Ordospora colligata OC4
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1810 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B2UIJ9|A0A0B2UIJ9_9MICR Uncharacterized protein OS=Ordospora colligata OC4 OX=1354746 GN=M896_100530 PE=4 SV=1
MM1 pKa = 7.61 SSRR4 pKa = 11.84 FEE6 pKa = 4.27 PEE8 pKa = 3.31 YY9 pKa = 10.28 EE10 pKa = 4.02 AYY12 pKa = 10.26 FKK14 pKa = 10.96 RR15 pKa = 11.84 DD16 pKa = 3.57 VVPTDD21 pKa = 3.54 YY22 pKa = 11.28 NDD24 pKa = 3.46 EE25 pKa = 4.29 VNDD28 pKa = 3.59 YY29 pKa = 10.34 PVYY32 pKa = 10.99 DD33 pKa = 5.03 EE34 pKa = 5.89 IDD36 pKa = 3.44 MKK38 pKa = 11.23 DD39 pKa = 3.59 FEE41 pKa = 4.68 YY42 pKa = 11.18 SSANRR47 pKa = 11.84 TFYY50 pKa = 10.89 YY51 pKa = 9.86 PCPCGDD57 pKa = 3.45 RR58 pKa = 11.84 FEE60 pKa = 5.74 ISLDD64 pKa = 3.43 DD65 pKa = 4.06 LRR67 pKa = 11.84 NGEE70 pKa = 4.31 IIARR74 pKa = 11.84 CPSCSLLIRR83 pKa = 11.84 IVYY86 pKa = 10.12 EE87 pKa = 4.06 SDD89 pKa = 3.69 DD90 pKa = 3.81 LQAYY94 pKa = 7.26 EE95 pKa = 4.11
Molecular weight: 11.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.875
IPC2_protein 3.999
IPC_protein 3.948
Toseland 3.745
ProMoST 4.062
Dawson 3.923
Bjellqvist 4.126
Wikipedia 3.846
Rodwell 3.77
Grimsley 3.656
Solomon 3.91
Lehninger 3.872
Nozaki 4.05
DTASelect 4.24
Thurlkill 3.795
EMBOSS 3.859
Sillero 4.062
Patrickios 0.401
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.985
Protein with the highest isoelectric point:
>tr|A0A0B2UHZ0|A0A0B2UHZ0_9MICR Putative ubiquitin-protein ligase OS=Ordospora colligata OC4 OX=1354746 GN=M896_121860 PE=3 SV=1
MM1 pKa = 7.3 GAVNYY6 pKa = 10.47 LMEE9 pKa = 4.01 IHH11 pKa = 6.86 RR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 10.01 QSDD17 pKa = 3.37 VMRR20 pKa = 11.84 YY21 pKa = 8.17 LLRR24 pKa = 11.84 IRR26 pKa = 11.84 VCEE29 pKa = 3.56 YY30 pKa = 9.94 RR31 pKa = 11.84 QRR33 pKa = 11.84 GEE35 pKa = 3.97 CFRR38 pKa = 11.84 VEE40 pKa = 4.38 KK41 pKa = 9.12 PTFLDD46 pKa = 3.45 RR47 pKa = 11.84 ARR49 pKa = 11.84 SLGYY53 pKa = 9.52 KK54 pKa = 9.99 AKK56 pKa = 10.09 QGYY59 pKa = 7.86 VLYY62 pKa = 10.26 VARR65 pKa = 11.84 VKK67 pKa = 10.67 KK68 pKa = 10.91 GNLKK72 pKa = 10.37 RR73 pKa = 11.84 NYY75 pKa = 9.97 HH76 pKa = 5.88 NGNTRR81 pKa = 11.84 GKK83 pKa = 9.82 CVNAGIAQIKK93 pKa = 9.08 PSQRR97 pKa = 11.84 KK98 pKa = 6.68 QVNAEE103 pKa = 3.73 MVAGKK108 pKa = 9.87 KK109 pKa = 10.04 CSNLGVLNSYY119 pKa = 9.43 WIGHH123 pKa = 6.23 DD124 pKa = 3.22 AAYY127 pKa = 9.73 KK128 pKa = 8.07 YY129 pKa = 11.06 YY130 pKa = 10.22 EE131 pKa = 4.25 VIMVDD136 pKa = 3.26 RR137 pKa = 11.84 HH138 pKa = 5.88 HH139 pKa = 6.0 MAIRR143 pKa = 11.84 NDD145 pKa = 3.64 PKK147 pKa = 10.78 INWICRR153 pKa = 11.84 PTMKK157 pKa = 10.16 HH158 pKa = 5.37 RR159 pKa = 11.84 EE160 pKa = 4.2 CRR162 pKa = 11.84 GLTSAAKK169 pKa = 10.06 KK170 pKa = 10.52 SRR172 pKa = 11.84 GLGKK176 pKa = 9.87 GVRR179 pKa = 11.84 YY180 pKa = 9.77 NKK182 pKa = 10.5 SKK184 pKa = 10.13 GGSMRR189 pKa = 11.84 ACWRR193 pKa = 11.84 RR194 pKa = 11.84 RR195 pKa = 11.84 NTLVLQKK202 pKa = 10.7 YY203 pKa = 8.28 RR204 pKa = 3.68
Molecular weight: 23.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.955
IPC_protein 10.643
Toseland 10.716
ProMoST 10.467
Dawson 10.833
Bjellqvist 10.54
Wikipedia 11.038
Rodwell 11.169
Grimsley 10.891
Solomon 10.906
Lehninger 10.877
Nozaki 10.716
DTASelect 10.526
Thurlkill 10.73
EMBOSS 11.111
Sillero 10.76
Patrickios 10.862
IPC_peptide 10.921
IPC2_peptide 9.589
IPC2.peptide.svr19 8.37
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1810
0
1810
644518
51
3459
356.1
40.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.821 ± 0.037
2.408 ± 0.029
5.775 ± 0.038
7.969 ± 0.076
4.666 ± 0.042
5.534 ± 0.042
1.986 ± 0.023
7.725 ± 0.049
7.852 ± 0.063
8.549 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.789 ± 0.033
5.063 ± 0.038
2.591 ± 0.033
2.673 ± 0.031
5.272 ± 0.042
7.395 ± 0.049
4.061 ± 0.044
7.289 ± 0.057
0.698 ± 0.014
3.884 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here