Amino acid dipepetide frequency for Prevotella sp. CAG:617

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.427AlaAla: 7.427 ± 0.156	1.315AlaCys: 1.315 ± 0.05	5.046AlaAsp: 5.046 ± 0.101	5.006AlaGlu: 5.006 ± 0.099	3.162AlaPhe: 3.162 ± 0.077	5.757AlaGly: 5.757 ± 0.083	1.654AlaHis: 1.654 ± 0.06	4.288AlaIle: 4.288 ± 0.093	3.413AlaLys: 3.413 ± 0.083	7.786AlaLeu: 7.786 ± 0.13	2.052AlaMet: 2.052 ± 0.061	2.94AlaAsn: 2.94 ± 0.074	2.692AlaPro: 2.692 ± 0.071	3.399AlaGln: 3.399 ± 0.09	4.393AlaArg: 4.393 ± 0.091	4.293AlaSer: 4.293 ± 0.085	4.245AlaThr: 4.245 ± 0.085	5.328AlaVal: 5.328 ± 0.117	0.872AlaTrp: 0.872 ± 0.037	3.034AlaTyr: 3.034 ± 0.076	0.003AlaXaa: 0.003 ± 0.002
Cys	0.97CysAla: 0.97 ± 0.046	0.268CysCys: 0.268 ± 0.019	0.665CysAsp: 0.665 ± 0.031	0.633CysGlu: 0.633 ± 0.029	0.678CysPhe: 0.678 ± 0.03	1.268CysGly: 1.268 ± 0.04	0.414CysHis: 0.414 ± 0.025	0.938CysIle: 0.938 ± 0.039	0.616CysLys: 0.616 ± 0.031	1.372CysLeu: 1.372 ± 0.05	0.419CysMet: 0.419 ± 0.021	0.578CysAsn: 0.578 ± 0.033	0.699CysPro: 0.699 ± 0.036	0.534CysGln: 0.534 ± 0.029	0.976CysArg: 0.976 ± 0.042	0.898CysSer: 0.898 ± 0.04	0.739CysThr: 0.739 ± 0.034	1.006CysVal: 1.006 ± 0.04	0.179CysTrp: 0.179 ± 0.017	0.605CysTyr: 0.605 ± 0.028	0.0CysXaa: 0.0 ± 0.0
Asp	4.077AspAla: 4.077 ± 0.098	0.73AspCys: 0.73 ± 0.037	2.766AspAsp: 2.766 ± 0.082	3.78AspGlu: 3.78 ± 0.079	2.837AspPhe: 2.837 ± 0.064	4.111AspGly: 4.111 ± 0.089	1.07AspHis: 1.07 ± 0.044	3.595AspIle: 3.595 ± 0.076	2.896AspLys: 2.896 ± 0.081	4.778AspLeu: 4.778 ± 0.09	1.537AspMet: 1.537 ± 0.05	2.349AspAsn: 2.349 ± 0.056	1.871AspPro: 1.871 ± 0.055	1.377AspGln: 1.377 ± 0.045	2.66AspArg: 2.66 ± 0.068	3.225AspSer: 3.225 ± 0.066	2.762AspThr: 2.762 ± 0.077	3.69AspVal: 3.69 ± 0.08	0.739AspTrp: 0.739 ± 0.037	2.623AspTyr: 2.623 ± 0.066	0.002AspXaa: 0.002 ± 0.002
Glu	5.14GluAla: 5.14 ± 0.108	0.633GluCys: 0.633 ± 0.034	2.64GluAsp: 2.64 ± 0.064	4.025GluGlu: 4.025 ± 0.103	2.079GluPhe: 2.079 ± 0.056	3.911GluGly: 3.911 ± 0.082	1.331GluHis: 1.331 ± 0.047	3.929GluIle: 3.929 ± 0.097	4.37GluLys: 4.37 ± 0.094	5.392GluLeu: 5.392 ± 0.095	1.799GluMet: 1.799 ± 0.055	3.116GluAsn: 3.116 ± 0.08	1.845GluPro: 1.845 ± 0.056	3.087GluGln: 3.087 ± 0.076	3.368GluArg: 3.368 ± 0.072	2.959GluSer: 2.959 ± 0.069	2.98GluThr: 2.98 ± 0.083	3.921GluVal: 3.921 ± 0.096	0.769GluTrp: 0.769 ± 0.035	2.142GluTyr: 2.142 ± 0.058	0.003GluXaa: 0.003 ± 0.002
Phe	2.953PheAla: 2.953 ± 0.075	0.839PheCys: 0.839 ± 0.035	2.534PheAsp: 2.534 ± 0.067	2.289PheGlu: 2.289 ± 0.056	2.127PhePhe: 2.127 ± 0.068	3.156PheGly: 3.156 ± 0.071	0.944PheHis: 0.944 ± 0.04	2.604PheIle: 2.604 ± 0.074	2.166PheLys: 2.166 ± 0.073	3.941PheLeu: 3.941 ± 0.092	1.211PheMet: 1.211 ± 0.039	2.138PheAsn: 2.138 ± 0.061	1.554PhePro: 1.554 ± 0.049	1.402PheGln: 1.402 ± 0.04	2.212PheArg: 2.212 ± 0.063	3.333PheSer: 3.333 ± 0.087	2.507PheThr: 2.507 ± 0.063	3.017PheVal: 3.017 ± 0.079	0.484PheTrp: 0.484 ± 0.028	1.865PheTyr: 1.865 ± 0.053	0.003PheXaa: 0.003 ± 0.002
Gly	4.927GlyAla: 4.927 ± 0.086	1.127GlyCys: 1.127 ± 0.051	3.205GlyAsp: 3.205 ± 0.07	3.586GlyGlu: 3.586 ± 0.08	3.17GlyPhe: 3.17 ± 0.077	5.126GlyGly: 5.126 ± 0.109	1.699GlyHis: 1.699 ± 0.052	4.91GlyIle: 4.91 ± 0.089	4.439GlyLys: 4.439 ± 0.094	6.296GlyLeu: 6.296 ± 0.107	2.158GlyMet: 2.158 ± 0.055	3.156GlyAsn: 3.156 ± 0.083	1.682GlyPro: 1.682 ± 0.055	2.877GlyGln: 2.877 ± 0.077	3.998GlyArg: 3.998 ± 0.081	4.058GlySer: 4.058 ± 0.087	4.029GlyThr: 4.029 ± 0.082	5.053GlyVal: 5.053 ± 0.099	0.981GlyTrp: 0.981 ± 0.04	3.023GlyTyr: 3.023 ± 0.076	0.0GlyXaa: 0.0 ± 0.0
His	1.708HisAla: 1.708 ± 0.055	0.391HisCys: 0.391 ± 0.025	1.166HisAsp: 1.166 ± 0.047	1.228HisGlu: 1.228 ± 0.046	1.206HisPhe: 1.206 ± 0.044	1.508HisGly: 1.508 ± 0.054	0.69HisHis: 0.69 ± 0.038	1.557HisIle: 1.557 ± 0.051	1.064HisLys: 1.064 ± 0.038	2.236HisLeu: 2.236 ± 0.066	0.474HisMet: 0.474 ± 0.027	0.958HisAsn: 0.958 ± 0.04	1.238HisPro: 1.238 ± 0.053	0.847HisGln: 0.847 ± 0.041	1.217HisArg: 1.217 ± 0.054	1.106HisSer: 1.106 ± 0.041	1.338HisThr: 1.338 ± 0.047	1.5HisVal: 1.5 ± 0.053	0.285HisTrp: 0.285 ± 0.02	1.072HisTyr: 1.072 ± 0.041	0.0HisXaa: 0.0 ± 0.0
Ile	4.484IleAla: 4.484 ± 0.1	0.969IleCys: 0.969 ± 0.041	3.92IleAsp: 3.92 ± 0.071	3.835IleGlu: 3.835 ± 0.082	2.477IlePhe: 2.477 ± 0.065	4.223IleGly: 4.223 ± 0.092	1.362IleHis: 1.362 ± 0.047	3.926IleIle: 3.926 ± 0.106	3.612IleLys: 3.612 ± 0.08	5.394IleLeu: 5.394 ± 0.108	1.553IleMet: 1.553 ± 0.046	3.09IleAsn: 3.09 ± 0.082	2.843IlePro: 2.843 ± 0.075	2.07IleGln: 2.07 ± 0.058	3.208IleArg: 3.208 ± 0.073	4.211IleSer: 4.211 ± 0.078	3.515IleThr: 3.515 ± 0.08	4.299IleVal: 4.299 ± 0.098	0.588IleTrp: 0.588 ± 0.03	2.318IleTyr: 2.318 ± 0.061	0.0IleXaa: 0.0 ± 0.0
Lys	4.608LysAla: 4.608 ± 0.092	0.519LysCys: 0.519 ± 0.028	2.842LysAsp: 2.842 ± 0.067	3.726LysGlu: 3.726 ± 0.091	1.905LysPhe: 1.905 ± 0.049	3.61LysGly: 3.61 ± 0.081	1.106LysHis: 1.106 ± 0.049	3.683LysIle: 3.683 ± 0.079	3.981LysLys: 3.981 ± 0.095	4.684LysLeu: 4.684 ± 0.077	1.824LysMet: 1.824 ± 0.057	3.107LysAsn: 3.107 ± 0.076	1.959LysPro: 1.959 ± 0.055	2.392LysGln: 2.392 ± 0.065	2.859LysArg: 2.859 ± 0.077	2.985LysSer: 2.985 ± 0.077	3.04LysThr: 3.04 ± 0.066	3.599LysVal: 3.599 ± 0.083	0.695LysTrp: 0.695 ± 0.032	2.239LysTyr: 2.239 ± 0.07	0.002LysXaa: 0.002 ± 0.002
Leu	6.985LeuAla: 6.985 ± 0.121	1.574LeuCys: 1.574 ± 0.05	4.548LeuAsp: 4.548 ± 0.092	4.927LeuGlu: 4.927 ± 0.102	4.197LeuPhe: 4.197 ± 0.096	5.971LeuGly: 5.971 ± 0.129	2.358LeuHis: 2.358 ± 0.067	5.115LeuIle: 5.115 ± 0.101	5.449LeuLys: 5.449 ± 0.09	9.642LeuLeu: 9.642 ± 0.181	2.705LeuMet: 2.705 ± 0.065	4.399LeuAsn: 4.399 ± 0.092	4.625LeuPro: 4.625 ± 0.091	4.467LeuGln: 4.467 ± 0.087	5.294LeuArg: 5.294 ± 0.115	6.68LeuSer: 6.68 ± 0.112	6.045LeuThr: 6.045 ± 0.122	5.42LeuVal: 5.42 ± 0.116	1.129LeuTrp: 1.129 ± 0.048	3.632LeuTyr: 3.632 ± 0.079	0.002LeuXaa: 0.002 ± 0.001
Met	2.377MetAla: 2.377 ± 0.062	0.323MetCys: 0.323 ± 0.022	1.411MetAsp: 1.411 ± 0.055	1.602MetGlu: 1.602 ± 0.049	0.955MetPhe: 0.955 ± 0.041	1.859MetGly: 1.859 ± 0.059	0.514MetHis: 0.514 ± 0.024	1.415MetIle: 1.415 ± 0.052	2.067MetLys: 2.067 ± 0.059	2.703MetLeu: 2.703 ± 0.07	0.901MetMet: 0.901 ± 0.04	1.42MetAsn: 1.42 ± 0.047	1.368MetPro: 1.368 ± 0.043	1.265MetGln: 1.265 ± 0.052	1.497MetArg: 1.497 ± 0.052	1.72MetSer: 1.72 ± 0.058	1.532MetThr: 1.532 ± 0.047	1.736MetVal: 1.736 ± 0.053	0.266MetTrp: 0.266 ± 0.021	0.759MetTyr: 0.759 ± 0.035	0.002MetXaa: 0.002 ± 0.002
Asn	3.335AsnAla: 3.335 ± 0.08	0.579AsnCys: 0.579 ± 0.036	2.327AsnAsp: 2.327 ± 0.062	2.549AsnGlu: 2.549 ± 0.066	1.999AsnPhe: 1.999 ± 0.061	3.502AsnGly: 3.502 ± 0.088	0.973AsnHis: 0.973 ± 0.039	3.167AsnIle: 3.167 ± 0.08	2.45AsnLys: 2.45 ± 0.075	4.297AsnLeu: 4.297 ± 0.092	1.186AsnMet: 1.186 ± 0.039	2.278AsnAsn: 2.278 ± 0.066	2.258AsnPro: 2.258 ± 0.065	1.688AsnGln: 1.688 ± 0.05	2.518AsnArg: 2.518 ± 0.07	2.484AsnSer: 2.484 ± 0.072	2.484AsnThr: 2.484 ± 0.066	3.079AsnVal: 3.079 ± 0.071	0.627AsnTrp: 0.627 ± 0.035	2.035AsnTyr: 2.035 ± 0.057	0.002AsnXaa: 0.002 ± 0.001
Pro	3.375ProAla: 3.375 ± 0.079	0.465ProCys: 0.465 ± 0.025	2.856ProAsp: 2.856 ± 0.073	3.279ProGlu: 3.279 ± 0.069	1.751ProPhe: 1.751 ± 0.053	2.628ProGly: 2.628 ± 0.061	0.952ProHis: 0.952 ± 0.038	2.142ProIle: 2.142 ± 0.07	1.771ProLys: 1.771 ± 0.056	3.687ProLeu: 3.687 ± 0.079	1.003ProMet: 1.003 ± 0.034	1.549ProAsn: 1.549 ± 0.052	1.07ProPro: 1.07 ± 0.048	2.021ProGln: 2.021 ± 0.047	1.703ProArg: 1.703 ± 0.061	2.253ProSer: 2.253 ± 0.064	2.102ProThr: 2.102 ± 0.063	3.07ProVal: 3.07 ± 0.07	0.484ProTrp: 0.484 ± 0.03	1.784ProTyr: 1.784 ± 0.05	0.003ProXaa: 0.003 ± 0.002
Gln	3.456GlnAla: 3.456 ± 0.089	0.476GlnCys: 0.476 ± 0.027	1.617GlnAsp: 1.617 ± 0.057	2.373GlnGlu: 2.373 ± 0.064	1.465GlnPhe: 1.465 ± 0.043	2.389GlnGly: 2.389 ± 0.058	1.078GlnHis: 1.078 ± 0.048	2.606GlnIle: 2.606 ± 0.066	2.44GlnLys: 2.44 ± 0.061	4.396GlnLeu: 4.396 ± 0.11	1.268GlnMet: 1.268 ± 0.046	1.837GlnAsn: 1.837 ± 0.058	1.734GlnPro: 1.734 ± 0.053	2.452GlnGln: 2.452 ± 0.068	2.303GlnArg: 2.303 ± 0.064	2.136GlnSer: 2.136 ± 0.067	2.434GlnThr: 2.434 ± 0.065	2.491GlnVal: 2.491 ± 0.059	0.562GlnTrp: 0.562 ± 0.032	1.708GlnTyr: 1.708 ± 0.048	0.0GlnXaa: 0.0 ± 0.0
Arg	3.562ArgAla: 3.562 ± 0.091	0.641ArgCys: 0.641 ± 0.031	2.259ArgAsp: 2.259 ± 0.066	3.157ArgGlu: 3.157 ± 0.073	2.648ArgPhe: 2.648 ± 0.067	2.926ArgGly: 2.926 ± 0.061	1.611ArgHis: 1.611 ± 0.055	3.684ArgIle: 3.684 ± 0.076	3.282ArgLys: 3.282 ± 0.067	5.713ArgLeu: 5.713 ± 0.11	1.774ArgMet: 1.774 ± 0.054	2.447ArgAsn: 2.447 ± 0.06	2.236ArgPro: 2.236 ± 0.062	2.862ArgGln: 2.862 ± 0.08	3.804ArgArg: 3.804 ± 0.097	2.786ArgSer: 2.786 ± 0.069	2.854ArgThr: 2.854 ± 0.067	3.111ArgVal: 3.111 ± 0.083	0.676ArgTrp: 0.676 ± 0.032	2.446ArgTyr: 2.446 ± 0.071	0.005ArgXaa: 0.005 ± 0.003
Ser	4.557SerAla: 4.557 ± 0.092	0.873SerCys: 0.873 ± 0.039	3.238SerAsp: 3.238 ± 0.077	3.151SerGlu: 3.151 ± 0.069	2.886SerPhe: 2.886 ± 0.074	4.584SerGly: 4.584 ± 0.089	1.303SerHis: 1.303 ± 0.045	3.835SerIle: 3.835 ± 0.089	2.749SerLys: 2.749 ± 0.061	6.148SerLeu: 6.148 ± 0.118	1.545SerMet: 1.545 ± 0.05	2.421SerAsn: 2.421 ± 0.062	2.387SerPro: 2.387 ± 0.06	2.215SerGln: 2.215 ± 0.06	3.279SerArg: 3.279 ± 0.076	3.892SerSer: 3.892 ± 0.099	3.359SerThr: 3.359 ± 0.075	4.443SerVal: 4.443 ± 0.098	0.827SerTrp: 0.827 ± 0.042	2.544SerTyr: 2.544 ± 0.074	0.0SerXaa: 0.0 ± 0.0
Thr	4.699ThrAla: 4.699 ± 0.089	0.725ThrCys: 0.725 ± 0.03	3.69ThrAsp: 3.69 ± 0.083	3.131ThrGlu: 3.131 ± 0.069	2.54ThrPhe: 2.54 ± 0.062	4.285ThrGly: 4.285 ± 0.084	1.209ThrHis: 1.209 ± 0.046	3.311ThrIle: 3.311 ± 0.076	2.181ThrLys: 2.181 ± 0.063	5.702ThrLeu: 5.702 ± 0.102	1.046ThrMet: 1.046 ± 0.046	2.296ThrAsn: 2.296 ± 0.067	3.0ThrPro: 3.0 ± 0.068	2.053ThrGln: 2.053 ± 0.063	2.597ThrArg: 2.597 ± 0.055	3.267ThrSer: 3.267 ± 0.071	3.335ThrThr: 3.335 ± 0.082	3.871ThrVal: 3.871 ± 0.091	0.684ThrTrp: 0.684 ± 0.035	2.45ThrTyr: 2.45 ± 0.067	0.0ThrXaa: 0.0 ± 0.0
Val	5.332ValAla: 5.332 ± 0.107	1.224ValCys: 1.224 ± 0.043	3.726ValAsp: 3.726 ± 0.091	4.217ValGlu: 4.217 ± 0.09	2.797ValPhe: 2.797 ± 0.071	4.787ValGly: 4.787 ± 0.103	1.244ValHis: 1.244 ± 0.048	3.989ValIle: 3.989 ± 0.088	3.664ValLys: 3.664 ± 0.077	6.067ValLeu: 6.067 ± 0.117	1.822ValMet: 1.822 ± 0.063	2.928ValAsn: 2.928 ± 0.07	2.768ValPro: 2.768 ± 0.072	2.068ValGln: 2.068 ± 0.061	3.589ValArg: 3.589 ± 0.091	4.946ValSer: 4.946 ± 0.097	3.666ValThr: 3.666 ± 0.077	5.283ValVal: 5.283 ± 0.116	0.786ValTrp: 0.786 ± 0.039	2.563ValTyr: 2.563 ± 0.067	0.0ValXaa: 0.0 ± 0.0
Trp	0.836TrpAla: 0.836 ± 0.036	0.193TrpCys: 0.193 ± 0.017	0.571TrpAsp: 0.571 ± 0.032	0.618TrpGlu: 0.618 ± 0.032	0.484TrpPhe: 0.484 ± 0.029	0.93TrpGly: 0.93 ± 0.039	0.343TrpHis: 0.343 ± 0.022	0.784TrpIle: 0.784 ± 0.037	0.79TrpLys: 0.79 ± 0.037	1.281TrpLeu: 1.281 ± 0.047	0.413TrpMet: 0.413 ± 0.027	0.749TrpAsn: 0.749 ± 0.037	0.357TrpPro: 0.357 ± 0.022	0.562TrpGln: 0.562 ± 0.031	0.735TrpArg: 0.735 ± 0.033	0.692TrpSer: 0.692 ± 0.034	0.67TrpThr: 0.67 ± 0.038	0.678TrpVal: 0.678 ± 0.034	0.182TrpTrp: 0.182 ± 0.018	0.442TrpTyr: 0.442 ± 0.028	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.296TyrAla: 3.296 ± 0.077	0.587TyrCys: 0.587 ± 0.033	2.446TyrAsp: 2.446 ± 0.061	2.324TyrGlu: 2.324 ± 0.066	1.916TyrPhe: 1.916 ± 0.052	2.971TyrGly: 2.971 ± 0.084	0.933TyrHis: 0.933 ± 0.039	2.415TyrIle: 2.415 ± 0.06	1.962TyrLys: 1.962 ± 0.062	3.646TyrLeu: 3.646 ± 0.089	1.004TyrMet: 1.004 ± 0.037	2.061TyrAsn: 2.061 ± 0.068	1.759TyrPro: 1.759 ± 0.047	1.58TyrGln: 1.58 ± 0.05	2.272TyrArg: 2.272 ± 0.063	2.313TyrSer: 2.313 ± 0.068	2.434TyrThr: 2.434 ± 0.068	2.871TyrVal: 2.871 ± 0.079	0.494TyrTrp: 0.494 ± 0.029	1.947TyrTyr: 1.947 ± 0.063	0.002TyrXaa: 0.002 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.003XaaCys: 0.003 ± 0.002	0.002XaaAsp: 0.002 ± 0.001	0.003XaaGlu: 0.003 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.002XaaLys: 0.002 ± 0.002	0.002XaaLeu: 0.002 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.002XaaAsn: 0.002 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.003XaaGln: 0.003 ± 0.002	0.006XaaArg: 0.006 ± 0.003	0.002XaaSer: 0.002 ± 0.002	0.002XaaThr: 0.002 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.002XaaTrp: 0.002 ± 0.002	0.0XaaTyr: 0.0 ± 0.0	0.026XaaXaa: 0.026 ± 0.008

Statistics based on 1928 proteins (649267 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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