Amino acid dipepetide frequency for Mycobacterium phage Royals2015

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.749AlaAla: 12.749 ± 1.443
1.302AlaCys: 1.302 ± 0.29
7.541AlaAsp: 7.541 ± 0.617
6.619AlaGlu: 6.619 ± 0.639
2.821AlaPhe: 2.821 ± 0.413
9.44AlaGly: 9.44 ± 1.156
2.441AlaHis: 2.441 ± 0.411
3.96AlaIle: 3.96 ± 0.504
4.232AlaLys: 4.232 ± 0.439
7.866AlaLeu: 7.866 ± 0.776
2.875AlaMet: 2.875 ± 0.464
2.767AlaAsn: 2.767 ± 0.425
4.991AlaPro: 4.991 ± 0.589
3.798AlaGln: 3.798 ± 0.428
7.812AlaArg: 7.812 ± 0.694
5.317AlaSer: 5.317 ± 0.53
6.564AlaThr: 6.564 ± 0.571
6.564AlaVal: 6.564 ± 0.569
2.604AlaTrp: 2.604 ± 0.369
2.17AlaTyr: 2.17 ± 0.349
0.0AlaXaa: 0.0 ± 0.0
Cys
1.031CysAla: 1.031 ± 0.334
0.217CysCys: 0.217 ± 0.111
0.977CysAsp: 0.977 ± 0.222
0.922CysGlu: 0.922 ± 0.271
0.271CysPhe: 0.271 ± 0.108
2.17CysGly: 2.17 ± 0.518
0.326CysHis: 0.326 ± 0.137
0.271CysIle: 0.271 ± 0.159
0.38CysLys: 0.38 ± 0.127
0.76CysLeu: 0.76 ± 0.257
0.326CysMet: 0.326 ± 0.143
0.488CysAsn: 0.488 ± 0.183
0.977CysPro: 0.977 ± 0.237
0.326CysGln: 0.326 ± 0.124
1.194CysArg: 1.194 ± 0.304
0.977CysSer: 0.977 ± 0.265
1.031CysThr: 1.031 ± 0.302
0.76CysVal: 0.76 ± 0.178
0.326CysTrp: 0.326 ± 0.121
0.163CysTyr: 0.163 ± 0.087
0.0CysXaa: 0.0 ± 0.0
Asp
6.293AspAla: 6.293 ± 0.586
0.922AspCys: 0.922 ± 0.266
4.232AspAsp: 4.232 ± 0.606
3.635AspGlu: 3.635 ± 0.486
1.682AspPhe: 1.682 ± 0.244
6.619AspGly: 6.619 ± 0.644
1.356AspHis: 1.356 ± 0.265
2.17AspIle: 2.17 ± 0.305
1.845AspLys: 1.845 ± 0.272
5.751AspLeu: 5.751 ± 0.522
1.194AspMet: 1.194 ± 0.286
1.573AspAsn: 1.573 ± 0.32
4.666AspPro: 4.666 ± 0.624
2.279AspGln: 2.279 ± 0.341
5.154AspArg: 5.154 ± 0.578
3.96AspSer: 3.96 ± 0.523
4.34AspThr: 4.34 ± 0.588
4.611AspVal: 4.611 ± 0.535
1.139AspTrp: 1.139 ± 0.254
2.062AspTyr: 2.062 ± 0.329
0.0AspXaa: 0.0 ± 0.0
Glu
6.239GluAla: 6.239 ± 0.678
1.085GluCys: 1.085 ± 0.28
2.658GluAsp: 2.658 ± 0.328
3.092GluGlu: 3.092 ± 0.525
2.116GluPhe: 2.116 ± 0.341
2.93GluGly: 2.93 ± 0.374
1.519GluHis: 1.519 ± 0.329
1.899GluIle: 1.899 ± 0.324
1.736GluLys: 1.736 ± 0.252
5.1GluLeu: 5.1 ± 0.642
1.465GluMet: 1.465 ± 0.274
1.519GluAsn: 1.519 ± 0.28
2.984GluPro: 2.984 ± 0.41
2.713GluGln: 2.713 ± 0.421
5.154GluArg: 5.154 ± 0.569
2.713GluSer: 2.713 ± 0.442
4.015GluThr: 4.015 ± 0.509
4.286GluVal: 4.286 ± 0.605
1.628GluTrp: 1.628 ± 0.303
1.628GluTyr: 1.628 ± 0.402
0.0GluXaa: 0.0 ± 0.0
Phe
2.55PheAla: 2.55 ± 0.379
0.163PheCys: 0.163 ± 0.09
2.713PheAsp: 2.713 ± 0.444
1.736PheGlu: 1.736 ± 0.256
1.031PhePhe: 1.031 ± 0.251
2.93PheGly: 2.93 ± 0.683
0.488PheHis: 0.488 ± 0.17
1.519PheIle: 1.519 ± 0.331
0.814PheLys: 0.814 ± 0.211
2.007PheLeu: 2.007 ± 0.279
0.705PheMet: 0.705 ± 0.194
1.194PheAsn: 1.194 ± 0.35
1.628PhePro: 1.628 ± 0.244
0.977PheGln: 0.977 ± 0.248
1.248PheArg: 1.248 ± 0.217
1.573PheSer: 1.573 ± 0.274
2.496PheThr: 2.496 ± 0.38
1.953PheVal: 1.953 ± 0.311
0.488PheTrp: 0.488 ± 0.181
0.868PheTyr: 0.868 ± 0.272
0.0PheXaa: 0.0 ± 0.0
Gly
9.819GlyAla: 9.819 ± 1.106
1.519GlyCys: 1.519 ± 0.359
5.534GlyAsp: 5.534 ± 0.475
3.852GlyGlu: 3.852 ± 0.453
2.821GlyPhe: 2.821 ± 0.361
10.796GlyGly: 10.796 ± 1.968
1.628GlyHis: 1.628 ± 0.266
4.286GlyIle: 4.286 ± 0.624
2.441GlyLys: 2.441 ± 0.369
5.751GlyLeu: 5.751 ± 0.534
2.713GlyMet: 2.713 ± 0.482
3.092GlyAsn: 3.092 ± 0.355
3.906GlyPro: 3.906 ± 0.551
2.441GlyGln: 2.441 ± 0.545
5.534GlyArg: 5.534 ± 0.658
6.727GlySer: 6.727 ± 0.904
6.076GlyThr: 6.076 ± 0.736
5.317GlyVal: 5.317 ± 0.552
2.93GlyTrp: 2.93 ± 0.474
1.899GlyTyr: 1.899 ± 0.387
0.0GlyXaa: 0.0 ± 0.0
His
2.007HisAla: 2.007 ± 0.376
0.326HisCys: 0.326 ± 0.181
1.248HisAsp: 1.248 ± 0.232
0.977HisGlu: 0.977 ± 0.283
0.76HisPhe: 0.76 ± 0.169
1.736HisGly: 1.736 ± 0.327
0.868HisHis: 0.868 ± 0.254
1.411HisIle: 1.411 ± 0.355
0.977HisLys: 0.977 ± 0.281
1.139HisLeu: 1.139 ± 0.264
0.488HisMet: 0.488 ± 0.138
0.868HisAsn: 0.868 ± 0.209
1.519HisPro: 1.519 ± 0.256
0.814HisGln: 0.814 ± 0.233
2.279HisArg: 2.279 ± 0.399
0.868HisSer: 0.868 ± 0.22
1.899HisThr: 1.899 ± 0.413
1.519HisVal: 1.519 ± 0.317
0.488HisTrp: 0.488 ± 0.157
0.868HisTyr: 0.868 ± 0.198
0.0HisXaa: 0.0 ± 0.0
Ile
5.479IleAla: 5.479 ± 0.535
0.543IleCys: 0.543 ± 0.203
4.069IleAsp: 4.069 ± 0.476
3.418IleGlu: 3.418 ± 0.409
0.597IlePhe: 0.597 ± 0.207
3.743IleGly: 3.743 ± 0.428
1.628IleHis: 1.628 ± 0.379
1.356IleIle: 1.356 ± 0.287
1.194IleLys: 1.194 ± 0.276
1.899IleLeu: 1.899 ± 0.346
0.326IleMet: 0.326 ± 0.126
1.736IleAsn: 1.736 ± 0.308
2.93IlePro: 2.93 ± 0.318
1.248IleGln: 1.248 ± 0.228
2.658IleArg: 2.658 ± 0.394
1.899IleSer: 1.899 ± 0.411
3.472IleThr: 3.472 ± 0.426
3.092IleVal: 3.092 ± 0.359
0.814IleTrp: 0.814 ± 0.242
0.922IleTyr: 0.922 ± 0.233
0.0IleXaa: 0.0 ± 0.0
Lys
3.472LysAla: 3.472 ± 0.524
0.38LysCys: 0.38 ± 0.136
1.682LysAsp: 1.682 ± 0.305
1.139LysGlu: 1.139 ± 0.226
1.356LysPhe: 1.356 ± 0.239
2.333LysGly: 2.333 ± 0.439
1.031LysHis: 1.031 ± 0.238
0.76LysIle: 0.76 ± 0.196
1.411LysLys: 1.411 ± 0.343
2.387LysLeu: 2.387 ± 0.43
0.651LysMet: 0.651 ± 0.161
0.814LysAsn: 0.814 ± 0.199
2.604LysPro: 2.604 ± 0.402
1.519LysGln: 1.519 ± 0.248
2.658LysArg: 2.658 ± 0.435
2.387LysSer: 2.387 ± 0.299
2.17LysThr: 2.17 ± 0.33
2.496LysVal: 2.496 ± 0.307
0.922LysTrp: 0.922 ± 0.259
0.977LysTyr: 0.977 ± 0.259
0.0LysXaa: 0.0 ± 0.0
Leu
7.649LeuAla: 7.649 ± 0.712
0.868LeuCys: 0.868 ± 0.262
4.34LeuAsp: 4.34 ± 0.502
3.689LeuGlu: 3.689 ± 0.504
2.279LeuPhe: 2.279 ± 0.318
5.317LeuGly: 5.317 ± 0.562
1.139LeuHis: 1.139 ± 0.269
3.092LeuIle: 3.092 ± 0.433
2.17LeuLys: 2.17 ± 0.352
4.232LeuLeu: 4.232 ± 0.555
1.411LeuMet: 1.411 ± 0.26
2.387LeuAsn: 2.387 ± 0.355
5.371LeuPro: 5.371 ± 0.725
2.441LeuGln: 2.441 ± 0.398
5.045LeuArg: 5.045 ± 0.722
4.828LeuSer: 4.828 ± 0.558
5.642LeuThr: 5.642 ± 0.498
5.154LeuVal: 5.154 ± 0.457
0.814LeuTrp: 0.814 ± 0.188
2.224LeuTyr: 2.224 ± 0.391
0.0LeuXaa: 0.0 ± 0.0
Met
2.062MetAla: 2.062 ± 0.349
0.271MetCys: 0.271 ± 0.142
1.302MetAsp: 1.302 ± 0.283
0.868MetGlu: 0.868 ± 0.186
0.705MetPhe: 0.705 ± 0.209
1.573MetGly: 1.573 ± 0.279
0.109MetHis: 0.109 ± 0.082
0.868MetIle: 0.868 ± 0.219
0.922MetLys: 0.922 ± 0.243
1.682MetLeu: 1.682 ± 0.261
0.326MetMet: 0.326 ± 0.191
0.868MetAsn: 0.868 ± 0.229
1.194MetPro: 1.194 ± 0.207
0.651MetGln: 0.651 ± 0.173
1.845MetArg: 1.845 ± 0.371
2.875MetSer: 2.875 ± 0.396
2.55MetThr: 2.55 ± 0.37
1.302MetVal: 1.302 ± 0.279
0.38MetTrp: 0.38 ± 0.148
0.434MetTyr: 0.434 ± 0.141
0.0MetXaa: 0.0 ± 0.0
Asn
3.147AsnAla: 3.147 ± 0.396
0.217AsnCys: 0.217 ± 0.108
1.628AsnAsp: 1.628 ± 0.261
1.736AsnGlu: 1.736 ± 0.353
0.814AsnPhe: 0.814 ± 0.213
4.286AsnGly: 4.286 ± 0.552
0.977AsnHis: 0.977 ± 0.22
1.519AsnIle: 1.519 ± 0.455
1.085AsnLys: 1.085 ± 0.264
2.279AsnLeu: 2.279 ± 0.4
0.597AsnMet: 0.597 ± 0.16
1.845AsnAsn: 1.845 ± 0.354
2.55AsnPro: 2.55 ± 0.332
0.868AsnGln: 0.868 ± 0.32
1.845AsnArg: 1.845 ± 0.349
1.628AsnSer: 1.628 ± 0.308
2.062AsnThr: 2.062 ± 0.331
2.062AsnVal: 2.062 ± 0.345
0.814AsnTrp: 0.814 ± 0.163
0.38AsnTyr: 0.38 ± 0.118
0.0AsnXaa: 0.0 ± 0.0
Pro
5.045ProAla: 5.045 ± 0.547
0.76ProCys: 0.76 ± 0.208
4.286ProAsp: 4.286 ± 0.528
4.069ProGlu: 4.069 ± 0.427
1.519ProPhe: 1.519 ± 0.292
6.185ProGly: 6.185 ± 0.743
1.682ProHis: 1.682 ± 0.311
2.387ProIle: 2.387 ± 0.326
1.953ProLys: 1.953 ± 0.359
4.503ProLeu: 4.503 ± 0.497
1.302ProMet: 1.302 ± 0.298
2.062ProAsn: 2.062 ± 0.333
4.069ProPro: 4.069 ± 0.55
1.79ProGln: 1.79 ± 0.33
3.798ProArg: 3.798 ± 0.543
3.635ProSer: 3.635 ± 0.482
3.635ProThr: 3.635 ± 0.504
4.72ProVal: 4.72 ± 0.549
1.031ProTrp: 1.031 ± 0.25
1.682ProTyr: 1.682 ± 0.266
0.0ProXaa: 0.0 ± 0.0
Gln
4.611GlnAla: 4.611 ± 0.634
0.434GlnCys: 0.434 ± 0.188
1.411GlnAsp: 1.411 ± 0.248
1.628GlnGlu: 1.628 ± 0.333
1.139GlnPhe: 1.139 ± 0.221
2.713GlnGly: 2.713 ± 0.475
0.814GlnHis: 0.814 ± 0.251
1.953GlnIle: 1.953 ± 0.346
1.356GlnLys: 1.356 ± 0.263
2.713GlnLeu: 2.713 ± 0.46
0.814GlnMet: 0.814 ± 0.23
0.868GlnAsn: 0.868 ± 0.263
2.713GlnPro: 2.713 ± 0.4
1.139GlnGln: 1.139 ± 0.306
2.441GlnArg: 2.441 ± 0.324
2.007GlnSer: 2.007 ± 0.341
1.411GlnThr: 1.411 ± 0.243
2.17GlnVal: 2.17 ± 0.251
0.76GlnTrp: 0.76 ± 0.212
0.651GlnTyr: 0.651 ± 0.247
0.0GlnXaa: 0.0 ± 0.0
Arg
6.944ArgAla: 6.944 ± 0.665
1.573ArgCys: 1.573 ± 0.385
4.394ArgAsp: 4.394 ± 0.565
5.1ArgGlu: 5.1 ± 0.744
1.953ArgPhe: 1.953 ± 0.374
4.828ArgGly: 4.828 ± 0.474
1.356ArgHis: 1.356 ± 0.334
4.069ArgIle: 4.069 ± 0.644
2.441ArgLys: 2.441 ± 0.369
4.449ArgLeu: 4.449 ± 0.687
2.767ArgMet: 2.767 ± 0.432
2.387ArgAsn: 2.387 ± 0.43
4.015ArgPro: 4.015 ± 0.445
2.116ArgGln: 2.116 ± 0.425
6.076ArgArg: 6.076 ± 0.931
4.069ArgSer: 4.069 ± 0.419
3.96ArgThr: 3.96 ± 0.426
5.479ArgVal: 5.479 ± 0.544
1.899ArgTrp: 1.899 ± 0.359
2.116ArgTyr: 2.116 ± 0.266
0.0ArgXaa: 0.0 ± 0.0
Ser
5.534SerAla: 5.534 ± 0.955
0.597SerCys: 0.597 ± 0.194
4.394SerAsp: 4.394 ± 0.447
3.092SerGlu: 3.092 ± 0.476
2.062SerPhe: 2.062 ± 0.309
6.998SerGly: 6.998 ± 1.003
1.085SerHis: 1.085 ± 0.237
3.201SerIle: 3.201 ± 0.517
2.279SerLys: 2.279 ± 0.422
4.123SerLeu: 4.123 ± 0.45
1.194SerMet: 1.194 ± 0.243
2.333SerAsn: 2.333 ± 0.339
3.364SerPro: 3.364 ± 0.312
1.736SerGln: 1.736 ± 0.298
3.635SerArg: 3.635 ± 0.493
3.798SerSer: 3.798 ± 0.689
3.418SerThr: 3.418 ± 0.479
4.937SerVal: 4.937 ± 0.674
1.302SerTrp: 1.302 ± 0.248
1.628SerTyr: 1.628 ± 0.288
0.0SerXaa: 0.0 ± 0.0
Thr
7.595ThrAla: 7.595 ± 0.665
0.705ThrCys: 0.705 ± 0.237
4.449ThrAsp: 4.449 ± 0.677
3.526ThrGlu: 3.526 ± 0.346
1.628ThrPhe: 1.628 ± 0.272
5.805ThrGly: 5.805 ± 0.629
2.224ThrHis: 2.224 ± 0.326
3.472ThrIle: 3.472 ± 0.466
2.062ThrLys: 2.062 ± 0.399
3.852ThrLeu: 3.852 ± 0.439
1.248ThrMet: 1.248 ± 0.236
2.116ThrAsn: 2.116 ± 0.364
4.72ThrPro: 4.72 ± 0.556
2.387ThrGln: 2.387 ± 0.4
4.557ThrArg: 4.557 ± 0.518
4.123ThrSer: 4.123 ± 0.497
4.937ThrThr: 4.937 ± 0.615
6.076ThrVal: 6.076 ± 0.712
1.194ThrTrp: 1.194 ± 0.309
1.953ThrTyr: 1.953 ± 0.339
0.0ThrXaa: 0.0 ± 0.0
Val
7.704ValAla: 7.704 ± 0.738
1.194ValCys: 1.194 ± 0.256
5.425ValAsp: 5.425 ± 0.601
4.72ValGlu: 4.72 ± 0.571
2.333ValPhe: 2.333 ± 0.359
5.751ValGly: 5.751 ± 0.636
1.465ValHis: 1.465 ± 0.307
2.55ValIle: 2.55 ± 0.369
2.333ValLys: 2.333 ± 0.355
5.751ValLeu: 5.751 ± 0.59
1.139ValMet: 1.139 ± 0.198
2.062ValAsn: 2.062 ± 0.318
3.364ValPro: 3.364 ± 0.377
2.658ValGln: 2.658 ± 0.314
4.557ValArg: 4.557 ± 0.595
5.1ValSer: 5.1 ± 0.612
5.045ValThr: 5.045 ± 0.462
7.107ValVal: 7.107 ± 0.765
1.845ValTrp: 1.845 ± 0.358
1.519ValTyr: 1.519 ± 0.284
0.0ValXaa: 0.0 ± 0.0
Trp
1.953TrpAla: 1.953 ± 0.326
0.271TrpCys: 0.271 ± 0.115
1.356TrpAsp: 1.356 ± 0.278
1.085TrpGlu: 1.085 ± 0.298
0.543TrpPhe: 0.543 ± 0.153
1.031TrpGly: 1.031 ± 0.234
0.651TrpHis: 0.651 ± 0.205
1.031TrpIle: 1.031 ± 0.222
0.814TrpLys: 0.814 ± 0.16
1.953TrpLeu: 1.953 ± 0.333
1.031TrpMet: 1.031 ± 0.278
0.651TrpAsn: 0.651 ± 0.219
1.139TrpPro: 1.139 ± 0.289
1.031TrpGln: 1.031 ± 0.289
2.441TrpArg: 2.441 ± 0.389
1.356TrpSer: 1.356 ± 0.309
1.682TrpThr: 1.682 ± 0.318
1.519TrpVal: 1.519 ± 0.509
0.922TrpTrp: 0.922 ± 0.19
0.597TrpTyr: 0.597 ± 0.192
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.55TyrAla: 2.55 ± 0.424
0.543TyrCys: 0.543 ± 0.203
1.845TyrAsp: 1.845 ± 0.396
1.573TyrGlu: 1.573 ± 0.324
0.651TyrPhe: 0.651 ± 0.199
1.953TyrGly: 1.953 ± 0.408
0.271TyrHis: 0.271 ± 0.097
1.248TyrIle: 1.248 ± 0.216
0.705TyrLys: 0.705 ± 0.203
1.899TyrLeu: 1.899 ± 0.364
0.163TyrMet: 0.163 ± 0.093
0.705TyrAsn: 0.705 ± 0.185
1.411TyrPro: 1.411 ± 0.272
0.814TyrGln: 0.814 ± 0.203
2.062TyrArg: 2.062 ± 0.379
0.868TyrSer: 0.868 ± 0.18
2.224TyrThr: 2.224 ± 0.396
2.496TyrVal: 2.496 ± 0.342
0.76TyrTrp: 0.76 ± 0.214
0.543TyrTyr: 0.543 ± 0.161
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 109 proteins (18434 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski