Tortoise microvirus 58
Average proteome isoelectric point is 5.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W6S8|A0A4P8W6S8_9VIRU Major capsid protein OS=Tortoise microvirus 58 OX=2583163 PE=3 SV=1
MM1 pKa = 7.72 DD2 pKa = 3.39 VAYY5 pKa = 10.15 EE6 pKa = 4.4 RR7 pKa = 11.84 IHH9 pKa = 7.07 GLTCAPNVALAIRR22 pKa = 11.84 DD23 pKa = 3.41 ISPTLKK29 pKa = 10.08 RR30 pKa = 11.84 VAPHH34 pKa = 6.51 FLDD37 pKa = 4.85 DD38 pKa = 3.66 MEE40 pKa = 4.91 LRR42 pKa = 11.84 EE43 pKa = 4.24 IGYY46 pKa = 9.96 FDD48 pKa = 4.71 SDD50 pKa = 4.7 LEE52 pKa = 4.11 FHH54 pKa = 6.66 PTPAVVHH61 pKa = 6.09 SWDD64 pKa = 3.66 EE65 pKa = 4.23 YY66 pKa = 11.26 KK67 pKa = 11.07 DD68 pKa = 3.51 PEE70 pKa = 4.57 QPVTPLSDD78 pKa = 3.42 GQLKK82 pKa = 10.6 DD83 pKa = 3.65 FQRR86 pKa = 5.05
Molecular weight: 9.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.62
IPC2_protein 4.647
IPC_protein 4.546
Toseland 4.406
ProMoST 4.698
Dawson 4.533
Bjellqvist 4.685
Wikipedia 4.444
Rodwell 4.406
Grimsley 4.317
Solomon 4.533
Lehninger 4.482
Nozaki 4.647
DTASelect 4.863
Thurlkill 4.431
EMBOSS 4.469
Sillero 4.685
Patrickios 3.821
IPC_peptide 4.533
IPC2_peptide 4.673
IPC2.peptide.svr19 4.625
Protein with the highest isoelectric point:
>tr|A0A4P8W9U0|A0A4P8W9U0_9VIRU Uncharacterized protein OS=Tortoise microvirus 58 OX=2583163 PE=4 SV=1
MM1 pKa = 7.48 ACSSPFFLEE10 pKa = 5.13 RR11 pKa = 11.84 ITDD14 pKa = 3.47 YY15 pKa = 10.75 RR16 pKa = 11.84 GRR18 pKa = 11.84 PVPLPCQKK26 pKa = 10.48 CNCCRR31 pKa = 11.84 ADD33 pKa = 3.12 KK34 pKa = 8.35 TTYY37 pKa = 5.55 WTRR40 pKa = 11.84 RR41 pKa = 11.84 ANYY44 pKa = 7.99 EE45 pKa = 3.66 WTHH48 pKa = 5.57 SVSSAFVTFTYY59 pKa = 10.51 DD60 pKa = 3.98 EE61 pKa = 4.06 EE62 pKa = 4.28 HH63 pKa = 6.63 LPIGGSLEE71 pKa = 3.89 PTLRR75 pKa = 11.84 RR76 pKa = 11.84 DD77 pKa = 3.48 DD78 pKa = 3.47 LHH80 pKa = 8.58 RR81 pKa = 11.84 YY82 pKa = 8.8 IDD84 pKa = 3.93 TVRR87 pKa = 11.84 HH88 pKa = 5.62 RR89 pKa = 11.84 LKK91 pKa = 10.55 KK92 pKa = 10.2 DD93 pKa = 3.0 GKK95 pKa = 9.74 IPPRR99 pKa = 11.84 CVPNFSYY106 pKa = 10.0 MASGEE111 pKa = 4.22 YY112 pKa = 10.24 GDD114 pKa = 4.35 SFGRR118 pKa = 11.84 PHH120 pKa = 5.56 YY121 pKa = 10.23 HH122 pKa = 5.88 VLFFGLDD129 pKa = 3.34 FEE131 pKa = 4.83 TCKK134 pKa = 10.69 KK135 pKa = 9.93 YY136 pKa = 10.81 LKK138 pKa = 10.48 KK139 pKa = 10.1 SWPYY143 pKa = 11.12 GSIAVNPVRR152 pKa = 11.84 DD153 pKa = 3.36 GAIRR157 pKa = 11.84 YY158 pKa = 6.56 VVSYY162 pKa = 10.45 ISKK165 pKa = 9.33 QLYY168 pKa = 9.89 GEE170 pKa = 4.16 EE171 pKa = 3.56 RR172 pKa = 11.84 DD173 pKa = 4.47 ARR175 pKa = 11.84 FFDD178 pKa = 4.3 YY179 pKa = 10.55 GVEE182 pKa = 4.1 PPFLSVSRR190 pKa = 11.84 GFGSGLFKK198 pKa = 10.98 DD199 pKa = 3.44 QAEE202 pKa = 4.51 NIRR205 pKa = 11.84 EE206 pKa = 3.92 YY207 pKa = 11.27 GALKK211 pKa = 10.35 FGQKK215 pKa = 9.8 FFSVPSYY222 pKa = 9.88 WKK224 pKa = 10.44 NKK226 pKa = 9.04 YY227 pKa = 9.88 IDD229 pKa = 3.65 MDD231 pKa = 3.53 IEE233 pKa = 4.34 KK234 pKa = 10.46 ADD236 pKa = 3.54 EE237 pKa = 4.41 RR238 pKa = 11.84 YY239 pKa = 10.14 EE240 pKa = 3.77 RR241 pKa = 11.84 AQRR244 pKa = 11.84 LRR246 pKa = 11.84 FEE248 pKa = 4.23 RR249 pKa = 11.84 WNLSGRR255 pKa = 11.84 HH256 pKa = 4.65 FQSPAGWTRR265 pKa = 11.84 EE266 pKa = 3.82 RR267 pKa = 11.84 NRR269 pKa = 11.84 VRR271 pKa = 11.84 EE272 pKa = 3.85 LVLYY276 pKa = 10.1 QKK278 pKa = 10.85 ARR280 pKa = 11.84 NRR282 pKa = 11.84 NSKK285 pKa = 9.87 VGYY288 pKa = 8.26 EE289 pKa = 4.05 RR290 pKa = 11.84 PIEE293 pKa = 3.94 LRR295 pKa = 11.84 PTGVGASIARR305 pKa = 11.84 RR306 pKa = 11.84 LRR308 pKa = 3.55
Molecular weight: 36.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.885
IPC2_protein 9.121
IPC_protein 9.165
Toseland 9.472
ProMoST 9.458
Dawson 9.823
Bjellqvist 9.619
Wikipedia 10.043
Rodwell 9.955
Grimsley 9.926
Solomon 9.853
Lehninger 9.794
Nozaki 9.619
DTASelect 9.575
Thurlkill 9.633
EMBOSS 9.926
Sillero 9.765
Patrickios 4.698
IPC_peptide 9.838
IPC2_peptide 8.536
IPC2.peptide.svr19 7.953
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1677
86
668
279.5
31.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.977 ± 0.807
1.073 ± 0.55
7.036 ± 1.033
4.711 ± 0.835
4.949 ± 0.591
6.619 ± 0.619
1.67 ± 0.405
4.592 ± 0.457
4.055 ± 1.028
8.229 ± 0.423
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.087 ± 0.248
4.114 ± 0.588
4.949 ± 1.04
4.114 ± 0.703
6.559 ± 1.084
9.064 ± 0.749
6.082 ± 0.81
6.798 ± 0.586
1.491 ± 0.267
4.83 ± 0.915
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here