Amino acid dipepetide frequency for Mycobacterium chubuense (strain NBB4)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.725AlaAla: 20.725 ± 0.182	1.021AlaCys: 1.021 ± 0.021	8.71AlaAsp: 8.71 ± 0.075	7.706AlaGlu: 7.706 ± 0.08	3.492AlaPhe: 3.492 ± 0.049	12.107AlaGly: 12.107 ± 0.094	2.672AlaHis: 2.672 ± 0.042	5.072AlaIle: 5.072 ± 0.054	2.867AlaLys: 2.867 ± 0.051	13.169AlaLeu: 13.169 ± 0.124	2.916AlaMet: 2.916 ± 0.045	2.431AlaAsn: 2.431 ± 0.041	6.791AlaPro: 6.791 ± 0.082	3.934AlaGln: 3.934 ± 0.053	8.901AlaArg: 8.901 ± 0.083	6.202AlaSer: 6.202 ± 0.065	7.416AlaThr: 7.416 ± 0.08	12.287AlaVal: 12.287 ± 0.098	1.661AlaTrp: 1.661 ± 0.034	2.365AlaTyr: 2.365 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.093CysAla: 1.093 ± 0.027	0.105CysCys: 0.105 ± 0.008	0.577CysAsp: 0.577 ± 0.018	0.419CysGlu: 0.419 ± 0.015	0.226CysPhe: 0.226 ± 0.011	0.958CysGly: 0.958 ± 0.023	0.199CysHis: 0.199 ± 0.01	0.262CysIle: 0.262 ± 0.013	0.126CysLys: 0.126 ± 0.01	0.668CysLeu: 0.668 ± 0.022	0.126CysMet: 0.126 ± 0.008	0.164CysAsn: 0.164 ± 0.01	0.502CysPro: 0.502 ± 0.019	0.209CysGln: 0.209 ± 0.012	0.651CysArg: 0.651 ± 0.018	0.504CysSer: 0.504 ± 0.016	0.467CysThr: 0.467 ± 0.015	0.703CysVal: 0.703 ± 0.022	0.134CysTrp: 0.134 ± 0.009	0.179CysTyr: 0.179 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.946AspAla: 7.946 ± 0.071	0.455AspCys: 0.455 ± 0.016	4.498AspAsp: 4.498 ± 0.061	4.073AspGlu: 4.073 ± 0.055	1.798AspPhe: 1.798 ± 0.033	5.991AspGly: 5.991 ± 0.073	1.453AspHis: 1.453 ± 0.031	2.526AspIle: 2.526 ± 0.042	1.32AspLys: 1.32 ± 0.03	5.955AspLeu: 5.955 ± 0.064	1.027AspMet: 1.027 ± 0.022	1.219AspAsn: 1.219 ± 0.028	4.377AspPro: 4.377 ± 0.054	1.701AspGln: 1.701 ± 0.032	4.754AspArg: 4.754 ± 0.053	2.914AspSer: 2.914 ± 0.045	3.45AspThr: 3.45 ± 0.053	5.589AspVal: 5.589 ± 0.065	0.992AspTrp: 0.992 ± 0.025	1.381AspTyr: 1.381 ± 0.027	0.0AspXaa: 0.0 ± 0.0
Glu	6.103GluAla: 6.103 ± 0.073	0.362GluCys: 0.362 ± 0.017	2.456GluAsp: 2.456 ± 0.043	2.401GluGlu: 2.401 ± 0.043	1.751GluPhe: 1.751 ± 0.027	3.31GluGly: 3.31 ± 0.047	1.571GluHis: 1.571 ± 0.028	2.516GluIle: 2.516 ± 0.036	1.35GluLys: 1.35 ± 0.03	6.3GluLeu: 6.3 ± 0.071	1.047GluMet: 1.047 ± 0.021	1.084GluAsn: 1.084 ± 0.027	3.1GluPro: 3.1 ± 0.058	2.187GluGln: 2.187 ± 0.034	4.448GluArg: 4.448 ± 0.056	2.666GluSer: 2.666 ± 0.038	2.651GluThr: 2.651 ± 0.035	4.298GluVal: 4.298 ± 0.048	0.734GluTrp: 0.734 ± 0.02	1.079GluTyr: 1.079 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	3.994PheAla: 3.994 ± 0.047	0.332PheCys: 0.332 ± 0.013	2.461PheAsp: 2.461 ± 0.04	1.488PheGlu: 1.488 ± 0.03	0.969PhePhe: 0.969 ± 0.026	3.364PheGly: 3.364 ± 0.046	0.628PheHis: 0.628 ± 0.021	1.047PheIle: 1.047 ± 0.021	0.506PheLys: 0.506 ± 0.022	2.573PheLeu: 2.573 ± 0.039	0.477PheMet: 0.477 ± 0.017	0.707PheAsn: 0.707 ± 0.02	1.414PhePro: 1.414 ± 0.033	0.648PheGln: 0.648 ± 0.018	1.71PheArg: 1.71 ± 0.031	1.767PheSer: 1.767 ± 0.043	2.154PheThr: 2.154 ± 0.037	2.584PheVal: 2.584 ± 0.037	0.473PheTrp: 0.473 ± 0.017	0.686PheTyr: 0.686 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	10.474GlyAla: 10.474 ± 0.101	0.824GlyCys: 0.824 ± 0.022	5.171GlyAsp: 5.171 ± 0.053	4.414GlyGlu: 4.414 ± 0.047	2.978GlyPhe: 2.978 ± 0.039	8.017GlyGly: 8.017 ± 0.113	2.084GlyHis: 2.084 ± 0.036	3.928GlyIle: 3.928 ± 0.042	2.183GlyLys: 2.183 ± 0.036	8.393GlyLeu: 8.393 ± 0.075	2.138GlyMet: 2.138 ± 0.036	1.835GlyAsn: 1.835 ± 0.042	4.609GlyPro: 4.609 ± 0.064	2.699GlyGln: 2.699 ± 0.038	6.566GlyArg: 6.566 ± 0.065	5.238GlySer: 5.238 ± 0.073	5.386GlyThr: 5.386 ± 0.067	7.851GlyVal: 7.851 ± 0.071	1.629GlyTrp: 1.629 ± 0.032	2.347GlyTyr: 2.347 ± 0.036	0.0GlyXaa: 0.0 ± 0.0
His	2.508HisAla: 2.508 ± 0.041	0.216HisCys: 0.216 ± 0.01	1.409HisAsp: 1.409 ± 0.029	1.055HisGlu: 1.055 ± 0.023	0.709HisPhe: 0.709 ± 0.021	2.2HisGly: 2.2 ± 0.032	0.71HisHis: 0.71 ± 0.029	0.787HisIle: 0.787 ± 0.023	0.357HisLys: 0.357 ± 0.015	2.235HisLeu: 2.235 ± 0.042	0.37HisMet: 0.37 ± 0.014	0.475HisAsn: 0.475 ± 0.016	1.648HisPro: 1.648 ± 0.032	0.626HisGln: 0.626 ± 0.019	2.015HisArg: 2.015 ± 0.034	1.12HisSer: 1.12 ± 0.026	1.225HisThr: 1.225 ± 0.025	1.837HisVal: 1.837 ± 0.029	0.38HisTrp: 0.38 ± 0.015	0.546HisTyr: 0.546 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	6.254IleAla: 6.254 ± 0.059	0.369IleCys: 0.369 ± 0.015	3.321IleAsp: 3.321 ± 0.037	2.465IleGlu: 2.465 ± 0.037	0.977IlePhe: 0.977 ± 0.028	4.419IleGly: 4.419 ± 0.046	0.731IleHis: 0.731 ± 0.019	1.39IleIle: 1.39 ± 0.028	0.847IleLys: 0.847 ± 0.026	2.947IleLeu: 2.947 ± 0.044	0.591IleMet: 0.591 ± 0.018	1.051IleAsn: 1.051 ± 0.026	2.299IlePro: 2.299 ± 0.038	0.866IleGln: 0.866 ± 0.022	2.576IleArg: 2.576 ± 0.038	2.171IleSer: 2.171 ± 0.042	2.732IleThr: 2.732 ± 0.043	3.687IleVal: 3.687 ± 0.041	0.449IleTrp: 0.449 ± 0.019	0.742IleTyr: 0.742 ± 0.022	0.0IleXaa: 0.0 ± 0.0
Lys	2.642LysAla: 2.642 ± 0.05	0.123LysCys: 0.123 ± 0.009	1.08LysAsp: 1.08 ± 0.023	0.92LysGlu: 0.92 ± 0.027	0.586LysPhe: 0.586 ± 0.019	1.562LysGly: 1.562 ± 0.035	0.483LysHis: 0.483 ± 0.016	0.949LysIle: 0.949 ± 0.023	0.655LysLys: 0.655 ± 0.029	2.109LysLeu: 2.109 ± 0.043	0.466LysMet: 0.466 ± 0.019	0.509LysAsn: 0.509 ± 0.018	1.393LysPro: 1.393 ± 0.031	0.679LysGln: 0.679 ± 0.018	1.602LysArg: 1.602 ± 0.033	1.24LysSer: 1.24 ± 0.029	1.396LysThr: 1.396 ± 0.033	1.878LysVal: 1.878 ± 0.04	0.313LysTrp: 0.313 ± 0.013	0.447LysTyr: 0.447 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	14.094LeuAla: 14.094 ± 0.126	0.853LeuCys: 0.853 ± 0.021	6.487LeuAsp: 6.487 ± 0.072	4.347LeuGlu: 4.347 ± 0.06	2.67LeuPhe: 2.67 ± 0.048	8.576LeuGly: 8.576 ± 0.072	2.083LeuHis: 2.083 ± 0.039	4.051LeuIle: 4.051 ± 0.048	1.747LeuLys: 1.747 ± 0.034	9.521LeuLeu: 9.521 ± 0.097	1.774LeuMet: 1.774 ± 0.035	2.028LeuAsn: 2.028 ± 0.038	5.721LeuPro: 5.721 ± 0.056	2.524LeuGln: 2.524 ± 0.04	7.574LeuArg: 7.574 ± 0.072	5.707LeuSer: 5.707 ± 0.061	6.503LeuThr: 6.503 ± 0.061	8.236LeuVal: 8.236 ± 0.076	1.222LeuTrp: 1.222 ± 0.03	1.685LeuTyr: 1.685 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	2.65MetAla: 2.65 ± 0.036	0.194MetCys: 0.194 ± 0.011	0.896MetAsp: 0.896 ± 0.024	0.675MetGlu: 0.675 ± 0.02	0.605MetPhe: 0.605 ± 0.021	1.516MetGly: 1.516 ± 0.032	0.424MetHis: 0.424 ± 0.015	0.881MetIle: 0.881 ± 0.019	0.472MetLys: 0.472 ± 0.014	2.007MetLeu: 2.007 ± 0.033	0.431MetMet: 0.431 ± 0.016	0.504MetAsn: 0.504 ± 0.016	1.248MetPro: 1.248 ± 0.029	0.53MetGln: 0.53 ± 0.018	1.574MetArg: 1.574 ± 0.032	1.57MetSer: 1.57 ± 0.03	1.899MetThr: 1.899 ± 0.03	1.623MetVal: 1.623 ± 0.03	0.278MetTrp: 0.278 ± 0.013	0.338MetTyr: 0.338 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.644AsnAla: 2.644 ± 0.043	0.18AsnCys: 0.18 ± 0.011	1.214AsnAsp: 1.214 ± 0.029	0.906AsnGlu: 0.906 ± 0.022	0.645AsnPhe: 0.645 ± 0.019	1.968AsnGly: 1.968 ± 0.034	0.473AsnHis: 0.473 ± 0.019	0.859AsnIle: 0.859 ± 0.023	0.44AsnLys: 0.44 ± 0.015	1.945AsnLeu: 1.945 ± 0.035	0.4AsnMet: 0.4 ± 0.014	0.535AsnAsn: 0.535 ± 0.022	1.69AsnPro: 1.69 ± 0.035	0.618AsnGln: 0.618 ± 0.021	1.56AsnArg: 1.56 ± 0.03	1.135AsnSer: 1.135 ± 0.028	1.306AsnThr: 1.306 ± 0.029	1.735AsnVal: 1.735 ± 0.026	0.384AsnTrp: 0.384 ± 0.015	0.526AsnTyr: 0.526 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	7.76ProAla: 7.76 ± 0.075	0.32ProCys: 0.32 ± 0.013	4.358ProAsp: 4.358 ± 0.06	3.527ProGlu: 3.527 ± 0.045	1.647ProPhe: 1.647 ± 0.027	5.663ProGly: 5.663 ± 0.073	1.287ProHis: 1.287 ± 0.029	2.033ProIle: 2.033 ± 0.041	1.235ProLys: 1.235 ± 0.026	4.958ProLeu: 4.958 ± 0.053	1.208ProMet: 1.208 ± 0.028	1.245ProAsn: 1.245 ± 0.027	3.978ProPro: 3.978 ± 0.077	1.824ProGln: 1.824 ± 0.033	3.636ProArg: 3.636 ± 0.047	3.209ProSer: 3.209 ± 0.045	3.516ProThr: 3.516 ± 0.056	5.241ProVal: 5.241 ± 0.053	0.864ProTrp: 0.864 ± 0.022	1.173ProTyr: 1.173 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.524GlnAla: 3.524 ± 0.05	0.225GlnCys: 0.225 ± 0.011	1.18GlnAsp: 1.18 ± 0.025	1.061GlnGlu: 1.061 ± 0.025	0.976GlnPhe: 0.976 ± 0.021	1.969GlnGly: 1.969 ± 0.033	0.71GlnHis: 0.71 ± 0.02	1.476GlnIle: 1.476 ± 0.029	0.655GlnLys: 0.655 ± 0.02	3.26GlnLeu: 3.26 ± 0.048	0.73GlnMet: 0.73 ± 0.019	0.614GlnAsn: 0.614 ± 0.02	1.814GlnPro: 1.814 ± 0.036	1.244GlnGln: 1.244 ± 0.031	2.741GlnArg: 2.741 ± 0.04	1.471GlnSer: 1.471 ± 0.032	1.657GlnThr: 1.657 ± 0.039	2.357GlnVal: 2.357 ± 0.035	0.584GlnTrp: 0.584 ± 0.016	0.643GlnTyr: 0.643 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	8.588ArgAla: 8.588 ± 0.087	0.677ArgCys: 0.677 ± 0.019	4.423ArgAsp: 4.423 ± 0.055	3.901ArgGlu: 3.901 ± 0.054	2.467ArgPhe: 2.467 ± 0.038	5.332ArgGly: 5.332 ± 0.053	1.918ArgHis: 1.918 ± 0.034	3.352ArgIle: 3.352 ± 0.047	1.564ArgLys: 1.564 ± 0.031	7.577ArgLeu: 7.577 ± 0.078	1.792ArgMet: 1.792 ± 0.028	1.595ArgAsn: 1.595 ± 0.029	4.129ArgPro: 4.129 ± 0.059	2.28ArgGln: 2.28 ± 0.034	7.025ArgArg: 7.025 ± 0.088	4.163ArgSer: 4.163 ± 0.052	4.45ArgThr: 4.45 ± 0.046	5.619ArgVal: 5.619 ± 0.056	1.415ArgTrp: 1.415 ± 0.029	1.947ArgTyr: 1.947 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	7.344SerAla: 7.344 ± 0.084	0.426SerCys: 0.426 ± 0.016	3.336SerAsp: 3.336 ± 0.045	2.587SerGlu: 2.587 ± 0.036	1.627SerPhe: 1.627 ± 0.027	5.671SerGly: 5.671 ± 0.056	1.094SerHis: 1.094 ± 0.023	2.073SerIle: 2.073 ± 0.031	1.106SerLys: 1.106 ± 0.026	4.861SerLeu: 4.861 ± 0.047	1.32SerMet: 1.32 ± 0.023	1.075SerAsn: 1.075 ± 0.026	3.214SerPro: 3.214 ± 0.054	1.486SerGln: 1.486 ± 0.032	3.901SerArg: 3.901 ± 0.052	3.261SerSer: 3.261 ± 0.059	3.394SerThr: 3.394 ± 0.051	4.715SerVal: 4.715 ± 0.075	0.887SerTrp: 0.887 ± 0.026	1.19SerTyr: 1.19 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	8.211ThrAla: 8.211 ± 0.076	0.412ThrCys: 0.412 ± 0.015	3.824ThrAsp: 3.824 ± 0.052	3.132ThrGlu: 3.132 ± 0.044	1.932ThrPhe: 1.932 ± 0.041	6.031ThrGly: 6.031 ± 0.082	1.199ThrHis: 1.199 ± 0.028	2.394ThrIle: 2.394 ± 0.041	1.197ThrLys: 1.197 ± 0.03	5.795ThrLeu: 5.795 ± 0.075	1.226ThrMet: 1.226 ± 0.029	1.208ThrAsn: 1.208 ± 0.029	3.931ThrPro: 3.931 ± 0.057	1.532ThrGln: 1.532 ± 0.034	3.819ThrArg: 3.819 ± 0.049	3.32ThrSer: 3.32 ± 0.046	4.096ThrThr: 4.096 ± 0.069	6.25ThrVal: 6.25 ± 0.087	0.88ThrTrp: 0.88 ± 0.021	1.36ThrTyr: 1.36 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	11.89ValAla: 11.89 ± 0.088	0.786ValCys: 0.786 ± 0.02	5.827ValAsp: 5.827 ± 0.058	4.602ValGlu: 4.602 ± 0.052	2.634ValPhe: 2.634 ± 0.041	7.094ValGly: 7.094 ± 0.071	1.877ValHis: 1.877 ± 0.034	3.993ValIle: 3.993 ± 0.052	1.699ValLys: 1.699 ± 0.037	9.082ValLeu: 9.082 ± 0.081	1.634ValMet: 1.634 ± 0.029	1.999ValAsn: 1.999 ± 0.034	4.755ValPro: 4.755 ± 0.05	2.042ValGln: 2.042 ± 0.031	5.99ValArg: 5.99 ± 0.059	4.815ValSer: 4.815 ± 0.061	5.879ValThr: 5.879 ± 0.09	8.9ValVal: 8.9 ± 0.082	1.076ValTrp: 1.076 ± 0.028	1.624ValTyr: 1.624 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.598TrpAla: 1.598 ± 0.037	0.162TrpCys: 0.162 ± 0.009	0.823TrpAsp: 0.823 ± 0.025	0.65TrpGlu: 0.65 ± 0.019	0.534TrpPhe: 0.534 ± 0.018	1.026TrpGly: 1.026 ± 0.025	0.355TrpHis: 0.355 ± 0.014	0.651TrpIle: 0.651 ± 0.022	0.325TrpLys: 0.325 ± 0.014	1.742TrpLeu: 1.742 ± 0.035	0.35TrpMet: 0.35 ± 0.014	0.419TrpAsn: 0.419 ± 0.016	0.83TrpPro: 0.83 ± 0.023	0.611TrpGln: 0.611 ± 0.018	1.304TrpArg: 1.304 ± 0.026	0.933TrpSer: 0.933 ± 0.022	0.928TrpThr: 0.928 ± 0.023	1.134TrpVal: 1.134 ± 0.025	0.355TrpTrp: 0.355 ± 0.013	0.336TrpTyr: 0.336 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.402TyrAla: 2.402 ± 0.039	0.231TyrCys: 0.231 ± 0.011	1.37TyrAsp: 1.37 ± 0.028	1.087TyrGlu: 1.087 ± 0.025	0.761TyrPhe: 0.761 ± 0.021	1.993TyrGly: 1.993 ± 0.033	0.475TyrHis: 0.475 ± 0.017	0.644TyrIle: 0.644 ± 0.017	0.382TyrLys: 0.382 ± 0.014	2.302TyrLeu: 2.302 ± 0.038	0.292TyrMet: 0.292 ± 0.014	0.464TyrAsn: 0.464 ± 0.017	1.237TyrPro: 1.237 ± 0.027	0.646TyrGln: 0.646 ± 0.018	1.867TyrArg: 1.867 ± 0.035	1.162TyrSer: 1.162 ± 0.025	1.245TyrThr: 1.245 ± 0.034	1.678TyrVal: 1.678 ± 0.03	0.355TyrTrp: 0.355 ± 0.014	0.514TyrTyr: 0.514 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5779 proteins (1892690 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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