Volvox carteri f. nagariensis

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae; CS clade; Chlamydomonadales; Volvocaceae; Volvox; Volvox carteri

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14335 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8TXQ2|D8TXQ2_VOLCA Uncharacterized protein OS=Volvox carteri f. nagariensis OX=3068 GN=VOLCADRAFT_105002 PE=4 SV=1
MM1 pKa = 7.53GKK3 pKa = 9.82YY4 pKa = 9.21AQLVIGPAGCGKK16 pKa = 8.65STYY19 pKa = 10.06CNHH22 pKa = 7.24LYY24 pKa = 9.39EE25 pKa = 4.86HH26 pKa = 6.75CQAIKK31 pKa = 10.53RR32 pKa = 11.84SVHH35 pKa = 5.37VVNLDD40 pKa = 3.43PAAEE44 pKa = 4.1AFQYY48 pKa = 10.15PVSLDD53 pKa = 3.26IRR55 pKa = 11.84DD56 pKa = 4.07LVCLEE61 pKa = 4.6DD62 pKa = 3.82VMEE65 pKa = 4.44EE66 pKa = 4.52LGLGPNGGLLYY77 pKa = 10.84CMEE80 pKa = 4.19YY81 pKa = 11.05LEE83 pKa = 5.95DD84 pKa = 5.57NLHH87 pKa = 5.78EE88 pKa = 4.25WLGEE92 pKa = 3.93EE93 pKa = 4.37LEE95 pKa = 4.78GYY97 pKa = 10.47GDD99 pKa = 3.54EE100 pKa = 5.19DD101 pKa = 4.61YY102 pKa = 11.56LVFDD106 pKa = 4.74CPGQIEE112 pKa = 5.27LYY114 pKa = 9.65NHH116 pKa = 6.79LSVFRR121 pKa = 11.84SFVDD125 pKa = 3.84FLKK128 pKa = 10.89NDD130 pKa = 3.12GWSVCVVYY138 pKa = 10.83CLDD141 pKa = 3.18AHH143 pKa = 6.55FVTDD147 pKa = 3.28VAKK150 pKa = 10.73FMAGALQALAAMVKK164 pKa = 10.63LEE166 pKa = 4.29LPHH169 pKa = 8.09VNILTKK175 pKa = 10.44VDD177 pKa = 3.4LMEE180 pKa = 5.67DD181 pKa = 3.63KK182 pKa = 11.25NHH184 pKa = 6.76LDD186 pKa = 3.33EE187 pKa = 6.13FLFPDD192 pKa = 4.48PEE194 pKa = 4.76LLLHH198 pKa = 6.19QLAASTGPRR207 pKa = 11.84FRR209 pKa = 11.84QLNRR213 pKa = 11.84AMGGLLEE220 pKa = 4.39EE221 pKa = 4.77FSLVSFLPLDD231 pKa = 3.51ITDD234 pKa = 4.13EE235 pKa = 4.28DD236 pKa = 4.62SIADD240 pKa = 3.44ILGQIDD246 pKa = 3.42MAIQYY251 pKa = 10.78GEE253 pKa = 4.07DD254 pKa = 3.36AEE256 pKa = 4.18PRR258 pKa = 11.84IRR260 pKa = 11.84DD261 pKa = 3.65EE262 pKa = 5.56LEE264 pKa = 4.61DD265 pKa = 5.27GGDD268 pKa = 4.71DD269 pKa = 5.47DD270 pKa = 6.97DD271 pKa = 7.51DD272 pKa = 6.64DD273 pKa = 6.4GGGDD277 pKa = 3.7GGDD280 pKa = 4.53DD281 pKa = 3.09II282 pKa = 6.89

Molecular weight: 31.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D8TS10|D8TS10_VOLCA Uncharacterized protein (Fragment) OS=Volvox carteri f. nagariensis OX=3068 GN=VOLCADRAFT_59125 PE=4 SV=1
PP1 pKa = 6.77HH2 pKa = 6.72TRR4 pKa = 11.84GPHH7 pKa = 4.23TRR9 pKa = 11.84GPHH12 pKa = 4.27TRR14 pKa = 11.84GPHH17 pKa = 4.27TRR19 pKa = 11.84GPHH22 pKa = 4.27TRR24 pKa = 11.84GPHH27 pKa = 4.27TRR29 pKa = 11.84GPHH32 pKa = 4.21TRR34 pKa = 11.84GAHH37 pKa = 4.37TRR39 pKa = 11.84GPHH42 pKa = 4.48TRR44 pKa = 11.84GAHH47 pKa = 4.37TRR49 pKa = 11.84GPHH52 pKa = 4.48TRR54 pKa = 11.84GPHH57 pKa = 4.27TRR59 pKa = 11.84GPHH62 pKa = 4.27TRR64 pKa = 11.84GPHH67 pKa = 4.27TRR69 pKa = 11.84GPHH72 pKa = 4.49TRR74 pKa = 11.84GPHH77 pKa = 4.71

Molecular weight: 8.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14333

2

14335

8189894

49

22244

571.3

60.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.297 ± 0.052

1.65 ± 0.012

4.715 ± 0.013

5.211 ± 0.026

2.493 ± 0.015

9.546 ± 0.035

2.26 ± 0.009

2.781 ± 0.015

3.026 ± 0.019

9.227 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.075 ± 0.009

2.497 ± 0.014

7.005 ± 0.032

4.462 ± 0.021

6.674 ± 0.018

7.645 ± 0.025

5.307 ± 0.012

6.794 ± 0.019

1.232 ± 0.007

2.103 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski