Clostridium chromiireducens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4771 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V4I934|A0A1V4I934_9CLOT N-(5'-phosphoribosyl)anthranilate isomerase OS=Clostridium chromiireducens OX=225345 GN=trpF PE=3 SV=1
MM1 pKa = 7.77SDD3 pKa = 3.17YY4 pKa = 11.67TMDD7 pKa = 2.89ISGNIEE13 pKa = 3.72LSDD16 pKa = 3.54YY17 pKa = 11.84SNIFDD22 pKa = 4.24YY23 pKa = 11.56LNIIDD28 pKa = 4.78KK29 pKa = 10.77DD30 pKa = 4.13DD31 pKa = 3.84NFVIRR36 pKa = 11.84VDD38 pKa = 3.8RR39 pKa = 11.84NNKK42 pKa = 9.44NDD44 pKa = 3.21INLINTMLLDD54 pKa = 3.53NQFIISYY61 pKa = 7.11TQYY64 pKa = 11.6DD65 pKa = 3.91DD66 pKa = 3.99LGNYY70 pKa = 8.71YY71 pKa = 9.94ISANKK76 pKa = 9.97CSSQNNII83 pKa = 3.62

Molecular weight: 9.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V4IIY5|A0A1V4IIY5_9CLOT Putative MscS family protein YkuT OS=Clostridium chromiireducens OX=225345 GN=ykuT PE=3 SV=1
MM1 pKa = 7.61SRR3 pKa = 11.84EE4 pKa = 3.76EE5 pKa = 4.22GNNNRR10 pKa = 11.84RR11 pKa = 11.84PGGKK15 pKa = 8.05MRR17 pKa = 11.84RR18 pKa = 11.84SRR20 pKa = 11.84KK21 pKa = 9.11KK22 pKa = 8.92VCAFCVDD29 pKa = 3.07KK30 pKa = 11.69AEE32 pKa = 4.83FIDD35 pKa = 4.01YY36 pKa = 11.0KK37 pKa = 11.0DD38 pKa = 3.24INKK41 pKa = 9.06LRR43 pKa = 11.84KK44 pKa = 9.21YY45 pKa = 7.83VTEE48 pKa = 4.22RR49 pKa = 11.84GKK51 pKa = 10.15ILPRR55 pKa = 11.84RR56 pKa = 11.84ISGTCAKK63 pKa = 9.87HH64 pKa = 5.43QRR66 pKa = 11.84EE67 pKa = 4.29LTASIKK73 pKa = 9.88RR74 pKa = 11.84ARR76 pKa = 11.84NIALLPFTTEE86 pKa = 3.59

Molecular weight: 10.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4771

0

4771

1479602

29

3077

310.1

34.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.761 ± 0.042

1.165 ± 0.014

5.593 ± 0.03

7.452 ± 0.043

4.335 ± 0.029

6.424 ± 0.039

1.374 ± 0.014

9.908 ± 0.041

8.625 ± 0.036

8.973 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.614 ± 0.017

6.468 ± 0.036

2.763 ± 0.02

2.448 ± 0.018

3.303 ± 0.022

6.605 ± 0.03

5.001 ± 0.028

6.328 ± 0.03

0.774 ± 0.015

4.086 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski