Jiangella rhizosphaerae
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6027 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A418KPU2|A0A418KPU2_9ACTN Iron ABC transporter permease OS=Jiangella rhizosphaerae OX=2293569 GN=DY240_14775 PE=3 SV=1
MM1 pKa = 7.43 PVSPRR6 pKa = 11.84 RR7 pKa = 11.84 SVVLGTVAIALALGACGGGDD27 pKa = 4.18 DD28 pKa = 4.32 GGEE31 pKa = 3.88 AAGEE35 pKa = 4.1 GDD37 pKa = 3.29 NVVTVRR43 pKa = 11.84 GCNPQSAFVPANSNEE58 pKa = 4.1 VCSGDD63 pKa = 3.73 ILDD66 pKa = 3.97 QVFSFLIRR74 pKa = 11.84 YY75 pKa = 8.34 DD76 pKa = 3.7 AEE78 pKa = 4.22 TGEE81 pKa = 4.33 PTNEE85 pKa = 3.36 IAADD89 pKa = 3.12 ITTPDD94 pKa = 4.02 NGLTWTITLEE104 pKa = 4.66 DD105 pKa = 3.48 GWTFHH110 pKa = 7.46 DD111 pKa = 5.33 GSPVTAQSFVDD122 pKa = 3.58 AWNWAAYY129 pKa = 9.73 GDD131 pKa = 3.99 NATLNSYY138 pKa = 10.16 FFEE141 pKa = 5.46 PIQGYY146 pKa = 10.57 AEE148 pKa = 4.1 VQNQVDD154 pKa = 3.59 ADD156 pKa = 3.96 GNPIEE161 pKa = 4.71 GTAQAEE167 pKa = 4.51 TMSGLEE173 pKa = 4.09 VVDD176 pKa = 5.14 DD177 pKa = 3.7 LTFNVTLTQAEE188 pKa = 4.59 SQFPMRR194 pKa = 11.84 LGYY197 pKa = 8.26 TAFAPLPEE205 pKa = 4.56 SFYY208 pKa = 10.99 EE209 pKa = 4.22 DD210 pKa = 3.64 PEE212 pKa = 4.91 AFGQHH217 pKa = 7.0 PIGTGPFQFEE227 pKa = 3.93 SWEE230 pKa = 4.01 PNIDD234 pKa = 3.49 VRR236 pKa = 11.84 LTAYY240 pKa = 9.64 PDD242 pKa = 3.42 YY243 pKa = 11.2 NGDD246 pKa = 3.61 VKK248 pKa = 10.98 PSIDD252 pKa = 3.16 GAIYY256 pKa = 10.2 RR257 pKa = 11.84 IYY259 pKa = 10.84 EE260 pKa = 4.2 NDD262 pKa = 3.31 DD263 pKa = 2.99 AAYY266 pKa = 10.95 NDD268 pKa = 4.05 LQADD272 pKa = 3.79 QLDD275 pKa = 4.16 IMPLLPTSALAGDD288 pKa = 4.72 TYY290 pKa = 10.94 RR291 pKa = 11.84 IDD293 pKa = 3.51 LGDD296 pKa = 3.47 RR297 pKa = 11.84 FISRR301 pKa = 11.84 EE302 pKa = 3.82 TGVFEE307 pKa = 5.15 TITFAPASVDD317 pKa = 3.23 EE318 pKa = 4.8 RR319 pKa = 11.84 FADD322 pKa = 3.55 PRR324 pKa = 11.84 IRR326 pKa = 11.84 QAISMAINRR335 pKa = 11.84 EE336 pKa = 4.09 EE337 pKa = 3.75 IVEE340 pKa = 4.21 QIFSGARR347 pKa = 11.84 TPATGWVSPVVVNGYY362 pKa = 9.52 VEE364 pKa = 4.66 GACGEE369 pKa = 4.29 FCTYY373 pKa = 11.12 DD374 pKa = 3.24 PGAAQDD380 pKa = 5.34 LYY382 pKa = 11.21 AQTDD386 pKa = 4.79 GIDD389 pKa = 3.6 GPFPLSYY396 pKa = 10.81 NADD399 pKa = 3.69 SDD401 pKa = 3.69 HH402 pKa = 6.55 KK403 pKa = 10.19 AWVEE407 pKa = 3.94 AVCNQIDD414 pKa = 3.44 ATLPVDD420 pKa = 4.17 CVPTPFVDD428 pKa = 4.01 LATFRR433 pKa = 11.84 NEE435 pKa = 3.17 ITNRR439 pKa = 11.84 EE440 pKa = 4.11 LTGMFRR446 pKa = 11.84 TGWQMDD452 pKa = 4.0 YY453 pKa = 11.08 PSLEE457 pKa = 4.05 NFLVPIFATGASANDD472 pKa = 3.52 GDD474 pKa = 4.16 YY475 pKa = 11.66 SNAEE479 pKa = 3.67 FDD481 pKa = 3.93 QLVEE485 pKa = 3.99 EE486 pKa = 4.56 AARR489 pKa = 11.84 TEE491 pKa = 4.37 GEE493 pKa = 4.26 AGAEE497 pKa = 4.27 LYY499 pKa = 10.47 RR500 pKa = 11.84 QAEE503 pKa = 3.97 ALLRR507 pKa = 11.84 NDD509 pKa = 3.73 MPAIPLWHH517 pKa = 6.76 RR518 pKa = 11.84 VNIAGYY524 pKa = 8.69 SSHH527 pKa = 7.17 VEE529 pKa = 3.94 NVQLTVFQTVDD540 pKa = 3.73 LLSVTTTGG548 pKa = 3.78
Molecular weight: 59.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.592
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.923
Patrickios 1.163
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|A0A418KPX7|A0A418KPX7_9ACTN Type II toxin-antitoxin system Phd/YefM family antitoxin OS=Jiangella rhizosphaerae OX=2293569 GN=DY240_14385 PE=3 SV=1
MM1 pKa = 7.41 VKK3 pKa = 9.03 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 10.78 GRR40 pKa = 11.84 AKK42 pKa = 10.73 LSAA45 pKa = 3.92
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6027
0
6027
1902621
29
3103
315.7
33.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.932 ± 0.049
0.622 ± 0.008
6.685 ± 0.027
5.61 ± 0.033
2.787 ± 0.019
9.42 ± 0.03
2.103 ± 0.015
3.313 ± 0.023
1.363 ± 0.018
10.23 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.646 ± 0.013
1.686 ± 0.017
5.997 ± 0.027
2.547 ± 0.015
8.017 ± 0.038
4.847 ± 0.022
6.027 ± 0.028
9.477 ± 0.03
1.603 ± 0.016
2.087 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here