Bacillus phage phi4J1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rockvillevirus; Bacillus virus phi4J1

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2MVF0|A0A0S2MVF0_9CAUD Phage_Mu_F domain-containing protein OS=Bacillus phage phi4J1 OX=1643326 GN=XO28_0004 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.12VFKK5 pKa = 10.53MNDD8 pKa = 3.27IDD10 pKa = 3.98WVCAEE15 pKa = 4.44TEE17 pKa = 4.22EE18 pKa = 4.84QAKK21 pKa = 10.01EE22 pKa = 4.04YY23 pKa = 10.75YY24 pKa = 9.95KK25 pKa = 10.38EE26 pKa = 3.86EE27 pKa = 4.48CGIGDD32 pKa = 4.0EE33 pKa = 4.91DD34 pKa = 4.22LNEE37 pKa = 4.04YY38 pKa = 10.85FEE40 pKa = 5.7GEE42 pKa = 4.16VSLQEE47 pKa = 4.08TMHH50 pKa = 6.99INVDD54 pKa = 3.6DD55 pKa = 4.4LPYY58 pKa = 10.83EE59 pKa = 4.24EE60 pKa = 5.08QQQCQTMMHH69 pKa = 6.8RR70 pKa = 11.84GGEE73 pKa = 4.25LVVLRR78 pKa = 11.84SFEE81 pKa = 4.01WAIKK85 pKa = 9.54QNNITKK91 pKa = 8.96PCVIASTEE99 pKa = 4.1YY100 pKa = 11.01

Molecular weight: 11.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2MVE5|A0A0S2MVE5_9CAUD Uncharacterized protein OS=Bacillus phage phi4J1 OX=1643326 GN=XO28_0058 PE=4 SV=1
MM1 pKa = 7.69SMKK4 pKa = 10.14FQSNRR9 pKa = 11.84AAVMARR15 pKa = 11.84HH16 pKa = 6.01LAAKK20 pKa = 9.57KK21 pKa = 9.61AAHH24 pKa = 5.72TAVGQFVSSKK34 pKa = 10.78AKK36 pKa = 10.47LLAAVDD42 pKa = 3.87TGNLRR47 pKa = 11.84SSISSKK53 pKa = 11.32AEE55 pKa = 3.67LEE57 pKa = 4.12KK58 pKa = 11.22VVIGTSADD66 pKa = 3.19YY67 pKa = 10.84GIYY70 pKa = 9.53VEE72 pKa = 5.28KK73 pKa = 10.12GTGVYY78 pKa = 10.35AVDD81 pKa = 3.37GDD83 pKa = 4.07GRR85 pKa = 11.84KK86 pKa = 7.31TPWMYY91 pKa = 11.02RR92 pKa = 11.84DD93 pKa = 3.52PKK95 pKa = 8.09TGKK98 pKa = 8.46MVKK101 pKa = 8.29TQGQHH106 pKa = 4.99AQPFLRR112 pKa = 11.84PAAEE116 pKa = 4.21NNKK119 pKa = 9.76PMITQAATRR128 pKa = 11.84TYY130 pKa = 10.64SSLMRR135 pKa = 5.16

Molecular weight: 14.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

12805

38

1618

191.1

21.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.333 ± 0.502

0.867 ± 0.134

5.537 ± 0.24

8.38 ± 0.388

4.092 ± 0.2

6.341 ± 0.362

1.578 ± 0.137

7.325 ± 0.215

9.02 ± 0.294

7.763 ± 0.425

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.936 ± 0.229

5.529 ± 0.226

3.124 ± 0.173

4.116 ± 0.212

4.498 ± 0.235

5.381 ± 0.175

5.56 ± 0.397

6.615 ± 0.288

1.421 ± 0.144

3.585 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski