Uncinocarpus reesii (strain UAMH 1704)
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7760 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C4JMP0|C4JMP0_UNCRE DNA-directed RNA polymerase subunit beta OS=Uncinocarpus reesii (strain UAMH 1704) OX=336963 GN=UREG_04098 PE=3 SV=1
MM1 pKa = 7.64 EE2 pKa = 5.16 KK3 pKa = 10.27 CCNSPVVTYY12 pKa = 11.1 GEE14 pKa = 4.27 DD15 pKa = 3.1 CGMYY19 pKa = 10.2 CLASGGTVGEE29 pKa = 4.25 LVRR32 pKa = 11.84 CIIGDD37 pKa = 4.13 GVADD41 pKa = 3.93 GQVFCNMASNATATGSATPTATNSDD66 pKa = 4.0 RR67 pKa = 11.84 DD68 pKa = 4.62 DD69 pKa = 5.51 DD70 pKa = 6.21 DD71 pKa = 6.14 DD72 pKa = 4.74 DD73 pKa = 4.58 KK74 pKa = 11.82 KK75 pKa = 10.72 PTGTEE80 pKa = 3.74 TSPAATSSNAAFASVPQQPISKK102 pKa = 9.02 PAIGVLFTLFFSTFASVLFAA122 pKa = 5.45
Molecular weight: 12.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.961
IPC_protein 3.897
Toseland 3.681
ProMoST 4.088
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.872
Rodwell 3.732
Grimsley 3.592
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.279
Thurlkill 3.757
EMBOSS 3.872
Sillero 4.024
Patrickios 0.846
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.904
Protein with the highest isoelectric point:
>tr|C4JQG6|C4JQG6_UNCRE Uncharacterized protein OS=Uncinocarpus reesii (strain UAMH 1704) OX=336963 GN=UREG_04720 PE=4 SV=1
MM1 pKa = 8.09 DD2 pKa = 4.99 GGATRR7 pKa = 11.84 IDD9 pKa = 3.7 SEE11 pKa = 4.53 SFKK14 pKa = 10.94 ARR16 pKa = 11.84 RR17 pKa = 11.84 EE18 pKa = 3.85 FRR20 pKa = 11.84 EE21 pKa = 4.18 AALSLPPRR29 pKa = 11.84 EE30 pKa = 4.08 PHH32 pKa = 6.27 PWTPALRR39 pKa = 11.84 RR40 pKa = 11.84 HH41 pKa = 5.02 VWAALKK47 pKa = 10.83 GKK49 pKa = 9.74 GAVGILQPGQATTRR63 pKa = 11.84 SSQFRR68 pKa = 11.84 GSGPDD73 pKa = 3.18 AAPLLRR79 pKa = 11.84 TRR81 pKa = 11.84 LLDD84 pKa = 3.34 WSSDD88 pKa = 3.39 RR89 pKa = 11.84 DD90 pKa = 3.03 RR91 pKa = 11.84 DD92 pKa = 3.67 LFDD95 pKa = 4.49 RR96 pKa = 11.84 GGPRR100 pKa = 11.84 IQHH103 pKa = 5.98 RR104 pKa = 11.84 QTEE107 pKa = 4.33 LLDD110 pKa = 3.71 MGSSRR115 pKa = 11.84 LSRR118 pKa = 11.84 KK119 pKa = 9.66 GDD121 pKa = 3.15 EE122 pKa = 4.62 CGTVRR127 pKa = 11.84 LRR129 pKa = 11.84 GYY131 pKa = 11.29 KK132 pKa = 8.42 MLRR135 pKa = 11.84 GNGVCSSEE143 pKa = 3.66 MCAADD148 pKa = 3.87 RR149 pKa = 11.84 RR150 pKa = 11.84 AVLSYY155 pKa = 10.71 DD156 pKa = 3.39 YY157 pKa = 11.17 RR158 pKa = 5.68
Molecular weight: 17.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.399
IPC_protein 10.306
Toseland 10.496
ProMoST 10.511
Dawson 10.599
Bjellqvist 10.365
Wikipedia 10.847
Rodwell 10.657
Grimsley 10.657
Solomon 10.745
Lehninger 10.716
Nozaki 10.511
DTASelect 10.35
Thurlkill 10.511
EMBOSS 10.906
Sillero 10.555
Patrickios 10.394
IPC_peptide 10.76
IPC2_peptide 9.589
IPC2.peptide.svr19 8.725
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7760
0
7760
3724661
49
5757
480.0
53.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.212 ± 0.021
1.221 ± 0.011
5.618 ± 0.019
6.314 ± 0.027
3.723 ± 0.016
6.611 ± 0.024
2.416 ± 0.012
5.042 ± 0.019
5.082 ± 0.021
8.99 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.093 ± 0.01
3.777 ± 0.014
6.313 ± 0.032
4.049 ± 0.019
6.448 ± 0.023
8.473 ± 0.032
5.744 ± 0.017
5.875 ± 0.021
1.338 ± 0.009
2.66 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here