candidate division TM7 genomosp. GTL1

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Saccharibacteria

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 670 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5KSH5|A5KSH5_9BACT Uncharacterized protein OS=candidate division TM7 genomosp. GTL1 OX=443342 GN=TM7_0245 PE=4 SV=1
MM1 pKa = 7.6AAMDD5 pKa = 3.84EE6 pKa = 4.46FANVLLPISIDD17 pKa = 3.54AQNGLAGASAAPTLPITTATANDD40 pKa = 4.28LLIGMVGVEE49 pKa = 4.29SDD51 pKa = 3.51SADD54 pKa = 3.36TYY56 pKa = 11.42TEE58 pKa = 4.05DD59 pKa = 5.35TIHH62 pKa = 6.04QWTSLTRR69 pKa = 11.84IGTTGGASNTNVTVNGVYY87 pKa = 10.08RR88 pKa = 11.84AVGSTGTYY96 pKa = 9.31TYY98 pKa = 11.14APTLGVSATWTEE110 pKa = 3.48FLIAYY115 pKa = 7.6KK116 pKa = 10.73ASS118 pKa = 3.29

Molecular weight: 12.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5KTI7|A5KTI7_9BACT Glyceraldehyde-3-phosphate dehydrogenase type I OS=candidate division TM7 genomosp. GTL1 OX=443342 GN=TM7_0629 PE=3 SV=1
MM1 pKa = 7.54TSACTNSILLYY12 pKa = 9.76CSNRR16 pKa = 11.84FGQRR20 pKa = 11.84RR21 pKa = 11.84IMANFEE27 pKa = 4.14LEE29 pKa = 4.35ANGHH33 pKa = 5.9HH34 pKa = 5.77VTTFKK39 pKa = 11.0AINTNQFWSGVRR51 pKa = 11.84KK52 pKa = 7.95NTPPGAKK59 pKa = 9.27NVQIFHH65 pKa = 6.9GNTLVAQGLSLRR77 pKa = 11.84VSEE80 pKa = 4.19RR81 pKa = 11.84QFRR84 pKa = 11.84EE85 pKa = 3.73ILVSS89 pKa = 3.48

Molecular weight: 10.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

670

0

670

178123

44

1093

265.9

29.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.657 ± 0.091

0.711 ± 0.025

5.423 ± 0.069

5.969 ± 0.091

4.17 ± 0.058

7.212 ± 0.104

2.067 ± 0.052

6.631 ± 0.07

5.848 ± 0.093

9.598 ± 0.116

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.166 ± 0.038

4.018 ± 0.064

4.272 ± 0.06

3.731 ± 0.058

5.474 ± 0.076

6.256 ± 0.082

6.246 ± 0.112

7.055 ± 0.08

1.233 ± 0.042

3.239 ± 0.059
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski