Candidatus Scalindua rubra

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Candidatus Brocadiae; Candidatus Brocadiales; Candidatus Brocadiaceae; Candidatus Scalindua

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5207 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3X300|A0A1E3X300_9BACT Uncharacterized protein (Fragment) OS=Candidatus Scalindua rubra OX=1872076 GN=SCARUB_04923 PE=4 SV=1
MM1 pKa = 7.52NNNNLKK7 pKa = 10.34SCLILSIGVLLISTCEE23 pKa = 3.98EE24 pKa = 3.71PTEE27 pKa = 4.38PDD29 pKa = 3.18TTPPEE34 pKa = 4.17VTILDD39 pKa = 3.87PAEE42 pKa = 4.25GEE44 pKa = 4.33EE45 pKa = 4.04ISDD48 pKa = 3.78VFTVKK53 pKa = 9.48IDD55 pKa = 3.46ATDD58 pKa = 3.63DD59 pKa = 3.67NEE61 pKa = 4.06IEE63 pKa = 4.43KK64 pKa = 10.77VVLYY68 pKa = 10.37IDD70 pKa = 4.67FVPLDD75 pKa = 3.64SSLSEE80 pKa = 3.63KK81 pKa = 10.57DD82 pKa = 2.92IYY84 pKa = 11.24SFGWDD89 pKa = 3.31TDD91 pKa = 3.15IGDD94 pKa = 3.76NGEE97 pKa = 4.19YY98 pKa = 10.13TLLAQAFDD106 pKa = 3.45KK107 pKa = 11.26SGNKK111 pKa = 9.68SSSNPVNVTVINHH124 pKa = 7.15RR125 pKa = 11.84ILKK128 pKa = 9.13FVNTTWDD135 pKa = 4.35DD136 pKa = 3.05IDD138 pKa = 4.49FEE140 pKa = 4.51IAGVSDD146 pKa = 3.73VLLSLDD152 pKa = 4.02SIEE155 pKa = 5.21VEE157 pKa = 3.82IPKK160 pKa = 10.8NNGITNFYY168 pKa = 9.24GYY170 pKa = 10.18SWTGCGISIAWDD182 pKa = 3.3FDD184 pKa = 3.81IEE186 pKa = 4.85VGDD189 pKa = 3.99EE190 pKa = 3.88DD191 pKa = 5.36LRR193 pKa = 11.84WTVWVNPGAFYY204 pKa = 11.19LFLSNQSSVDD214 pKa = 3.1INYY217 pKa = 9.54VVVNKK222 pKa = 10.5DD223 pKa = 3.34LTDD226 pKa = 4.02LEE228 pKa = 4.94SWCYY232 pKa = 9.86WDD234 pKa = 4.12VPNDD238 pKa = 3.94GLVHH242 pKa = 6.59KK243 pKa = 9.9MGYY246 pKa = 8.86YY247 pKa = 9.29WADD250 pKa = 2.92

Molecular weight: 28.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3XE36|A0A1E3XE36_9BACT Methionyl-tRNA formyltransferase OS=Candidatus Scalindua rubra OX=1872076 GN=SCARUB_00954 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.19IIAQRR7 pKa = 11.84SRR9 pKa = 11.84NQNSFNVKK17 pKa = 7.25TQSSKK22 pKa = 10.71EE23 pKa = 3.5IRR25 pKa = 11.84TGVNVRR31 pKa = 11.84ITIFAYY37 pKa = 7.29QTPVCVRR44 pKa = 11.84LRR46 pKa = 11.84TGRR49 pKa = 11.84HH50 pKa = 4.73GGEE53 pKa = 3.98NLKK56 pKa = 10.61VPLQFAIVVRR66 pKa = 11.84LEE68 pKa = 4.1GVNLFYY74 pKa = 11.08DD75 pKa = 3.82LGEE78 pKa = 4.12IKK80 pKa = 10.84GDD82 pKa = 3.32GRR84 pKa = 11.84SANGG88 pKa = 3.16

Molecular weight: 9.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5207

0

5207

1364589

21

1692

262.1

29.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.767 ± 0.032

1.274 ± 0.015

5.498 ± 0.025

7.301 ± 0.037

4.588 ± 0.027

6.341 ± 0.033

1.96 ± 0.014

8.739 ± 0.035

8.461 ± 0.04

9.334 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.317 ± 0.016

4.897 ± 0.026

3.582 ± 0.021

2.809 ± 0.018

4.893 ± 0.025

6.206 ± 0.027

5.019 ± 0.025

6.277 ± 0.027

1.033 ± 0.012

3.705 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski