Hyella patelloides LEGE 07179
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7698 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A563VS95|A0A563VS95_9CYAN Sugar transferase OS=Hyella patelloides LEGE 07179 OX=945734 GN=H1P_2540001 PE=3 SV=1
MM1 pKa = 8.0 FIGLCCNQKK10 pKa = 10.38 IIINLSVRR18 pKa = 11.84 IMLNRR23 pKa = 11.84 VFNSLSFKK31 pKa = 10.85 AIALSSFFVICHH43 pKa = 5.09 VGEE46 pKa = 4.54 AKK48 pKa = 10.39 AQNTLEE54 pKa = 4.29 VPSTDD59 pKa = 3.76 YY60 pKa = 10.73 QDD62 pKa = 4.94 EE63 pKa = 4.4 MGQVTNVNQLRR74 pKa = 11.84 DD75 pKa = 3.68 VSPADD80 pKa = 3.24 WSYY83 pKa = 10.69 EE84 pKa = 3.83 ALRR87 pKa = 11.84 SLVDD91 pKa = 3.76 RR92 pKa = 11.84 YY93 pKa = 11.19 GCIAGFPNQAYY104 pKa = 9.82 RR105 pKa = 11.84 GSQALSRR112 pKa = 11.84 YY113 pKa = 8.62 EE114 pKa = 3.76 FAAGLNSCLNQIEE127 pKa = 4.27 RR128 pKa = 11.84 LIASSEE134 pKa = 4.0 AVVRR138 pKa = 11.84 EE139 pKa = 4.69 DD140 pKa = 3.03 IDD142 pKa = 3.92 TINRR146 pKa = 11.84 LTQEE150 pKa = 4.41 FEE152 pKa = 4.45 SEE154 pKa = 3.93 LATIGGRR161 pKa = 11.84 IDD163 pKa = 3.52 SLEE166 pKa = 3.91 SRR168 pKa = 11.84 TAFLEE173 pKa = 4.26 DD174 pKa = 3.47 NQFSTTTKK182 pKa = 10.6 LNAQVVSYY190 pKa = 11.24 VLGTFGDD197 pKa = 4.03 EE198 pKa = 4.46 RR199 pKa = 11.84 PDD201 pKa = 3.49 GTDD204 pKa = 2.76 VDD206 pKa = 4.25 DD207 pKa = 5.35 QITFSSRR214 pKa = 11.84 VRR216 pKa = 11.84 LNFDD220 pKa = 3.23 SSFTGEE226 pKa = 3.77 DD227 pKa = 3.55 FLRR230 pKa = 11.84 VRR232 pKa = 11.84 IQARR236 pKa = 11.84 NITSPAEE243 pKa = 4.08 SGGTNSLALNMEE255 pKa = 4.44 GDD257 pKa = 3.8 SEE259 pKa = 4.99 NNVEE263 pKa = 5.91 LDD265 pKa = 3.18 DD266 pKa = 4.83 LYY268 pKa = 11.52 YY269 pKa = 10.16 IFPVTEE275 pKa = 3.87 QVTATVGTNGVDD287 pKa = 2.95 VDD289 pKa = 4.72 EE290 pKa = 5.49 IFDD293 pKa = 3.76 VVPTMGIAYY302 pKa = 8.05 DD303 pKa = 3.77 ALSLYY308 pKa = 10.37 SAYY311 pKa = 11.26 NNLIYY316 pKa = 11.03 SNGNGGGAGIGASLEE331 pKa = 4.11 FVEE334 pKa = 5.35 WADD337 pKa = 3.9 LQIGYY342 pKa = 7.93 WAPNPADD349 pKa = 3.98 PDD351 pKa = 3.63 EE352 pKa = 4.91 GNGLFNGNYY361 pKa = 7.73 TAGANLNLSFLDD373 pKa = 3.28 EE374 pKa = 4.39 RR375 pKa = 11.84 LNVAVAYY382 pKa = 10.53 LRR384 pKa = 11.84 GYY386 pKa = 9.21 QGQGSGYY393 pKa = 10.23 DD394 pKa = 3.18 LGGFTGTDD402 pKa = 3.45 GATDD406 pKa = 3.97 PFDD409 pKa = 4.55 DD410 pKa = 5.03 QANSSNNFGVATSFQVFEE428 pKa = 4.01 QLTVGGYY435 pKa = 9.89 FGYY438 pKa = 9.05 ATASLVGDD446 pKa = 4.78 DD447 pKa = 4.87 DD448 pKa = 4.33 TNADD452 pKa = 3.34 ILTWNAYY459 pKa = 7.73 LTYY462 pKa = 10.46 TDD464 pKa = 4.47 LLKK467 pKa = 10.94 EE468 pKa = 4.15 GSTALVSFGQPPSLIDD484 pKa = 3.69 SEE486 pKa = 4.56 GDD488 pKa = 3.26 ALADD492 pKa = 4.86 DD493 pKa = 4.8 EE494 pKa = 4.88 DD495 pKa = 4.66 TPFLLNVEE503 pKa = 4.25 YY504 pKa = 10.4 QYY506 pKa = 11.72 QLNDD510 pKa = 4.94 FIQLTPGAYY519 pKa = 10.3 VLFNPNGNSDD529 pKa = 3.32 NDD531 pKa = 4.2 TIYY534 pKa = 10.89 VGTVRR539 pKa = 11.84 TIFSFF544 pKa = 3.57
Molecular weight: 59.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.783
IPC_protein 3.821
Toseland 3.592
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.63
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.935
Patrickios 0.96
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.819
Protein with the highest isoelectric point:
>tr|A0A563VKW9|A0A563VKW9_9CYAN Toxin of the ChpA-ChpR toxin-antitoxin system endoribonuclease OS=Hyella patelloides LEGE 07179 OX=945734 GN=chpA PE=3 SV=1
MM1 pKa = 7.55 TKK3 pKa = 9.13 RR4 pKa = 11.84 TLGGTNRR11 pKa = 11.84 KK12 pKa = 7.61 QKK14 pKa = 8.99 RR15 pKa = 11.84 TSGFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 SHH26 pKa = 6.52 TGRR29 pKa = 11.84 RR30 pKa = 11.84 VIRR33 pKa = 11.84 SRR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.21 KK38 pKa = 9.62 GRR40 pKa = 11.84 HH41 pKa = 4.8 RR42 pKa = 11.84 LAVV45 pKa = 3.37
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.735
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.457
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7698
0
7698
2095954
20
4713
272.3
30.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.294 ± 0.026
1.015 ± 0.01
5.135 ± 0.032
6.54 ± 0.033
4.126 ± 0.023
6.235 ± 0.039
1.712 ± 0.015
7.543 ± 0.024
5.607 ± 0.037
10.782 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.778 ± 0.011
5.083 ± 0.028
4.095 ± 0.021
5.124 ± 0.032
4.663 ± 0.023
6.827 ± 0.023
5.751 ± 0.028
6.079 ± 0.02
1.366 ± 0.013
3.245 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here