Novosphingobium malaysiense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4270 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B1ZJH1|A0A0B1ZJH1_9SPHN Uncharacterized protein OS=Novosphingobium malaysiense OX=1348853 GN=LK12_10185 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.17KK3 pKa = 10.08ISGLLLVGCSALALAGCGADD23 pKa = 5.0DD24 pKa = 4.29ISSPGAGSITINNPTPTPTPTPTPTSGVVTPAASCPSFNATGGLTDD70 pKa = 3.84SGTITGPEE78 pKa = 4.3GEE80 pKa = 4.38WRR82 pKa = 11.84VCTLPSLVDD91 pKa = 3.64VSSSLPKK98 pKa = 10.46VSGVLYY104 pKa = 10.17RR105 pKa = 11.84LNGRR109 pKa = 11.84VDD111 pKa = 3.49VGCDD115 pKa = 3.09GGFSAPTAAAPFATTTASCGGRR137 pKa = 11.84SLTADD142 pKa = 3.31TDD144 pKa = 3.71VTLTIDD150 pKa = 3.61PGVIVYY156 pKa = 9.83GEE158 pKa = 4.15NGAEE162 pKa = 4.11PAWLAVNRR170 pKa = 11.84GNKK173 pKa = 8.81INAVGTASQPIIFTSRR189 pKa = 11.84QNVVGTNDD197 pKa = 5.14DD198 pKa = 3.64SSQGQWGGVVLMGRR212 pKa = 11.84GIVTDD217 pKa = 3.95CAYY220 pKa = 10.72GSTAAGTCEE229 pKa = 3.94RR230 pKa = 11.84DD231 pKa = 3.55TEE233 pKa = 4.72GSANPAIFGGNDD245 pKa = 2.68NSYY248 pKa = 11.11NAGTMKK254 pKa = 10.33YY255 pKa = 9.56FQVRR259 pKa = 11.84YY260 pKa = 10.06SGFVLGANSEE270 pKa = 4.36LQSLTAEE277 pKa = 4.96GIGDD281 pKa = 4.12GTTLEE286 pKa = 4.48YY287 pKa = 10.22FQSVNSSDD295 pKa = 4.05DD296 pKa = 3.38GAEE299 pKa = 3.95FFGGKK304 pKa = 9.24VKK306 pKa = 10.57FKK308 pKa = 10.83HH309 pKa = 5.87YY310 pKa = 10.16IAVNADD316 pKa = 3.82DD317 pKa = 6.11DD318 pKa = 6.05SIDD321 pKa = 4.56LDD323 pKa = 4.04TGLEE327 pKa = 4.56GYY329 pKa = 9.16FQYY332 pKa = 11.62LLLLQRR338 pKa = 11.84DD339 pKa = 3.91GDD341 pKa = 3.95GDD343 pKa = 4.13ALMEE347 pKa = 4.69IDD349 pKa = 4.46SNGAEE354 pKa = 4.25SDD356 pKa = 3.6TPRR359 pKa = 11.84QKK361 pKa = 9.84TILANFTGVQPSDD374 pKa = 3.31NANSDD379 pKa = 3.56DD380 pKa = 3.8AAVLIRR386 pKa = 11.84GNSDD390 pKa = 2.9ITWANGIINVPNNEE404 pKa = 4.07CLRR407 pKa = 11.84LNGSGTVPATITADD421 pKa = 3.7SVVMTCNSAIYY432 pKa = 9.96LGTGTYY438 pKa = 8.26TAADD442 pKa = 3.7TEE444 pKa = 4.5AQFTAGTNNDD454 pKa = 3.49DD455 pKa = 4.09TFTSTLTSTFVNGSNEE471 pKa = 4.2SGVPAFDD478 pKa = 5.53ANTLDD483 pKa = 4.33SFFDD487 pKa = 3.4TTDD490 pKa = 3.33YY491 pKa = 11.11IGAVKK496 pKa = 10.35DD497 pKa = 5.16AADD500 pKa = 2.82TWYY503 pKa = 10.85RR504 pKa = 11.84GWTCDD509 pKa = 4.03NATADD514 pKa = 4.05FGSGSACTALPTTT527 pKa = 4.63

Molecular weight: 54.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B1ZVH2|A0A0B1ZVH2_9SPHN Uncharacterized protein OS=Novosphingobium malaysiense OX=1348853 GN=LK12_02185 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.54GFRR19 pKa = 11.84ARR21 pKa = 11.84TATAGGRR28 pKa = 11.84KK29 pKa = 8.05VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4270

0

4270

1374240

36

2676

321.8

34.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.327 ± 0.058

0.947 ± 0.012

6.067 ± 0.029

6.071 ± 0.031

3.654 ± 0.021

8.834 ± 0.032

2.103 ± 0.017

4.883 ± 0.028

3.291 ± 0.031

9.532 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.664 ± 0.02

2.662 ± 0.024

5.289 ± 0.027

3.101 ± 0.02

6.841 ± 0.036

5.429 ± 0.028

5.231 ± 0.029

7.219 ± 0.028

1.467 ± 0.014

2.386 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski