Catellicoccus marimammalium M35/04/3
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1196 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K8Z9W2|K8Z9W2_9ENTE Uncharacterized protein OS=Catellicoccus marimammalium M35/04/3 OX=1234409 GN=C683_0466 PE=4 SV=1
MM1 pKa = 7.66 EE2 pKa = 5.85 FNPSAKK8 pKa = 9.54 IVFASLTGNTEE19 pKa = 3.81 EE20 pKa = 4.24 IAEE23 pKa = 4.62 VICDD27 pKa = 3.97 ALTDD31 pKa = 4.35 LNVDD35 pKa = 3.33 VTMANCDD42 pKa = 3.31 EE43 pKa = 5.38 AEE45 pKa = 4.16 ACDD48 pKa = 4.17 FQDD51 pKa = 3.65 VDD53 pKa = 3.54 ICVVATYY60 pKa = 9.26 TYY62 pKa = 11.39 GDD64 pKa = 3.61 GDD66 pKa = 4.31 LPDD69 pKa = 5.1 EE70 pKa = 4.45 IVDD73 pKa = 4.5 FYY75 pKa = 11.74 EE76 pKa = 4.92 DD77 pKa = 4.88 LADD80 pKa = 4.06 EE81 pKa = 4.94 DD82 pKa = 5.47 LDD84 pKa = 3.98 GKK86 pKa = 11.16 VYY88 pKa = 10.51 GVAGSGDD95 pKa = 3.19 TTYY98 pKa = 11.88 DD99 pKa = 3.47 HH100 pKa = 6.9 FCKK103 pKa = 10.5 SVEE106 pKa = 4.18 DD107 pKa = 4.38 FDD109 pKa = 5.26 AQFASTGATKK119 pKa = 10.35 GAEE122 pKa = 4.08 NVKK125 pKa = 10.4 VEE127 pKa = 4.57 LEE129 pKa = 4.2 PEE131 pKa = 4.36 AEE133 pKa = 4.91 DD134 pKa = 3.69 IQHH137 pKa = 6.83 LEE139 pKa = 3.99 AFAKK143 pKa = 10.57 SLVEE147 pKa = 4.1 KK148 pKa = 10.5 AQEE151 pKa = 3.71 LHH153 pKa = 6.17 EE154 pKa = 4.38 AA155 pKa = 3.96
Molecular weight: 16.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.643
IPC_protein 3.643
Toseland 3.427
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.554
Rodwell 3.465
Grimsley 3.338
Solomon 3.617
Lehninger 3.567
Nozaki 3.745
DTASelect 3.948
Thurlkill 3.478
EMBOSS 3.554
Sillero 3.757
Patrickios 0.731
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.712
Protein with the highest isoelectric point:
>tr|K8ZNM4|K8ZNM4_9ENTE LtrC-like protein OS=Catellicoccus marimammalium M35/04/3 OX=1234409 GN=C683_0853 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 9.16 RR10 pKa = 11.84 KK11 pKa = 9.12 RR12 pKa = 11.84 QKK14 pKa = 9.38 VHH16 pKa = 5.8 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 9.46 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.81 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.427
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.149
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1196
0
1196
382755
37
1952
320.0
36.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.725 ± 0.075
0.908 ± 0.022
5.122 ± 0.065
8.378 ± 0.102
4.206 ± 0.058
6.179 ± 0.079
2.176 ± 0.029
7.244 ± 0.074
7.79 ± 0.103
9.508 ± 0.09
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.732 ± 0.036
4.432 ± 0.059
3.515 ± 0.038
4.833 ± 0.073
3.816 ± 0.056
5.355 ± 0.045
5.493 ± 0.051
6.506 ± 0.059
1.078 ± 0.026
4.006 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here