Alstroemeria necrotic streak virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Tospoviridae; Orthotospovirus; Alstroemeria necrotic streak orthotospovirus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3B8DZR5|A0A3B8DZR5_9VIRU Replicase OS=Alstroemeria necrotic streak virus OX=693450 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.38LIKK5 pKa = 10.41LLEE8 pKa = 4.2LMVKK12 pKa = 10.55VSLFTIALSSILLSFLIFKK31 pKa = 9.23ATDD34 pKa = 3.11AKK36 pKa = 11.27VEE38 pKa = 4.54IIRR41 pKa = 11.84GDD43 pKa = 3.37HH44 pKa = 6.67PEE46 pKa = 4.52FYY48 pKa = 10.55DD49 pKa = 4.39DD50 pKa = 4.24SYY52 pKa = 12.03EE53 pKa = 4.18NEE55 pKa = 4.57LPVALPNQQKK65 pKa = 7.82TALEE69 pKa = 4.25TSTNLMLDD77 pKa = 3.37ASSIRR82 pKa = 11.84SRR84 pKa = 11.84QEE86 pKa = 3.24RR87 pKa = 11.84DD88 pKa = 3.06GKK90 pKa = 10.74LATASKK96 pKa = 10.5SDD98 pKa = 3.39SVTQKK103 pKa = 9.75TISVLDD109 pKa = 3.98LPNNCLNASLLKK121 pKa = 10.85CEE123 pKa = 4.29IKK125 pKa = 10.71GVSTYY130 pKa = 10.45NVYY133 pKa = 10.71YY134 pKa = 10.13QVEE137 pKa = 4.33NDD139 pKa = 3.66GVIYY143 pKa = 10.72SCVSDD148 pKa = 3.51TTEE151 pKa = 4.24SLGRR155 pKa = 11.84CDD157 pKa = 4.85NSQNLPKK164 pKa = 10.24KK165 pKa = 9.48FPEE168 pKa = 4.19VPVIPITRR176 pKa = 11.84LDD178 pKa = 3.75NKK180 pKa = 10.14RR181 pKa = 11.84HH182 pKa = 5.45FSVGTKK188 pKa = 9.6FFISEE193 pKa = 4.13SLAHH197 pKa = 6.34GNYY200 pKa = 9.39PITYY204 pKa = 9.75NSYY207 pKa = 8.05PTNGTVSLQTVKK219 pKa = 10.92LSGDD223 pKa = 3.73CKK225 pKa = 8.95ITKK228 pKa = 10.41SNFANPYY235 pKa = 7.71TVSITSPEE243 pKa = 4.14KK244 pKa = 10.56IMGYY248 pKa = 9.72LIKK251 pKa = 10.74KK252 pKa = 8.59PGEE255 pKa = 4.17DD256 pKa = 3.89AEE258 pKa = 4.6HH259 pKa = 6.44KK260 pKa = 10.38VIPFSGSTSITFSEE274 pKa = 4.16EE275 pKa = 3.91MLDD278 pKa = 3.68GDD280 pKa = 5.32HH281 pKa = 7.3NLLCGDD287 pKa = 4.55KK288 pKa = 10.39SAKK291 pKa = 9.51IPKK294 pKa = 7.78TNKK297 pKa = 9.47RR298 pKa = 11.84VRR300 pKa = 11.84DD301 pKa = 4.09CIIKK305 pKa = 9.92YY306 pKa = 10.28SKK308 pKa = 10.42SIYY311 pKa = 9.82KK312 pKa = 8.12QTACINFSWIRR323 pKa = 11.84LILIALLIYY332 pKa = 9.71FPIRR336 pKa = 11.84WLVNKK341 pKa = 6.89TTKK344 pKa = 10.24PLFLWYY350 pKa = 10.08DD351 pKa = 4.09LIGLITYY358 pKa = 7.77PILLILNYY366 pKa = 9.22LWKK369 pKa = 10.56YY370 pKa = 10.82FPFKK374 pKa = 10.67CSNCGNLCIVTHH386 pKa = 6.74EE387 pKa = 4.21CTKK390 pKa = 10.74RR391 pKa = 11.84CICNKK396 pKa = 9.61SKK398 pKa = 10.94ASRR401 pKa = 11.84EE402 pKa = 4.06HH403 pKa = 7.99SGEE406 pKa = 4.04CPILSKK412 pKa = 10.86EE413 pKa = 4.06ADD415 pKa = 3.41HH416 pKa = 7.0EE417 pKa = 4.6YY418 pKa = 10.82KK419 pKa = 9.81KK420 pKa = 10.43HH421 pKa = 5.83KK422 pKa = 6.45WTSMEE427 pKa = 3.86WFHH430 pKa = 7.65LIVNTKK436 pKa = 10.62LSLGLLKK443 pKa = 10.69FITEE447 pKa = 4.19LLIGLVILSQMPMSMAQTTQCLNGCFYY474 pKa = 11.52VPGCSYY480 pKa = 11.23LVTSKK485 pKa = 10.98FEE487 pKa = 3.99KK488 pKa = 10.67CPEE491 pKa = 4.05KK492 pKa = 10.71DD493 pKa = 2.88QCYY496 pKa = 10.9CNVQDD501 pKa = 4.94DD502 pKa = 4.99KK503 pKa = 11.2IIEE506 pKa = 4.67SIFGTNIVIEE516 pKa = 4.56GPNDD520 pKa = 3.95CIANQICSASSSIDD534 pKa = 3.41SLVKK538 pKa = 10.4CRR540 pKa = 11.84LGCEE544 pKa = 3.91YY545 pKa = 11.36LDD547 pKa = 4.03LFKK550 pKa = 11.16SKK552 pKa = 10.12PLYY555 pKa = 10.48KK556 pKa = 10.54GFPDD560 pKa = 3.47YY561 pKa = 10.88TGNSLGLTSVGLYY574 pKa = 5.74VTKK577 pKa = 10.65RR578 pKa = 11.84LRR580 pKa = 11.84NGIIDD585 pKa = 4.06SYY587 pKa = 11.43NGTDD591 pKa = 3.46RR592 pKa = 11.84ISGIVAGDD600 pKa = 3.71SLDD603 pKa = 4.57KK604 pKa = 11.24NDD606 pKa = 3.81TNIPEE611 pKa = 4.68NILPRR616 pKa = 11.84QSLIFDD622 pKa = 3.57STVDD626 pKa = 3.15GKK628 pKa = 10.53YY629 pKa = 10.28RR630 pKa = 11.84YY631 pKa = 9.28MIEE634 pKa = 3.85QALLGGGGTVFMLNDD649 pKa = 3.78KK650 pKa = 10.43TSGTAKK656 pKa = 10.58KK657 pKa = 8.37FTIYY661 pKa = 9.85IKK663 pKa = 10.66SVGVHH668 pKa = 5.02YY669 pKa = 10.32EE670 pKa = 3.8VAEE673 pKa = 4.53KK674 pKa = 9.97YY675 pKa = 5.9TTAPIQSTHH684 pKa = 5.09TDD686 pKa = 4.13FYY688 pKa = 10.5STCTGNCDD696 pKa = 3.29TCRR699 pKa = 11.84KK700 pKa = 8.9NQALTGFQDD709 pKa = 3.87FCITPTSYY717 pKa = 9.64WGCEE721 pKa = 3.99EE722 pKa = 4.13AWCFAINEE730 pKa = 4.33GATCGFCRR738 pKa = 11.84NVYY741 pKa = 11.08DD742 pKa = 3.24MDD744 pKa = 3.57KK745 pKa = 10.63SYY747 pKa = 11.12RR748 pKa = 11.84IYY750 pKa = 11.05SVLKK754 pKa = 7.88STIVADD760 pKa = 3.79VCVSGILGSHH770 pKa = 6.01CTRR773 pKa = 11.84ITEE776 pKa = 4.08EE777 pKa = 3.99VPYY780 pKa = 10.66EE781 pKa = 3.96NTIFQADD788 pKa = 3.53IQADD792 pKa = 3.72LHH794 pKa = 6.74NDD796 pKa = 4.41GITIGEE802 pKa = 4.79LIAHH806 pKa = 6.91GPDD809 pKa = 2.87SHH811 pKa = 8.27IYY813 pKa = 9.65SGNIANMNDD822 pKa = 3.43PVKK825 pKa = 10.48MFGHH829 pKa = 6.71PQLTHH834 pKa = 6.86DD835 pKa = 4.72GVPIFTKK842 pKa = 9.46KK843 pKa = 8.72TLEE846 pKa = 4.3GEE848 pKa = 4.15DD849 pKa = 4.8LSWDD853 pKa = 3.51CAAIGKK859 pKa = 10.08KK860 pKa = 10.03SVTIKK865 pKa = 9.08TCGYY869 pKa = 7.5DD870 pKa = 3.12TYY872 pKa = 10.89RR873 pKa = 11.84FKK875 pKa = 11.22SGLEE879 pKa = 3.96QISDD883 pKa = 3.4IPVIFKK889 pKa = 10.6DD890 pKa = 4.57FSSFFLEE897 pKa = 4.62KK898 pKa = 10.68SFNLGKK904 pKa = 10.72LKK906 pKa = 10.27MVVDD910 pKa = 5.14LPLDD914 pKa = 3.8LFKK917 pKa = 11.04VAPKK921 pKa = 10.12KK922 pKa = 10.48PSITSTRR929 pKa = 11.84LEE931 pKa = 3.94CSGCLLCGQGLSCNMEE947 pKa = 4.13FFSDD951 pKa = 3.92LTFSTAISIDD961 pKa = 3.6VCSLSTYY968 pKa = 10.46QLAIKK973 pKa = 10.13KK974 pKa = 10.17GSNKK978 pKa = 10.01YY979 pKa = 10.25NITMYY984 pKa = 10.77CSVNPDD990 pKa = 3.1KK991 pKa = 11.19KK992 pKa = 11.29KK993 pKa = 9.79MVLYY997 pKa = 10.32PEE999 pKa = 4.97GNPDD1003 pKa = 3.43LSVEE1007 pKa = 4.08VLINNVVVEE1016 pKa = 4.41EE1017 pKa = 4.55PEE1019 pKa = 4.55NIIDD1023 pKa = 4.1QNDD1026 pKa = 3.55EE1027 pKa = 4.09YY1028 pKa = 11.62AHH1030 pKa = 7.07EE1031 pKa = 4.17EE1032 pKa = 3.71QQYY1035 pKa = 11.29NSDD1038 pKa = 3.33SSAWSFWDD1046 pKa = 4.02YY1047 pKa = 11.1IKK1049 pKa = 11.3SPFNFIASYY1058 pKa = 10.19FGSFFDD1064 pKa = 4.2TIRR1067 pKa = 11.84VILLIAFIFLVVYY1080 pKa = 8.42FCSMLTSMCKK1090 pKa = 10.33GYY1092 pKa = 10.38IKK1094 pKa = 10.75NEE1096 pKa = 4.15SYY1098 pKa = 11.03KK1099 pKa = 10.98SRR1101 pKa = 11.84DD1102 pKa = 3.47KK1103 pKa = 11.84VEE1105 pKa = 5.44DD1106 pKa = 4.48DD1107 pKa = 5.11DD1108 pKa = 6.78DD1109 pKa = 6.19SEE1111 pKa = 4.65TTKK1114 pKa = 10.82PLLIKK1119 pKa = 10.22DD1120 pKa = 3.47TMTRR1124 pKa = 11.84RR1125 pKa = 11.84RR1126 pKa = 11.84PPMDD1130 pKa = 3.92FSHH1133 pKa = 6.98LVV1135 pKa = 3.07

Molecular weight: 127.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3B8DZQ8|A0A3B8DZQ8_9VIRU Envelopment polyprotein OS=Alstroemeria necrotic streak virus OX=693450 PE=3 SV=1
MM1 pKa = 7.54SKK3 pKa = 11.03AKK5 pKa = 8.99LTRR8 pKa = 11.84EE9 pKa = 3.98NIINLLTQSAEE20 pKa = 4.05VEE22 pKa = 4.25FEE24 pKa = 4.08EE25 pKa = 4.92EE26 pKa = 3.79QNQAAFNFQNFCQDD40 pKa = 3.11NLDD43 pKa = 3.96LVKK46 pKa = 10.75KK47 pKa = 9.99MSITSCLTFLKK58 pKa = 10.42NRR60 pKa = 11.84QSIMKK65 pKa = 9.42VIKK68 pKa = 10.61QSDD71 pKa = 3.74FTFGRR76 pKa = 11.84ITIKK80 pKa = 9.67KK81 pKa = 7.02TSEE84 pKa = 4.1RR85 pKa = 11.84IGATDD90 pKa = 3.11MTFRR94 pKa = 11.84RR95 pKa = 11.84LDD97 pKa = 3.09SMIRR101 pKa = 11.84VKK103 pKa = 10.81LIQEE107 pKa = 3.89TANAVNLDD115 pKa = 3.94AIKK118 pKa = 9.93TKK120 pKa = 10.22IASHH124 pKa = 6.91PLVQAYY130 pKa = 8.31GLPLNDD136 pKa = 3.59AKK138 pKa = 10.48SVRR141 pKa = 11.84LAIMLGGSIPLIASVDD157 pKa = 3.85SFEE160 pKa = 4.77MISVVLAIYY169 pKa = 10.44QDD171 pKa = 4.14AKK173 pKa = 11.24CKK175 pKa = 10.5DD176 pKa = 3.35LGINPKK182 pKa = 10.55KK183 pKa = 10.72YY184 pKa = 8.92DD185 pKa = 3.46TKK187 pKa = 10.19EE188 pKa = 3.85ALGKK192 pKa = 10.3VCTVLKK198 pKa = 10.79SKK200 pKa = 10.33GFSMDD205 pKa = 3.23DD206 pKa = 3.39TQVTKK211 pKa = 10.6GRR213 pKa = 11.84EE214 pKa = 3.67YY215 pKa = 11.16AAILSSCNPNAKK227 pKa = 9.97GSVAMEE233 pKa = 4.51HH234 pKa = 6.33YY235 pKa = 10.41SEE237 pKa = 4.88HH238 pKa = 6.42LNKK241 pKa = 9.79FYY243 pKa = 11.24EE244 pKa = 4.11MFGVKK249 pKa = 9.69KK250 pKa = 9.53EE251 pKa = 4.2SKK253 pKa = 10.02ISGVAA258 pKa = 2.97

Molecular weight: 28.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

5046

258

2874

1009.2

114.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.162 ± 0.518

2.279 ± 0.615

5.906 ± 0.297

6.817 ± 0.865

4.38 ± 0.254

4.261 ± 0.501

1.744 ± 0.186

8.125 ± 0.206

8.66 ± 0.356

9.81 ± 0.495

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.874 ± 0.381

6.223 ± 0.372

3.032 ± 0.57

2.893 ± 0.48

3.428 ± 0.379

9.176 ± 0.762

6.044 ± 0.333

5.371 ± 0.42

0.832 ± 0.188

3.904 ± 0.389
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski