Streptomyces sp. BK308

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8312 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R3D9T0|A0A4R3D9T0_9ACTN RNA polymerase ECF family sigma subunit OS=Streptomyces sp. BK308 OX=2512170 GN=EV587_103468 PE=4 SV=1
MM1 pKa = 7.31SACALLGCLTSLTACGDD18 pKa = 3.67GGDD21 pKa = 4.11GGGPGASTDD30 pKa = 3.03AGAPVTAVSTAATGPGTATAAPAGTPSEE58 pKa = 4.57EE59 pKa = 4.15EE60 pKa = 3.89TTAPEE65 pKa = 4.31PATSSDD71 pKa = 3.54DD72 pKa = 3.49TATAGTDD79 pKa = 3.11AVGAQTPSGVPRR91 pKa = 11.84GTWTGTAHH99 pKa = 6.23VSVDD103 pKa = 4.04FADD106 pKa = 4.59PGCATASRR114 pKa = 11.84SYY116 pKa = 10.98DD117 pKa = 3.41LPATLVIEE125 pKa = 4.66DD126 pKa = 4.16PVGSEE131 pKa = 4.12SNSFHH136 pKa = 6.92LSWASDD142 pKa = 3.44SQTTEE147 pKa = 3.6GAFGVTSALSGTDD160 pKa = 3.17TTDD163 pKa = 2.85GRR165 pKa = 11.84GVEE168 pKa = 3.67IAYY171 pKa = 9.02WSLSDD176 pKa = 5.6DD177 pKa = 3.93GGGDD181 pKa = 3.37LSGRR185 pKa = 11.84LTDD188 pKa = 3.47SAAAEE193 pKa = 4.21GASLNLLFVPKK204 pKa = 10.11PLFQCSSYY212 pKa = 11.02TNYY215 pKa = 10.03PYY217 pKa = 11.5GMAVGTSLTGTVTGGSASLTLSGTSADD244 pKa = 3.51GTRR247 pKa = 11.84SFRR250 pKa = 11.84VAWSS254 pKa = 3.24

Molecular weight: 24.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R3CME9|A0A4R3CME9_9ACTN Magnesium chelatase family protein OS=Streptomyces sp. BK308 OX=2512170 GN=EV587_11380 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.72HH17 pKa = 5.74RR18 pKa = 11.84KK19 pKa = 7.77LLKK22 pKa = 8.07RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8312

0

8312

2717867

25

5419

327.0

35.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.606 ± 0.047

0.811 ± 0.007

5.953 ± 0.023

5.581 ± 0.027

2.702 ± 0.017

9.483 ± 0.026

2.395 ± 0.012

2.978 ± 0.02

2.115 ± 0.022

10.405 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.689 ± 0.011

1.743 ± 0.014

6.123 ± 0.027

2.772 ± 0.016

8.196 ± 0.029

5.007 ± 0.023

6.224 ± 0.025

8.532 ± 0.022

1.556 ± 0.012

2.129 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski