 5.07 DD167 pKa = 4.19 FLRR170 pKa = 11.84 WNHH173 pKa = 5.86 CEE175 pKa = 3.75 YY176 pKa = 10.9 RR177 pKa = 11.84 LPCQVRR183 pKa = 11.84 GSTFEE188 pKa = 4.01 VVTFIHH194 pKa = 6.61 EE195 pKa = 4.31 LPLDD199 pKa = 3.73 QLVGPDD205 pKa = 4.16 LYY207 pKa = 10.63 IALTRR212 pKa = 11.84 HH213 pKa = 5.22 SKK215 pKa = 10.44 KK216 pKa = 10.27 IQILTNN222 pKa = 3.61
Molecular weight: 24.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.108
IPC2_protein 5.181
IPC_protein 5.118
Toseland 5.13
ProMoST 5.296
Dawson 5.156
Bjellqvist 5.258
Wikipedia 5.029
Rodwell 5.08
Grimsley 5.08
Solomon 5.156
Lehninger 5.118
Nozaki 5.296
DTASelect 5.436
Thurlkill 5.169
EMBOSS 5.105
Sillero 5.372
Patrickios 3.897
IPC_peptide 5.156
IPC2_peptide 5.372
IPC2.peptide.svr19 5.328
Protein with the highest isoelectric point:
>tr|A8C5N5|A8C5N5_9VIRU Movement protein TGBp3 OS=Peach chlorotic mottle virus OX=471498 PE=3 SV=1
MM1 pKa = 7.88 PLAQPPDD8 pKa = 3.72 FSKK11 pKa = 11.13 SVFPLAIGLAVGIVIFALTRR31 pKa = 11.84 STLPHH36 pKa = 6.55 AGDD39 pKa = 4.63 NIHH42 pKa = 6.71 HH43 pKa = 6.9 LPHH46 pKa = 6.55 GGSYY50 pKa = 11.12 VDD52 pKa = 3.52 GTKK55 pKa = 10.3 KK56 pKa = 10.29 INYY59 pKa = 7.51 CGPKK63 pKa = 10.03 EE64 pKa = 4.16 SFPTPGVLGLKK75 pKa = 10.13 FYY77 pKa = 10.89 AFCLACCILAYY88 pKa = 10.38 LHH90 pKa = 6.15 ATFRR94 pKa = 11.84 GNNSSVRR101 pKa = 11.84 CPTCINNPQHH111 pKa = 6.56 CVRR114 pKa = 11.84 SS115 pKa = 3.84
Molecular weight: 12.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.929
IPC2_protein 7.951
IPC_protein 7.863
Toseland 7.395
ProMoST 8.346
Dawson 8.478
Bjellqvist 8.946
Wikipedia 8.39
Rodwell 8.492
Grimsley 7.38
Solomon 8.624
Lehninger 8.639
Nozaki 9.238
DTASelect 8.595
Thurlkill 8.653
EMBOSS 8.697
Sillero 9.019
Patrickios 1.202
IPC_peptide 8.609
IPC2_peptide 8.404
IPC2.peptide.svr19 8.324
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2941
67
2135
588.2
65.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.916 ± 1.451
2.822 ± 0.717
4.998 ± 0.78
5.848 ± 0.713
6.358 ± 0.684
6.698 ± 0.987
2.618 ± 0.724
6.256 ± 0.582
6.12 ± 1.151
9.895 ± 0.845
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.87 ± 0.437
4.25 ± 0.272
4.76 ± 0.963
2.822 ± 0.296
4.726 ± 0.42
9.351 ± 0.994
5.168 ± 1.719
5.78 ± 0.634
1.054 ± 0.261
2.686 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here