Sulfolobus monocaudavirus SMV3
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N9P6R5|A0A0N9P6R5_9VIRU Uncharacterized protein OS=Sulfolobus monocaudavirus SMV3 OX=1732177 PE=4 SV=1
MM1 pKa = 7.5 EE2 pKa = 5.46 PSEE5 pKa = 3.99 VAGIIIEE12 pKa = 4.23 RR13 pKa = 11.84 VVDD16 pKa = 3.56 YY17 pKa = 7.82 CTNISGDD24 pKa = 3.63 TVLDD28 pKa = 3.56 CLEE31 pKa = 4.22 YY32 pKa = 10.63 HH33 pKa = 7.12 SPDD36 pKa = 3.44 QLDD39 pKa = 3.87 TFVDD43 pKa = 4.01 MYY45 pKa = 10.52 LTEE48 pKa = 4.6 EE49 pKa = 4.64 EE50 pKa = 4.66 DD51 pKa = 4.04 NEE53 pKa = 4.13 LQKK56 pKa = 11.15 EE57 pKa = 3.93 IDD59 pKa = 3.52 KK60 pKa = 11.26 SEE62 pKa = 4.12 KK63 pKa = 10.25 FWDD66 pKa = 4.02 EE67 pKa = 3.81 VKK69 pKa = 10.72 KK70 pKa = 10.62 EE71 pKa = 3.92 YY72 pKa = 10.5 EE73 pKa = 4.18 KK74 pKa = 11.0 LWEE77 pKa = 4.02 QEE79 pKa = 3.41 MDD81 pKa = 4.24 LEE83 pKa = 4.96 LDD85 pKa = 3.71 SYY87 pKa = 12.09 ADD89 pKa = 3.52 DD90 pKa = 3.81 TRR92 pKa = 11.84 DD93 pKa = 3.59 YY94 pKa = 11.45 LADD97 pKa = 3.89 TLSMLYY103 pKa = 10.68 EE104 pKa = 4.28 NIIQNDD110 pKa = 4.09 EE111 pKa = 4.3 LSDD114 pKa = 3.46 TCKK117 pKa = 10.5 ARR119 pKa = 11.84 RR120 pKa = 11.84 LKK122 pKa = 10.68 AYY124 pKa = 10.43 AEE126 pKa = 4.05 VLEE129 pKa = 4.39 EE130 pKa = 4.46 FGNAINRR137 pKa = 11.84 ALKK140 pKa = 10.63 DD141 pKa = 3.69 GEE143 pKa = 4.42 SLSDD147 pKa = 3.09 IDD149 pKa = 4.65 LDD151 pKa = 3.38 YY152 pKa = 10.52 WYY154 pKa = 10.89 RR155 pKa = 11.84 KK156 pKa = 9.74 IGDD159 pKa = 3.27 IAFGVKK165 pKa = 9.72 NYY167 pKa = 10.56 NYY169 pKa = 10.1 YY170 pKa = 10.93 DD171 pKa = 4.15 EE172 pKa = 5.25 IDD174 pKa = 3.84 PYY176 pKa = 10.75 IYY178 pKa = 10.86 YY179 pKa = 9.8 YY180 pKa = 11.49 DD181 pKa = 3.72 NMSKK185 pKa = 10.57 NKK187 pKa = 9.79 KK188 pKa = 10.38 LEE190 pKa = 4.05 VLSALLDD197 pKa = 4.36 EE198 pKa = 5.36 IGNVLYY204 pKa = 10.53 DD205 pKa = 3.91 IEE207 pKa = 5.23 HH208 pKa = 7.23 KK209 pKa = 10.92 LPEE212 pKa = 4.93 LEE214 pKa = 4.91 GLTNNNSS221 pKa = 3.09
Molecular weight: 25.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.893
IPC2_protein 3.999
IPC_protein 3.986
Toseland 3.795
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.859
Rodwell 3.821
Grimsley 3.694
Solomon 3.948
Lehninger 3.91
Nozaki 4.062
DTASelect 4.253
Thurlkill 3.821
EMBOSS 3.872
Sillero 4.101
Patrickios 2.486
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 3.993
Protein with the highest isoelectric point:
>tr|A0A0N9P4T9|A0A0N9P4T9_9VIRU Uncharacterized protein OS=Sulfolobus monocaudavirus SMV3 OX=1732177 PE=4 SV=1
MM1 pKa = 7.26 KK2 pKa = 9.35 RR3 pKa = 11.84 TYY5 pKa = 10.14 FLCKK9 pKa = 9.73 FLEE12 pKa = 4.21 EE13 pKa = 4.12 NKK15 pKa = 9.78 VFEE18 pKa = 4.75 GKK20 pKa = 9.86 FRR22 pKa = 11.84 ACRR25 pKa = 11.84 GYY27 pKa = 10.02 IDD29 pKa = 3.76 VGHH32 pKa = 5.98 NVKK35 pKa = 10.21 IGEE38 pKa = 4.17 EE39 pKa = 3.9 YY40 pKa = 10.57 AGYY43 pKa = 10.07 KK44 pKa = 9.23 EE45 pKa = 5.63 GYY47 pKa = 8.22 ICSEE51 pKa = 3.89 EE52 pKa = 4.32 IIWKK56 pKa = 9.97 KK57 pKa = 10.85 DD58 pKa = 3.0 GTLEE62 pKa = 3.9 LRR64 pKa = 11.84 RR65 pKa = 11.84 RR66 pKa = 11.84 KK67 pKa = 9.36 LRR69 pKa = 11.84 KK70 pKa = 8.15 RR71 pKa = 11.84 TLEE74 pKa = 3.55 IVRR77 pKa = 11.84 RR78 pKa = 11.84 DD79 pKa = 2.99 TDD81 pKa = 3.6 LYY83 pKa = 11.18 GEE85 pKa = 4.2 LRR87 pKa = 11.84 SRR89 pKa = 11.84 RR90 pKa = 11.84 HH91 pKa = 5.15 IPKK94 pKa = 9.93 GCRR97 pKa = 3.03
Molecular weight: 11.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.189
IPC2_protein 9.063
IPC_protein 9.063
Toseland 9.706
ProMoST 9.575
Dawson 9.955
Bjellqvist 9.692
Wikipedia 10.131
Rodwell 10.262
Grimsley 10.028
Solomon 9.999
Lehninger 9.97
Nozaki 9.838
DTASelect 9.648
Thurlkill 9.809
EMBOSS 10.131
Sillero 9.911
Patrickios 7.6
IPC_peptide 9.999
IPC2_peptide 8.521
IPC2.peptide.svr19 8.054
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
87
0
87
18332
42
1175
210.7
23.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.948 ± 0.276
0.556 ± 0.094
4.915 ± 0.332
7.195 ± 0.655
4.528 ± 0.224
4.92 ± 0.401
1.091 ± 0.114
8.166 ± 0.326
7.135 ± 0.534
9.923 ± 0.341
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.057 ± 0.139
5.924 ± 0.339
3.791 ± 0.267
4.309 ± 0.615
3.224 ± 0.255
7.206 ± 0.446
6.29 ± 0.363
6.895 ± 0.331
0.786 ± 0.118
5.122 ± 0.254
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here