Rhodococcus phage ReqiPoco6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pepyhexavirus; Rhodococcus virus Poco6

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 107 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4P7P3|D4P7P3_9CAUD Gp025 OS=Rhodococcus phage ReqiPoco6 OX=691964 GN=Poco6gene025 PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 6.68DD3 pKa = 4.0YY4 pKa = 11.36MLSTSDD10 pKa = 4.38NPFNPYY16 pKa = 7.55TQWEE20 pKa = 3.85QWYY23 pKa = 9.84AFDD26 pKa = 4.97AAAGYY31 pKa = 7.53HH32 pKa = 5.21TPAYY36 pKa = 9.62LARR39 pKa = 11.84IVVTSHH45 pKa = 6.18EE46 pKa = 4.36LSEE49 pKa = 4.47ADD51 pKa = 3.11QLLAIKK57 pKa = 10.25EE58 pKa = 4.53GIDD61 pKa = 3.83EE62 pKa = 4.18ILAMNLTGNYY72 pKa = 8.59IKK74 pKa = 11.01VMNPAAA80 pKa = 4.31

Molecular weight: 9.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4P7T1|D4P7T1_9CAUD Gp063 OS=Rhodococcus phage ReqiPoco6 OX=691964 GN=Poco6gene063 PE=4 SV=1
MM1 pKa = 7.63TDD3 pKa = 3.49AEE5 pKa = 4.29PPIVGTAVTLEE16 pKa = 4.16DD17 pKa = 3.51KK18 pKa = 10.8EE19 pKa = 4.2RR20 pKa = 11.84WRR22 pKa = 11.84KK23 pKa = 6.82NHH25 pKa = 5.94KK26 pKa = 10.12NKK28 pKa = 10.25LAIRR32 pKa = 11.84KK33 pKa = 7.32MNRR36 pKa = 11.84KK37 pKa = 8.3MINGRR42 pKa = 11.84LTSTLLQEE50 pKa = 4.83HH51 pKa = 6.65GTPSGYY57 pKa = 11.0NNWFCRR63 pKa = 11.84CIPCTQAYY71 pKa = 9.84NGRR74 pKa = 11.84KK75 pKa = 8.84RR76 pKa = 11.84DD77 pKa = 3.53NRR79 pKa = 11.84KK80 pKa = 9.1KK81 pKa = 10.35RR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 3.55

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

107

0

107

24467

35

1951

228.7

25.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.53 ± 0.42

0.572 ± 0.088

6.229 ± 0.256

6.253 ± 0.298

3.678 ± 0.202

7.594 ± 0.486

1.966 ± 0.202

6.0 ± 0.244

6.029 ± 0.307

8.097 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.714 ± 0.148

4.835 ± 0.198

4.189 ± 0.214

3.114 ± 0.155

5.211 ± 0.297

6.613 ± 0.215

6.45 ± 0.237

7.275 ± 0.213

1.328 ± 0.099

3.323 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski