Wenling frogfish filovirus
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1GML8|A0A2P1GML8_9MONO Nucleoprotein OS=Wenling frogfish filovirus OX=2116487 PE=3 SV=1
MM1 pKa = 8.07 DD2 pKa = 5.25 MDD4 pKa = 4.91 TMDD7 pKa = 4.9 LSCIAGSNTGGEE19 pKa = 4.26 NPFQRR24 pKa = 11.84 LDD26 pKa = 3.7 GLPALQLGTISRR38 pKa = 11.84 VRR40 pKa = 11.84 DD41 pKa = 3.47 LEE43 pKa = 4.08 ATMEE47 pKa = 4.31 TPQTQPQPQATTRR60 pKa = 11.84 QNLADD65 pKa = 4.34 DD66 pKa = 4.89 VYY68 pKa = 10.87 AQLGAEE74 pKa = 4.39 TKK76 pKa = 10.52 EE77 pKa = 4.37 EE78 pKa = 4.47 PDD80 pKa = 3.38 DD81 pKa = 3.87 QYY83 pKa = 11.96 EE84 pKa = 4.05 GDD86 pKa = 4.04 EE87 pKa = 4.31 PDD89 pKa = 4.95 DD90 pKa = 3.68 YY91 pKa = 11.5 FSAIEE96 pKa = 4.34 TIVGSCMKK104 pKa = 10.69 GFAEE108 pKa = 4.67 QLNQQGQHH116 pKa = 6.76 LMILQKK122 pKa = 10.94 KK123 pKa = 9.43 LDD125 pKa = 3.87 LVQVGVAEE133 pKa = 4.52 IEE135 pKa = 3.98 QSLRR139 pKa = 11.84 ASTVTLADD147 pKa = 3.61 EE148 pKa = 5.43 LKK150 pKa = 9.87 TDD152 pKa = 3.45 MAVLVKK158 pKa = 10.05 TISQVSAEE166 pKa = 4.53 LKK168 pKa = 10.54 NLVTITGPAAGTADD182 pKa = 3.16 AVVRR186 pKa = 11.84 CLSQGRR192 pKa = 11.84 QLPDD196 pKa = 3.36 VPCVISEE203 pKa = 4.3 SSVPTIQHH211 pKa = 6.46 LVATAPPTRR220 pKa = 11.84 SAVDD224 pKa = 3.72 LGAALDD230 pKa = 3.79 MYY232 pKa = 9.83 QTVEE236 pKa = 4.13 EE237 pKa = 4.23 EE238 pKa = 3.74 QGEE241 pKa = 4.49 NFGRR245 pKa = 11.84 PVYY248 pKa = 10.59 EE249 pKa = 4.06 GDD251 pKa = 3.57 EE252 pKa = 4.16 LAEE255 pKa = 5.33 DD256 pKa = 3.66 ICSLITTRR264 pKa = 11.84 NPALFKK270 pKa = 10.54 KK271 pKa = 10.6 IKK273 pKa = 10.56 AEE275 pKa = 3.45 VDD277 pKa = 3.09 AAGEE281 pKa = 4.18 EE282 pKa = 4.28 QASRR286 pKa = 11.84 IYY288 pKa = 10.75 TEE290 pKa = 5.08 LLPMDD295 pKa = 3.77 NTSRR299 pKa = 11.84 EE300 pKa = 3.88 AYY302 pKa = 8.69 IFQWAKK308 pKa = 8.8 IQRR311 pKa = 11.84 TEE313 pKa = 3.92 KK314 pKa = 10.35 HH315 pKa = 6.26 HH316 pKa = 6.63 LPHH319 pKa = 6.97 PASGNHH325 pKa = 4.6 QVTKK329 pKa = 10.39 KK330 pKa = 9.7 VVVMKK335 pKa = 8.84 TPPGPEE341 pKa = 3.6 YY342 pKa = 10.59 MLMNRR347 pKa = 11.84 KK348 pKa = 6.75 PTKK351 pKa = 9.91 RR352 pKa = 11.84 DD353 pKa = 3.09 LQEE356 pKa = 3.34 FDD358 pKa = 5.36 KK359 pKa = 10.85 IVKK362 pKa = 10.14 YY363 pKa = 10.58 DD364 pKa = 3.4 GQYY367 pKa = 11.08 FSTT370 pKa = 4.36
Molecular weight: 40.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.664
IPC2_protein 4.635
IPC_protein 4.571
Toseland 4.431
ProMoST 4.685
Dawson 4.52
Bjellqvist 4.673
Wikipedia 4.393
Rodwell 4.418
Grimsley 4.342
Solomon 4.52
Lehninger 4.482
Nozaki 4.635
DTASelect 4.8
Thurlkill 4.431
EMBOSS 4.418
Sillero 4.698
Patrickios 3.935
IPC_peptide 4.533
IPC2_peptide 4.685
IPC2.peptide.svr19 4.651
Protein with the highest isoelectric point:
>tr|A0A2P1GML3|A0A2P1GML3_9MONO Putative glycoprotein 2 OS=Wenling frogfish filovirus OX=2116487 PE=4 SV=1
MM1 pKa = 7.01 TASNPAAIALICMGCFLGLVLTWGLADD28 pKa = 5.31 LICRR32 pKa = 11.84 CKK34 pKa = 10.51 RR35 pKa = 11.84 RR36 pKa = 11.84 AQKK39 pKa = 10.32 QKK41 pKa = 10.68 ARR43 pKa = 11.84 RR44 pKa = 11.84 ALPRR48 pKa = 11.84 LRR50 pKa = 11.84 IVSDD54 pKa = 3.83 SSALTLQTDD63 pKa = 3.83 VTSGLSSPNGSRR75 pKa = 11.84 PISRR79 pKa = 11.84 FSDD82 pKa = 4.0 CNLHH86 pKa = 6.99 HH87 pKa = 7.21 PWAQEE92 pKa = 3.75 KK93 pKa = 10.31 LLHH96 pKa = 5.72 IVLRR100 pKa = 11.84 EE101 pKa = 3.94 TTPLGDD107 pKa = 3.19 IKK109 pKa = 10.86 LVPFLRR115 pKa = 11.84 LSHH118 pKa = 6.24 HH119 pKa = 5.93 QFSEE123 pKa = 3.74 DD124 pKa = 3.33 MKK126 pKa = 11.0 IMRR129 pKa = 11.84 SNLYY133 pKa = 10.74 GFVGWLRR140 pKa = 11.84 AEE142 pKa = 4.0 CLIRR146 pKa = 11.84 RR147 pKa = 11.84 GSQQHH152 pKa = 5.6 PVWSVVQTLNSPDD165 pKa = 3.66 HH166 pKa = 6.42 RR167 pKa = 11.84 WAGSRR172 pKa = 11.84 LKK174 pKa = 11.34 GEE176 pKa = 4.38 GFPLGKK182 pKa = 9.67 ISQAAVLAVLDD193 pKa = 4.2 VVVHH197 pKa = 6.49 GSHH200 pKa = 6.64 PHH202 pKa = 5.72 LGYY205 pKa = 11.19 ALRR208 pKa = 11.84 HH209 pKa = 6.26 HH210 pKa = 6.86 IADD213 pKa = 4.16 TYY215 pKa = 10.93 RR216 pKa = 11.84 PGVRR220 pKa = 11.84 PRR222 pKa = 11.84 TALII226 pKa = 3.81
Molecular weight: 25.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.472
IPC_protein 10.087
Toseland 10.57
ProMoST 10.233
Dawson 10.657
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.862
Grimsley 10.701
Solomon 10.774
Lehninger 10.745
Nozaki 10.599
DTASelect 10.35
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.599
Patrickios 10.599
IPC_peptide 10.774
IPC2_peptide 9.663
IPC2.peptide.svr19 8.551
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
5282
151
2116
528.2
58.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.819 ± 0.332
2.007 ± 0.22
5.168 ± 0.409
5.566 ± 0.437
3.597 ± 0.429
6.285 ± 0.352
2.897 ± 0.315
4.979 ± 0.215
4.619 ± 0.444
10.167 ± 0.491
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.669 ± 0.267
3.351 ± 0.259
5.718 ± 0.634
4.033 ± 0.34
6.02 ± 0.452
7.762 ± 0.505
7.062 ± 0.504
6.134 ± 0.178
1.155 ± 0.181
2.991 ± 0.288
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here