Amino acid dipepetide frequency for Spiroplasma melliferum KC3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.531AlaAla: 3.531 ± 0.116
0.544AlaCys: 0.544 ± 0.037
2.407AlaAsp: 2.407 ± 0.084
3.001AlaGlu: 3.001 ± 0.101
2.605AlaPhe: 2.605 ± 0.089
3.405AlaGly: 3.405 ± 0.127
0.887AlaHis: 0.887 ± 0.049
5.915AlaIle: 5.915 ± 0.141
5.285AlaLys: 5.285 ± 0.142
5.525AlaLeu: 5.525 ± 0.129
1.439AlaMet: 1.439 ± 0.068
3.444AlaAsn: 3.444 ± 0.106
1.509AlaPro: 1.509 ± 0.07
2.128AlaGln: 2.128 ± 0.072
1.849AlaArg: 1.849 ± 0.082
2.884AlaSer: 2.884 ± 0.1
3.5AlaThr: 3.5 ± 0.106
3.472AlaVal: 3.472 ± 0.12
0.583AlaTrp: 0.583 ± 0.037
1.986AlaTyr: 1.986 ± 0.076
0.003AlaXaa: 0.003 ± 0.003
Cys
0.421CysAla: 0.421 ± 0.036
0.078CysCys: 0.078 ± 0.013
0.435CysAsp: 0.435 ± 0.036
0.435CysGlu: 0.435 ± 0.04
0.499CysPhe: 0.499 ± 0.04
0.463CysGly: 0.463 ± 0.044
0.22CysHis: 0.22 ± 0.026
0.533CysIle: 0.533 ± 0.04
0.488CysLys: 0.488 ± 0.042
0.892CysLeu: 0.892 ± 0.046
0.106CysMet: 0.106 ± 0.016
0.349CysAsn: 0.349 ± 0.03
0.223CysPro: 0.223 ± 0.026
0.354CysGln: 0.354 ± 0.034
0.19CysArg: 0.19 ± 0.025
0.499CysSer: 0.499 ± 0.038
0.318CysThr: 0.318 ± 0.03
0.332CysVal: 0.332 ± 0.033
0.125CysTrp: 0.125 ± 0.02
0.396CysTyr: 0.396 ± 0.035
0.0CysXaa: 0.0 ± 0.0
Asp
2.237AspAla: 2.237 ± 0.081
0.321AspCys: 0.321 ± 0.029
2.761AspAsp: 2.761 ± 0.107
3.533AspGlu: 3.533 ± 0.123
2.917AspPhe: 2.917 ± 0.094
2.449AspGly: 2.449 ± 0.105
0.728AspHis: 0.728 ± 0.045
4.671AspIle: 4.671 ± 0.133
4.351AspLys: 4.351 ± 0.121
5.469AspLeu: 5.469 ± 0.114
0.906AspMet: 0.906 ± 0.049
3.907AspAsn: 3.907 ± 0.128
1.559AspPro: 1.559 ± 0.071
1.849AspGln: 1.849 ± 0.073
1.344AspArg: 1.344 ± 0.063
2.482AspSer: 2.482 ± 0.093
2.094AspThr: 2.094 ± 0.075
2.948AspVal: 2.948 ± 0.103
0.647AspTrp: 0.647 ± 0.045
2.502AspTyr: 2.502 ± 0.089
0.0AspXaa: 0.0 ± 0.0
Glu
2.914GluAla: 2.914 ± 0.118
0.379GluCys: 0.379 ± 0.038
2.186GluAsp: 2.186 ± 0.089
3.651GluGlu: 3.651 ± 0.138
2.541GluPhe: 2.541 ± 0.074
2.351GluGly: 2.351 ± 0.09
1.065GluHis: 1.065 ± 0.054
6.247GluIle: 6.247 ± 0.165
5.87GluLys: 5.87 ± 0.14
6.205GluLeu: 6.205 ± 0.157
1.431GluMet: 1.431 ± 0.06
4.164GluAsn: 4.164 ± 0.114
1.506GluPro: 1.506 ± 0.065
3.321GluGln: 3.321 ± 0.104
2.069GluArg: 2.069 ± 0.088
2.295GluSer: 2.295 ± 0.09
3.361GluThr: 3.361 ± 0.104
3.31GluVal: 3.31 ± 0.113
0.591GluTrp: 0.591 ± 0.039
2.635GluTyr: 2.635 ± 0.084
0.0GluXaa: 0.0 ± 0.0
Phe
3.101PheAla: 3.101 ± 0.101
0.477PheCys: 0.477 ± 0.04
2.819PheAsp: 2.819 ± 0.102
2.63PheGlu: 2.63 ± 0.084
2.914PhePhe: 2.914 ± 0.116
2.872PheGly: 2.872 ± 0.118
0.747PheHis: 0.747 ± 0.043
5.09PheIle: 5.09 ± 0.159
4.192PheLys: 4.192 ± 0.122
5.53PheLeu: 5.53 ± 0.173
1.18PheMet: 1.18 ± 0.06
4.03PheAsn: 4.03 ± 0.134
1.311PhePro: 1.311 ± 0.068
1.754PheGln: 1.754 ± 0.073
1.353PheArg: 1.353 ± 0.067
3.637PheSer: 3.637 ± 0.103
2.789PheThr: 2.789 ± 0.103
2.906PheVal: 2.906 ± 0.102
0.711PheTrp: 0.711 ± 0.041
2.17PheTyr: 2.17 ± 0.074
0.0PheXaa: 0.0 ± 0.0
Gly
2.898GlyAla: 2.898 ± 0.099
0.374GlyCys: 0.374 ± 0.039
2.147GlyAsp: 2.147 ± 0.079
2.666GlyGlu: 2.666 ± 0.093
2.962GlyPhe: 2.962 ± 0.097
2.903GlyGly: 2.903 ± 0.122
0.971GlyHis: 0.971 ± 0.054
5.522GlyIle: 5.522 ± 0.146
4.376GlyLys: 4.376 ± 0.131
4.942GlyLeu: 4.942 ± 0.135
1.461GlyMet: 1.461 ± 0.076
2.75GlyAsn: 2.75 ± 0.096
1.141GlyPro: 1.141 ± 0.064
1.866GlyGln: 1.866 ± 0.089
1.581GlyArg: 1.581 ± 0.074
2.85GlySer: 2.85 ± 0.097
3.349GlyThr: 3.349 ± 0.102
3.163GlyVal: 3.163 ± 0.1
0.686GlyTrp: 0.686 ± 0.058
2.697GlyTyr: 2.697 ± 0.11
0.0GlyXaa: 0.0 ± 0.0
His
0.817HisAla: 0.817 ± 0.044
0.165HisCys: 0.165 ± 0.025
0.784HisAsp: 0.784 ± 0.046
0.773HisGlu: 0.773 ± 0.046
0.957HisPhe: 0.957 ± 0.052
0.848HisGly: 0.848 ± 0.048
0.519HisHis: 0.519 ± 0.043
1.244HisIle: 1.244 ± 0.065
1.113HisLys: 1.113 ± 0.053
1.829HisLeu: 1.829 ± 0.077
0.284HisMet: 0.284 ± 0.032
1.157HisAsn: 1.157 ± 0.057
0.686HisPro: 0.686 ± 0.056
1.012HisGln: 1.012 ± 0.057
0.63HisArg: 0.63 ± 0.038
0.848HisSer: 0.848 ± 0.054
0.725HisThr: 0.725 ± 0.048
0.745HisVal: 0.745 ± 0.045
0.24HisTrp: 0.24 ± 0.026
0.878HisTyr: 0.878 ± 0.053
0.0HisXaa: 0.0 ± 0.0
Ile
5.99IleAla: 5.99 ± 0.136
0.906IleCys: 0.906 ± 0.055
5.106IleAsp: 5.106 ± 0.145
4.836IleGlu: 4.836 ± 0.132
5.215IlePhe: 5.215 ± 0.152
5.19IleGly: 5.19 ± 0.132
1.224IleHis: 1.224 ± 0.058
10.243IleIle: 10.243 ± 0.21
8.587IleLys: 8.587 ± 0.191
8.737IleLeu: 8.737 ± 0.178
2.078IleMet: 2.078 ± 0.073
7.926IleAsn: 7.926 ± 0.17
3.115IlePro: 3.115 ± 0.112
2.312IleGln: 2.312 ± 0.072
2.783IleArg: 2.783 ± 0.093
5.742IleSer: 5.742 ± 0.134
5.787IleThr: 5.787 ± 0.132
5.764IleVal: 5.764 ± 0.128
1.23IleTrp: 1.23 ± 0.071
3.659IleTyr: 3.659 ± 0.096
0.0IleXaa: 0.0 ± 0.0
Lys
4.596LysAla: 4.596 ± 0.118
0.36LysCys: 0.36 ± 0.03
4.203LysAsp: 4.203 ± 0.116
6.308LysGlu: 6.308 ± 0.121
3.308LysPhe: 3.308 ± 0.115
3.508LysGly: 3.508 ± 0.1
1.45LysHis: 1.45 ± 0.067
9.086LysIle: 9.086 ± 0.186
8.721LysLys: 8.721 ± 0.174
7.837LysLeu: 7.837 ± 0.172
2.471LysMet: 2.471 ± 0.09
7.137LysAsn: 7.137 ± 0.161
2.845LysPro: 2.845 ± 0.095
3.902LysGln: 3.902 ± 0.124
2.661LysArg: 2.661 ± 0.096
3.854LysSer: 3.854 ± 0.108
5.856LysThr: 5.856 ± 0.145
4.925LysVal: 4.925 ± 0.14
1.143LysTrp: 1.143 ± 0.057
4.373LysTyr: 4.373 ± 0.112
0.0LysXaa: 0.0 ± 0.0
Leu
6.604LeuAla: 6.604 ± 0.146
0.664LeuCys: 0.664 ± 0.052
4.602LeuAsp: 4.602 ± 0.117
5.725LeuGlu: 5.725 ± 0.165
5.232LeuPhe: 5.232 ± 0.161
5.324LeuGly: 5.324 ± 0.109
1.274LeuHis: 1.274 ± 0.064
8.935LeuIle: 8.935 ± 0.191
9.036LeuLys: 9.036 ± 0.194
9.672LeuLeu: 9.672 ± 0.198
2.019LeuMet: 2.019 ± 0.073
6.671LeuAsn: 6.671 ± 0.151
3.171LeuPro: 3.171 ± 0.099
3.796LeuGln: 3.796 ± 0.103
2.931LeuArg: 2.931 ± 0.097
6.155LeuSer: 6.155 ± 0.149
6.994LeuThr: 6.994 ± 0.154
6.071LeuVal: 6.071 ± 0.136
1.107LeuTrp: 1.107 ± 0.057
3.419LeuTyr: 3.419 ± 0.107
0.0LeuXaa: 0.0 ± 0.0
Met
1.59MetAla: 1.59 ± 0.069
0.137MetCys: 0.137 ± 0.022
0.867MetAsp: 0.867 ± 0.049
0.973MetGlu: 0.973 ± 0.053
1.155MetPhe: 1.155 ± 0.062
1.113MetGly: 1.113 ± 0.063
0.273MetHis: 0.273 ± 0.028
2.016MetIle: 2.016 ± 0.071
2.072MetLys: 2.072 ± 0.075
2.379MetLeu: 2.379 ± 0.084
0.577MetMet: 0.577 ± 0.046
1.364MetAsn: 1.364 ± 0.066
0.731MetPro: 0.731 ± 0.047
0.998MetGln: 0.998 ± 0.058
0.725MetArg: 0.725 ± 0.049
1.129MetSer: 1.129 ± 0.053
1.277MetThr: 1.277 ± 0.055
1.361MetVal: 1.361 ± 0.058
0.218MetTrp: 0.218 ± 0.026
0.711MetTyr: 0.711 ± 0.04
0.0MetXaa: 0.0 ± 0.0
Asn
3.149AsnAla: 3.149 ± 0.107
0.505AsnCys: 0.505 ± 0.038
4.133AsnAsp: 4.133 ± 0.119
4.462AsnGlu: 4.462 ± 0.118
3.98AsnPhe: 3.98 ± 0.132
3.386AsnGly: 3.386 ± 0.115
1.185AsnHis: 1.185 ± 0.055
6.883AsnIle: 6.883 ± 0.145
6.523AsnLys: 6.523 ± 0.174
7.41AsnLeu: 7.41 ± 0.165
1.235AsnMet: 1.235 ± 0.061
7.217AsnAsn: 7.217 ± 0.187
2.532AsnPro: 2.532 ± 0.094
3.157AsnGln: 3.157 ± 0.099
2.125AsnArg: 2.125 ± 0.073
4.033AsnSer: 4.033 ± 0.139
3.229AsnThr: 3.229 ± 0.117
3.787AsnVal: 3.787 ± 0.109
1.082AsnTrp: 1.082 ± 0.065
3.941AsnTyr: 3.941 ± 0.129
0.0AsnXaa: 0.0 ± 0.0
Pro
1.514ProAla: 1.514 ± 0.069
0.165ProCys: 0.165 ± 0.022
1.573ProAsp: 1.573 ± 0.074
1.838ProGlu: 1.838 ± 0.069
1.629ProPhe: 1.629 ± 0.062
1.578ProGly: 1.578 ± 0.077
0.588ProHis: 0.588 ± 0.037
2.889ProIle: 2.889 ± 0.081
2.153ProLys: 2.153 ± 0.079
2.8ProLeu: 2.8 ± 0.087
0.577ProMet: 0.577 ± 0.047
2.334ProAsn: 2.334 ± 0.088
0.89ProPro: 0.89 ± 0.053
1.196ProGln: 1.196 ± 0.063
0.859ProArg: 0.859 ± 0.043
1.763ProSer: 1.763 ± 0.07
2.231ProThr: 2.231 ± 0.096
2.094ProVal: 2.094 ± 0.079
0.363ProTrp: 0.363 ± 0.034
1.11ProTyr: 1.11 ± 0.064
0.0ProXaa: 0.0 ± 0.0
Gln
2.329GlnAla: 2.329 ± 0.089
0.226GlnCys: 0.226 ± 0.027
1.757GlnAsp: 1.757 ± 0.079
2.691GlnGlu: 2.691 ± 0.095
1.838GlnPhe: 1.838 ± 0.073
1.788GlnGly: 1.788 ± 0.075
0.759GlnHis: 0.759 ± 0.049
3.729GlnIle: 3.729 ± 0.113
3.91GlnLys: 3.91 ± 0.1
4.256GlnLeu: 4.256 ± 0.125
0.795GlnMet: 0.795 ± 0.053
2.976GlnAsn: 2.976 ± 0.095
1.16GlnPro: 1.16 ± 0.069
2.582GlnGln: 2.582 ± 0.117
1.305GlnArg: 1.305 ± 0.067
1.67GlnSer: 1.67 ± 0.077
2.379GlnThr: 2.379 ± 0.09
2.058GlnVal: 2.058 ± 0.083
0.527GlnTrp: 0.527 ± 0.039
1.796GlnTyr: 1.796 ± 0.093
0.0GlnXaa: 0.0 ± 0.0
Arg
1.62ArgAla: 1.62 ± 0.071
0.204ArgCys: 0.204 ± 0.025
1.618ArgAsp: 1.618 ± 0.076
1.969ArgGlu: 1.969 ± 0.079
1.514ArgPhe: 1.514 ± 0.074
1.562ArgGly: 1.562 ± 0.072
0.633ArgHis: 0.633 ± 0.037
2.797ArgIle: 2.797 ± 0.094
2.741ArgLys: 2.741 ± 0.104
2.859ArgLeu: 2.859 ± 0.083
0.798ArgMet: 0.798 ± 0.051
2.014ArgAsn: 2.014 ± 0.077
0.873ArgPro: 0.873 ± 0.048
1.339ArgGln: 1.339 ± 0.063
1.311ArgArg: 1.311 ± 0.068
1.57ArgSer: 1.57 ± 0.063
1.604ArgThr: 1.604 ± 0.063
1.698ArgVal: 1.698 ± 0.076
0.455ArgTrp: 0.455 ± 0.038
1.439ArgTyr: 1.439 ± 0.06
0.0ArgXaa: 0.0 ± 0.0
Ser
2.831SerAla: 2.831 ± 0.096
0.463SerCys: 0.463 ± 0.033
2.599SerAsp: 2.599 ± 0.081
3.319SerGlu: 3.319 ± 0.107
3.419SerPhe: 3.419 ± 0.123
3.09SerGly: 3.09 ± 0.103
0.904SerHis: 0.904 ± 0.051
4.582SerIle: 4.582 ± 0.12
4.526SerLys: 4.526 ± 0.128
5.778SerLeu: 5.778 ± 0.143
0.959SerMet: 0.959 ± 0.048
3.941SerAsn: 3.941 ± 0.122
1.464SerPro: 1.464 ± 0.067
2.198SerGln: 2.198 ± 0.079
1.548SerArg: 1.548 ± 0.074
3.614SerSer: 3.614 ± 0.127
3.179SerThr: 3.179 ± 0.104
3.115SerVal: 3.115 ± 0.094
0.68SerTrp: 0.68 ± 0.045
2.669SerTyr: 2.669 ± 0.089
0.0SerXaa: 0.0 ± 0.0
Thr
3.458ThrAla: 3.458 ± 0.124
0.329ThrCys: 0.329 ± 0.034
2.976ThrAsp: 2.976 ± 0.097
2.951ThrGlu: 2.951 ± 0.101
2.906ThrPhe: 2.906 ± 0.091
3.466ThrGly: 3.466 ± 0.1
0.756ThrHis: 0.756 ± 0.046
6.105ThrIle: 6.105 ± 0.12
5.343ThrLys: 5.343 ± 0.126
5.672ThrLeu: 5.672 ± 0.153
1.23ThrMet: 1.23 ± 0.056
4.323ThrAsn: 4.323 ± 0.123
2.083ThrPro: 2.083 ± 0.073
1.604ThrGln: 1.604 ± 0.067
1.553ThrArg: 1.553 ± 0.071
3.321ThrSer: 3.321 ± 0.107
3.966ThrThr: 3.966 ± 0.121
3.561ThrVal: 3.561 ± 0.112
0.739ThrTrp: 0.739 ± 0.054
2.284ThrTyr: 2.284 ± 0.078
0.0ThrXaa: 0.0 ± 0.0
Val
3.57ValAla: 3.57 ± 0.112
0.527ValCys: 0.527 ± 0.035
3.079ValAsp: 3.079 ± 0.114
3.305ValGlu: 3.305 ± 0.095
2.786ValPhe: 2.786 ± 0.097
3.338ValGly: 3.338 ± 0.102
0.812ValHis: 0.812 ± 0.049
5.728ValIle: 5.728 ± 0.136
5.056ValLys: 5.056 ± 0.128
5.742ValLeu: 5.742 ± 0.146
1.088ValMet: 1.088 ± 0.058
3.888ValAsn: 3.888 ± 0.119
1.782ValPro: 1.782 ± 0.079
1.855ValGln: 1.855 ± 0.066
1.799ValArg: 1.799 ± 0.073
3.4ValSer: 3.4 ± 0.096
3.494ValThr: 3.494 ± 0.116
3.592ValVal: 3.592 ± 0.096
0.745ValTrp: 0.745 ± 0.048
2.128ValTyr: 2.128 ± 0.077
0.0ValXaa: 0.0 ± 0.0
Trp
0.58TrpAla: 0.58 ± 0.046
0.095TrpCys: 0.095 ± 0.017
0.761TrpAsp: 0.761 ± 0.05
0.591TrpGlu: 0.591 ± 0.042
0.8TrpPhe: 0.8 ± 0.057
0.53TrpGly: 0.53 ± 0.042
0.215TrpHis: 0.215 ± 0.028
1.266TrpIle: 1.266 ± 0.064
1.294TrpLys: 1.294 ± 0.07
1.194TrpLeu: 1.194 ± 0.055
0.343TrpMet: 0.343 ± 0.028
1.068TrpAsn: 1.068 ± 0.051
0.29TrpPro: 0.29 ± 0.027
0.513TrpGln: 0.513 ± 0.041
0.346TrpArg: 0.346 ± 0.035
0.672TrpSer: 0.672 ± 0.045
0.675TrpThr: 0.675 ± 0.044
0.58TrpVal: 0.58 ± 0.042
0.31TrpTrp: 0.31 ± 0.032
0.686TrpTyr: 0.686 ± 0.048
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.147TyrAla: 2.147 ± 0.088
0.441TyrCys: 0.441 ± 0.033
2.861TyrAsp: 2.861 ± 0.103
2.437TyrGlu: 2.437 ± 0.088
3.037TyrPhe: 3.037 ± 0.107
2.142TyrGly: 2.142 ± 0.082
1.043TyrHis: 1.043 ± 0.058
2.951TyrIle: 2.951 ± 0.09
2.906TyrLys: 2.906 ± 0.096
4.674TyrLeu: 4.674 ± 0.128
0.686TyrMet: 0.686 ± 0.037
3.296TyrAsn: 3.296 ± 0.111
1.274TyrPro: 1.274 ± 0.068
2.794TyrGln: 2.794 ± 0.101
1.654TyrArg: 1.654 ± 0.066
2.46TyrSer: 2.46 ± 0.092
1.818TyrThr: 1.818 ± 0.08
2.228TyrVal: 2.228 ± 0.084
0.627TyrTrp: 0.627 ± 0.05
2.418TyrTyr: 2.418 ± 0.09
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.003XaaAla: 0.003 ± 0.003
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1154 proteins (358577 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski