Mycobacterium virus Goose
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K0GAD7|K0GAD7_9CAUD Portal protein OS=Mycobacterium virus Goose OX=1211282 GN=12 PE=4 SV=1
MM1 pKa = 7.31 NPDD4 pKa = 4.31 DD5 pKa = 3.6 YY6 pKa = 11.31 TFAIRR11 pKa = 11.84 YY12 pKa = 7.46 EE13 pKa = 4.45 GQQEE17 pKa = 3.97 PDD19 pKa = 3.96 GPWVEE24 pKa = 4.48 MVAIQNDD31 pKa = 3.7 LAMAQAVWNDD41 pKa = 3.17 MAPYY45 pKa = 9.97 LEE47 pKa = 4.92 AMPNVRR53 pKa = 11.84 NAGIVFTPKK62 pKa = 10.23 INWQPYY68 pKa = 7.36 TGG70 pKa = 3.72
Molecular weight: 7.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.961
IPC_protein 3.808
Toseland 3.617
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.656
Grimsley 3.541
Solomon 3.77
Lehninger 3.732
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.935
Patrickios 1.901
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.838
Protein with the highest isoelectric point:
>tr|K0G8E0|K0G8E0_9CAUD Uncharacterized protein OS=Mycobacterium virus Goose OX=1211282 GN=86 PE=4 SV=1
MM1 pKa = 6.51 TTATMTRR8 pKa = 11.84 TMTLTQARR16 pKa = 11.84 EE17 pKa = 4.03 VTQDD21 pKa = 3.39 LLHH24 pKa = 5.74 EE25 pKa = 5.29 HH26 pKa = 6.47 GLKK29 pKa = 10.39 GWTVRR34 pKa = 11.84 FDD36 pKa = 3.42 NARR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 AGQCNYY47 pKa = 9.3 RR48 pKa = 11.84 DD49 pKa = 3.57 RR50 pKa = 11.84 VISLSRR56 pKa = 11.84 PLMALRR62 pKa = 11.84 SAEE65 pKa = 4.1 DD66 pKa = 3.37 TMQTITHH73 pKa = 7.22 EE74 pKa = 4.05 IAHH77 pKa = 6.83 AIVGHH82 pKa = 5.11 GHH84 pKa = 6.02 GHH86 pKa = 5.35 DD87 pKa = 3.52 QVWARR92 pKa = 11.84 KK93 pKa = 8.88 HH94 pKa = 6.47 RR95 pKa = 11.84 EE96 pKa = 3.5 LGGNGKK102 pKa = 9.5 RR103 pKa = 11.84 CFEE106 pKa = 4.46 MEE108 pKa = 5.32 AIDD111 pKa = 4.64 PTAPWVGTCDD121 pKa = 3.29 HH122 pKa = 6.9 GKK124 pKa = 9.51 QFARR128 pKa = 11.84 YY129 pKa = 7.47 RR130 pKa = 11.84 APKK133 pKa = 9.88 RR134 pKa = 11.84 LEE136 pKa = 3.58 GWRR139 pKa = 11.84 CRR141 pKa = 11.84 CRR143 pKa = 11.84 QGSSPVVWEE152 pKa = 3.91 KK153 pKa = 10.97 RR154 pKa = 11.84 RR155 pKa = 3.52
Molecular weight: 17.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.268
IPC_protein 9.721
Toseland 10.394
ProMoST 10.248
Dawson 10.496
Bjellqvist 10.218
Wikipedia 10.672
Rodwell 10.657
Grimsley 10.526
Solomon 10.599
Lehninger 10.57
Nozaki 10.452
DTASelect 10.175
Thurlkill 10.394
EMBOSS 10.789
Sillero 10.438
Patrickios 10.423
IPC_peptide 10.599
IPC2_peptide 9.516
IPC2.peptide.svr19 8.413
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
86
0
86
15929
27
909
185.2
20.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.957 ± 0.335
0.873 ± 0.123
6.422 ± 0.187
6.742 ± 0.269
3.057 ± 0.185
8.833 ± 0.498
2.04 ± 0.166
4.476 ± 0.176
4.225 ± 0.231
8.105 ± 0.266
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.329 ± 0.117
3.202 ± 0.169
6.234 ± 0.374
3.629 ± 0.187
6.887 ± 0.369
4.916 ± 0.236
5.751 ± 0.251
7.276 ± 0.187
2.109 ± 0.14
2.938 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here