Eimeria tenella RNA virus 1
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7DRF5|A0A0A7DRF5_9VIRU RNA-directed RNA polymerase OS=Eimeria tenella RNA virus 1 OX=1566868 GN=RDRP PE=3 SV=1
MM1 pKa = 7.74 AEE3 pKa = 4.14 ADD5 pKa = 3.7 RR6 pKa = 11.84 SGMVSGAQSILGLCRR21 pKa = 11.84 NDD23 pKa = 3.97 FLAPPDD29 pKa = 3.21 IGRR32 pKa = 11.84 CRR34 pKa = 11.84 VYY36 pKa = 10.05 MGSVLLTWRR45 pKa = 11.84 AYY47 pKa = 10.62 GKK49 pKa = 9.63 MKK51 pKa = 10.58 TNIHH55 pKa = 6.02 KK56 pKa = 10.25 IKK58 pKa = 11.02 YY59 pKa = 7.53 EE60 pKa = 3.7 VGYY63 pKa = 10.33 RR64 pKa = 11.84 WDD66 pKa = 3.75 GEE68 pKa = 3.9 AARR71 pKa = 11.84 PGKK74 pKa = 9.89 RR75 pKa = 11.84 DD76 pKa = 3.19 PALVRR81 pKa = 11.84 NSSEE85 pKa = 3.71 WATKK89 pKa = 10.61 GDD91 pKa = 3.45 ILPRR95 pKa = 11.84 VHH97 pKa = 7.02 AVTPADD103 pKa = 3.52 YY104 pKa = 10.97 LEE106 pKa = 4.58 RR107 pKa = 11.84 LIKK110 pKa = 10.39 VEE112 pKa = 4.23 PKK114 pKa = 8.97 HH115 pKa = 5.6 TSRR118 pKa = 11.84 PDD120 pKa = 2.75 WTHH123 pKa = 6.57 FDD125 pKa = 3.44 FTGPVAMLGAWLATLTWFPGVSLFDD150 pKa = 4.46 LYY152 pKa = 11.34 DD153 pKa = 3.68 PEE155 pKa = 5.64 KK156 pKa = 10.87 IKK158 pKa = 10.76 INHH161 pKa = 6.66 LNQLPDD167 pKa = 3.77 SSALSCNTVFLSQQLSGQLPSGCYY191 pKa = 7.76 WVLVSACGACGSSVATDD208 pKa = 3.48 RR209 pKa = 11.84 AILSGPEE216 pKa = 3.46 RR217 pKa = 11.84 ARR219 pKa = 11.84 LFGAQGPKK227 pKa = 9.42 LWKK230 pKa = 10.4 DD231 pKa = 2.89 ILDD234 pKa = 3.94 ALRR237 pKa = 11.84 LLGEE241 pKa = 4.38 LYY243 pKa = 10.78 DD244 pKa = 3.61 RR245 pKa = 11.84 AGAGEE250 pKa = 4.13 VFGYY254 pKa = 10.81 AVAKK258 pKa = 10.17 GLHH261 pKa = 5.0 TVNTVVSHH269 pKa = 6.26 SDD271 pKa = 2.94 EE272 pKa = 4.76 GGLFRR277 pKa = 11.84 DD278 pKa = 3.73 VLRR281 pKa = 11.84 EE282 pKa = 3.86 CRR284 pKa = 11.84 FALPFGGIPPDD295 pKa = 3.89 SPLSGSLPTVTISATYY311 pKa = 7.53 QTLVTIVDD319 pKa = 4.04 SLSLTTAAAVAQAGTVVTTDD339 pKa = 2.72 TGTTRR344 pKa = 11.84 PIVVYY349 pKa = 10.87 GRR351 pKa = 11.84 MTPLMARR358 pKa = 11.84 GVRR361 pKa = 11.84 PADD364 pKa = 3.38 PAIVGDD370 pKa = 3.8 ADD372 pKa = 3.97 RR373 pKa = 11.84 YY374 pKa = 11.11 ARR376 pKa = 11.84 DD377 pKa = 3.38 QEE379 pKa = 4.41 QYY381 pKa = 11.27 RR382 pKa = 11.84 LDD384 pKa = 3.83 CTEE387 pKa = 4.06 HH388 pKa = 6.46 VNLLRR393 pKa = 11.84 APLHH397 pKa = 5.3 GAYY400 pKa = 9.24 TEE402 pKa = 3.92 MAEE405 pKa = 4.32 YY406 pKa = 10.53 YY407 pKa = 10.59 LPALANIFRR416 pKa = 11.84 MSRR419 pKa = 11.84 AAEE422 pKa = 4.09 EE423 pKa = 3.93 TRR425 pKa = 11.84 PAHH428 pKa = 5.46 TWLSQAAPSVLEE440 pKa = 4.42 NYY442 pKa = 10.06 DD443 pKa = 3.4 NRR445 pKa = 11.84 HH446 pKa = 5.46 LLKK449 pKa = 10.26 NDD451 pKa = 2.89 AVAPFFWIEE460 pKa = 3.78 PTTILPRR467 pKa = 11.84 KK468 pKa = 7.64 WCLGPAEE475 pKa = 4.21 TGGWGSLGGPGEE487 pKa = 4.46 AYY489 pKa = 9.8 SEE491 pKa = 4.06 RR492 pKa = 11.84 MFPNLCEE499 pKa = 3.87 VDD501 pKa = 3.56 IPTATARR508 pKa = 11.84 SYY510 pKa = 11.14 EE511 pKa = 3.8 IDD513 pKa = 3.42 FEE515 pKa = 4.5 GARR518 pKa = 11.84 KK519 pKa = 9.07 CAWLICLLGHH529 pKa = 6.75 KK530 pKa = 9.88 EE531 pKa = 3.99 DD532 pKa = 5.38 GLAQMHH538 pKa = 6.72 FKK540 pKa = 10.85 RR541 pKa = 11.84 LDD543 pKa = 3.51 PEE545 pKa = 4.3 ALCLLGGRR553 pKa = 11.84 AARR556 pKa = 11.84 DD557 pKa = 3.86 GPGRR561 pKa = 11.84 AHH563 pKa = 6.66 SARR566 pKa = 11.84 YY567 pKa = 8.13 PVSDD571 pKa = 3.93 YY572 pKa = 11.34 LWVRR576 pKa = 11.84 GQSKK580 pKa = 10.21 LPHH583 pKa = 6.47 PAEE586 pKa = 4.45 CLNMFDD592 pKa = 4.01 RR593 pKa = 11.84 VVMVINHH600 pKa = 5.9 YY601 pKa = 10.05 KK602 pKa = 8.8 VQEE605 pKa = 4.14 DD606 pKa = 3.32 RR607 pKa = 11.84 WLATVPSHH615 pKa = 6.88 NEE617 pKa = 3.33 IAGATYY623 pKa = 9.58 TVDD626 pKa = 3.06 VSAPDD631 pKa = 3.14 SCGFAEE637 pKa = 4.48 RR638 pKa = 11.84 CAEE641 pKa = 3.88 TRR643 pKa = 11.84 RR644 pKa = 11.84 EE645 pKa = 3.83 HH646 pKa = 5.61 MARR649 pKa = 11.84 TRR651 pKa = 11.84 GADD654 pKa = 3.07 ALMAMASLLNADD666 pKa = 5.09 DD667 pKa = 4.89 PNSLHH672 pKa = 6.55 EE673 pKa = 4.62 GGYY676 pKa = 8.01 LTVGIRR682 pKa = 11.84 RR683 pKa = 11.84 RR684 pKa = 11.84 QGAATPAPPLRR695 pKa = 11.84 SQAGGPQSTQVEE707 pKa = 4.74 APTQSAGHH715 pKa = 5.29 TAEE718 pKa = 4.43 AAAPQPSRR726 pKa = 11.84 VLTAASAPMSPVLAAGTHH744 pKa = 6.09 AGPRR748 pKa = 11.84 VPQTGGGQPGPEE760 pKa = 3.93 SVQVAPASNNPDD772 pKa = 3.12 GQTEE776 pKa = 4.42 GGGGAQGTAPTQQ788 pKa = 3.22
Molecular weight: 84.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.029
IPC2_protein 6.008
IPC_protein 6.084
Toseland 6.402
ProMoST 6.465
Dawson 6.415
Bjellqvist 6.415
Wikipedia 6.44
Rodwell 6.415
Grimsley 6.532
Solomon 6.415
Lehninger 6.415
Nozaki 6.707
DTASelect 6.883
Thurlkill 6.898
EMBOSS 6.868
Sillero 6.81
Patrickios 4.088
IPC_peptide 6.44
IPC2_peptide 6.766
IPC2.peptide.svr19 6.701
Protein with the highest isoelectric point:
>tr|A0A0A7DRF5|A0A0A7DRF5_9VIRU RNA-directed RNA polymerase OS=Eimeria tenella RNA virus 1 OX=1566868 GN=RDRP PE=3 SV=1
MM1 pKa = 7.26 MVPKK5 pKa = 9.89 EE6 pKa = 4.19 ATASHH11 pKa = 7.15 PPGALWGAVSKK22 pKa = 11.13 SGVLSVLGRR31 pKa = 11.84 SFHH34 pKa = 6.83 AAGNMASKK42 pKa = 10.54 FDD44 pKa = 3.96 ADD46 pKa = 4.02 VIRR49 pKa = 11.84 SEE51 pKa = 4.67 LVSAAQGQPSEE62 pKa = 4.25 DD63 pKa = 2.91 SGYY66 pKa = 9.5 ATLVAYY72 pKa = 10.37 NLAYY76 pKa = 10.45 NPYY79 pKa = 8.91 PIHH82 pKa = 7.06 LPLPLSWRR90 pKa = 11.84 SEE92 pKa = 4.16 VIRR95 pKa = 11.84 WLTYY99 pKa = 9.68 YY100 pKa = 11.08 SLITMSSWWVATDD113 pKa = 3.98 VVGFWSSGGFCEE125 pKa = 4.73 IAPADD130 pKa = 3.91 FVEE133 pKa = 4.89 RR134 pKa = 11.84 AAPRR138 pKa = 11.84 VNPLEE143 pKa = 4.15 AGVRR147 pKa = 11.84 LEE149 pKa = 4.43 SLVKK153 pKa = 10.29 AYY155 pKa = 10.33 RR156 pKa = 11.84 DD157 pKa = 3.44 RR158 pKa = 11.84 ASYY161 pKa = 10.58 ASRR164 pKa = 11.84 VGPEE168 pKa = 3.12 LHH170 pKa = 6.54 RR171 pKa = 11.84 RR172 pKa = 11.84 YY173 pKa = 9.47 IAPDD177 pKa = 3.48 DD178 pKa = 4.13 GGNPFRR184 pKa = 11.84 ALQAGSPSRR193 pKa = 11.84 ARR195 pKa = 11.84 LQSPRR200 pKa = 11.84 VRR202 pKa = 11.84 PARR205 pKa = 11.84 RR206 pKa = 11.84 KK207 pKa = 8.55 RR208 pKa = 11.84 AQWRR212 pKa = 11.84 CTAFADD218 pKa = 4.49 VIAEE222 pKa = 4.0 ITGLVCGQLKK232 pKa = 8.46 TLYY235 pKa = 10.55 SGVRR239 pKa = 11.84 EE240 pKa = 4.07 YY241 pKa = 11.41 ARR243 pKa = 11.84 SEE245 pKa = 4.03 VYY247 pKa = 9.9 EE248 pKa = 3.87 AVMRR252 pKa = 11.84 CVNRR256 pKa = 11.84 ALCHH260 pKa = 5.39 AHH262 pKa = 6.92 LSGGEE267 pKa = 4.16 GSAGGAGPGAMRR279 pKa = 11.84 HH280 pKa = 4.65 QSEE283 pKa = 4.6 SGRR286 pKa = 11.84 SVPWRR291 pKa = 11.84 PQAHH295 pKa = 6.28 EE296 pKa = 4.86 AGSSAPWTHH305 pKa = 6.76 RR306 pKa = 11.84 VPEE309 pKa = 4.36 SYY311 pKa = 10.36 LAEE314 pKa = 3.98 VAEE317 pKa = 5.0 RR318 pKa = 11.84 ISPRR322 pKa = 11.84 DD323 pKa = 3.49 VLSEE327 pKa = 4.16 LIARR331 pKa = 11.84 DD332 pKa = 3.19 SSIALRR338 pKa = 11.84 QEE340 pKa = 4.0 LGRR343 pKa = 11.84 LFPRR347 pKa = 11.84 KK348 pKa = 9.21 RR349 pKa = 11.84 DD350 pKa = 3.61 PQSGRR355 pKa = 11.84 KK356 pKa = 9.06 ANIYY360 pKa = 10.14 LDD362 pKa = 4.21 CLLADD367 pKa = 4.62 IRR369 pKa = 11.84 DD370 pKa = 3.75 IDD372 pKa = 4.19 PASHH376 pKa = 6.55 RR377 pKa = 11.84 QLCMMLSAAPPGAIHH392 pKa = 7.33 EE393 pKa = 4.46 DD394 pKa = 3.75 QVCNALIYY402 pKa = 10.26 AYY404 pKa = 9.61 SLWRR408 pKa = 11.84 GRR410 pKa = 11.84 EE411 pKa = 3.79 ALLGAFCILVNLAEE425 pKa = 4.52 GGEE428 pKa = 4.22 RR429 pKa = 11.84 LKK431 pKa = 11.06 HH432 pKa = 6.22 LSDD435 pKa = 3.2 ILKK438 pKa = 10.48 ASGSNTVFEE447 pKa = 4.33 WAQLCEE453 pKa = 4.36 LNCLLGRR460 pKa = 11.84 GVGDD464 pKa = 3.33 TDD466 pKa = 3.29 LWSDD470 pKa = 3.44 AEE472 pKa = 4.11 RR473 pKa = 11.84 RR474 pKa = 11.84 TRR476 pKa = 11.84 PEE478 pKa = 3.25 KK479 pKa = 11.05 DD480 pKa = 3.02 MEE482 pKa = 4.32 VEE484 pKa = 3.73 LDD486 pKa = 3.24 RR487 pKa = 11.84 RR488 pKa = 11.84 RR489 pKa = 11.84 LSDD492 pKa = 4.66 AINAVLAEE500 pKa = 4.12 EE501 pKa = 5.25 LGDD504 pKa = 4.06 EE505 pKa = 4.71 PLHH508 pKa = 6.84 ADD510 pKa = 5.09 PIQDD514 pKa = 2.97 YY515 pKa = 9.51 WDD517 pKa = 4.73 RR518 pKa = 11.84 RR519 pKa = 11.84 FEE521 pKa = 3.9 WCVAGSHH528 pKa = 5.59 NHH530 pKa = 4.27 TTNAEE535 pKa = 3.89 GAFADD540 pKa = 4.63 VPATGPGGVRR550 pKa = 11.84 MTRR553 pKa = 11.84 RR554 pKa = 11.84 MAMEE558 pKa = 4.41 HH559 pKa = 5.76 VDD561 pKa = 3.77 EE562 pKa = 4.75 NALVTWDD569 pKa = 3.34 GHH571 pKa = 4.56 VQVSVVPKK579 pKa = 10.47 LEE581 pKa = 3.93 QGKK584 pKa = 7.48 TRR586 pKa = 11.84 AIYY589 pKa = 10.02 SCNTVSYY596 pKa = 10.33 AAFGRR601 pKa = 11.84 ILRR604 pKa = 11.84 PAEE607 pKa = 3.77 NRR609 pKa = 11.84 WAGRR613 pKa = 11.84 RR614 pKa = 11.84 VILDD618 pKa = 3.53 PGAGGNYY625 pKa = 10.08 GMFRR629 pKa = 11.84 RR630 pKa = 11.84 IRR632 pKa = 11.84 KK633 pKa = 7.83 AWPRR637 pKa = 11.84 ALGTALMLDD646 pKa = 3.64 YY647 pKa = 11.43 SDD649 pKa = 5.95 FNSQHH654 pKa = 5.34 TLAAQEE660 pKa = 4.35 LVVKK664 pKa = 8.45 LLCDD668 pKa = 3.16 RR669 pKa = 11.84 FTNLEE674 pKa = 3.93 PDD676 pKa = 3.46 YY677 pKa = 11.59 SEE679 pKa = 5.62 LLVSSLHH686 pKa = 7.02 KK687 pKa = 9.9 MDD689 pKa = 4.14 IYY691 pKa = 11.55 VHH693 pKa = 6.08 GKK695 pKa = 9.12 LVGRR699 pKa = 11.84 ARR701 pKa = 11.84 RR702 pKa = 11.84 SLMSDD707 pKa = 2.6 HH708 pKa = 7.2 RR709 pKa = 11.84 GTSFINSVLNAAYY722 pKa = 9.2 IRR724 pKa = 11.84 YY725 pKa = 9.07 VLGEE729 pKa = 3.53 EE730 pKa = 5.26 RR731 pKa = 11.84 YY732 pKa = 10.11 RR733 pKa = 11.84 KK734 pKa = 8.17 MQSFHH739 pKa = 8.01 VGDD742 pKa = 4.44 DD743 pKa = 3.19 VLMFCKK749 pKa = 10.48 DD750 pKa = 3.15 SVEE753 pKa = 4.19 AYY755 pKa = 10.05 GVIGAMEE762 pKa = 4.38 DD763 pKa = 3.43 AGFHH767 pKa = 5.43 LQRR770 pKa = 11.84 SKK772 pKa = 11.3 QSVGSKK778 pKa = 10.08 GFEE781 pKa = 4.21 FLRR784 pKa = 11.84 MAGTRR789 pKa = 11.84 SLAHH793 pKa = 6.5 GYY795 pKa = 7.47 VARR798 pKa = 11.84 SIASLVSGNWTTDD811 pKa = 2.46 WTYY814 pKa = 11.85 DD815 pKa = 3.44 PVATMHH821 pKa = 6.86 SFVQQARR828 pKa = 11.84 SLMNRR833 pKa = 11.84 SGNVSAYY840 pKa = 10.38 LLLGRR845 pKa = 11.84 SMQVYY850 pKa = 8.51 TSLPLGVIHH859 pKa = 6.57 EE860 pKa = 4.45 VLSGRR865 pKa = 11.84 VALGAGPVYY874 pKa = 10.41 RR875 pKa = 11.84 ADD877 pKa = 3.09 GRR879 pKa = 11.84 YY880 pKa = 5.99 EE881 pKa = 3.65 TRR883 pKa = 11.84 AILEE887 pKa = 4.05 VGDD890 pKa = 3.72 YY891 pKa = 10.53 NVRR894 pKa = 11.84 EE895 pKa = 4.52 DD896 pKa = 3.61 LPTWRR901 pKa = 11.84 RR902 pKa = 11.84 LPRR905 pKa = 11.84 AATADD910 pKa = 3.65 YY911 pKa = 7.47 FTRR914 pKa = 11.84 GCHH917 pKa = 4.84 AVEE920 pKa = 3.98 RR921 pKa = 11.84 LAMEE925 pKa = 4.52 LVGFQPWAAALRR937 pKa = 11.84 ASYY940 pKa = 10.44 GALAVRR946 pKa = 11.84 RR947 pKa = 11.84 LQEE950 pKa = 3.94 GLACRR955 pKa = 11.84 DD956 pKa = 3.51 PAADD960 pKa = 3.35 IPTSVTLGCKK970 pKa = 8.62 QLYY973 pKa = 9.26 CKK975 pKa = 10.55 AGAVDD980 pKa = 5.59 LDD982 pKa = 4.24 SEE984 pKa = 4.73 LDD986 pKa = 3.35 RR987 pKa = 11.84 PTRR990 pKa = 11.84 RR991 pKa = 11.84 GALVQYY997 pKa = 9.11 PVIALLQNTLTDD1009 pKa = 3.93 GQIGEE1014 pKa = 4.46 LLRR1017 pKa = 11.84 YY1018 pKa = 10.05 LGVPFQAGQEE1028 pKa = 4.13 RR1029 pKa = 11.84 ITAFGNPSVGVVIQGDD1045 pKa = 4.25 LPYY1048 pKa = 10.58 SDD1050 pKa = 5.94 ASALCSKK1057 pKa = 10.91 GILQTVRR1064 pKa = 11.84 VIYY1067 pKa = 8.76 PLRR1070 pKa = 11.84 MM1071 pKa = 3.63
Molecular weight: 118.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.424
IPC2_protein 7.732
IPC_protein 7.527
Toseland 7.176
ProMoST 8.112
Dawson 8.214
Bjellqvist 8.653
Wikipedia 8.141
Rodwell 8.229
Grimsley 7.249
Solomon 8.244
Lehninger 8.258
Nozaki 8.77
DTASelect 8.346
Thurlkill 8.375
EMBOSS 8.419
Sillero 8.712
Patrickios 4.304
IPC_peptide 8.244
IPC2_peptide 7.863
IPC2.peptide.svr19 7.829
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1859
788
1071
929.5
101.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.565 ± 0.29
1.937 ± 0.055
5.272 ± 0.034
5.218 ± 0.159
2.474 ± 0.037
8.714 ± 0.324
2.582 ± 0.049
3.335 ± 0.171
2.367 ± 0.101
9.736 ± 0.279
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.259 ± 0.06
2.582 ± 0.026
5.971 ± 0.895
3.228 ± 0.042
8.338 ± 0.802
6.993 ± 0.532
4.949 ± 0.974
7.047 ± 0.414
1.937 ± 0.019
3.497 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here