Blautia sp. CAG:257

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Blautia; environmental samples

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2516 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5X1N3|R5X1N3_9FIRM Uncharacterized protein OS=Blautia sp. CAG:257 OX=1262756 GN=BN568_02470 PE=3 SV=1
MM1 pKa = 7.34KK2 pKa = 10.24FIYY5 pKa = 9.69PAVFRR10 pKa = 11.84KK11 pKa = 8.36TEE13 pKa = 3.55NGTYY17 pKa = 10.15HH18 pKa = 7.05GFFPDD23 pKa = 5.26LEE25 pKa = 4.28CCYY28 pKa = 11.18ADD30 pKa = 5.79GDD32 pKa = 4.31TLDD35 pKa = 5.22DD36 pKa = 5.78AIDD39 pKa = 3.92NANEE43 pKa = 3.62AAYY46 pKa = 10.58NWISLEE52 pKa = 4.9LSEE55 pKa = 5.92DD56 pKa = 3.7DD57 pKa = 4.63CNLPSVSDD65 pKa = 3.87KK66 pKa = 11.4EE67 pKa = 4.82DD68 pKa = 3.36MEE70 pKa = 4.34LQEE73 pKa = 4.6GDD75 pKa = 3.02IVRR78 pKa = 11.84NISVNIRR85 pKa = 11.84FYY87 pKa = 11.03EE88 pKa = 4.51GWDD91 pKa = 3.35EE92 pKa = 4.06

Molecular weight: 10.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5WW25|R5WW25_9FIRM Guanylate kinase OS=Blautia sp. CAG:257 OX=1262756 GN=gmk PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.69VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.68GRR39 pKa = 11.84KK40 pKa = 8.28QLSAA44 pKa = 3.9

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2516

0

2516

801810

29

2665

318.7

35.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.576 ± 0.048

1.584 ± 0.023

5.387 ± 0.036

7.937 ± 0.063

4.08 ± 0.039

7.408 ± 0.043

1.767 ± 0.022

7.063 ± 0.047

6.718 ± 0.041

9.031 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.207 ± 0.021

4.023 ± 0.03

3.422 ± 0.026

3.397 ± 0.03

4.557 ± 0.037

5.726 ± 0.036

5.341 ± 0.034

6.98 ± 0.034

0.887 ± 0.015

3.91 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski