Devosia sp. Root436

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Devosiaceae; Devosia; unclassified Devosia

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3690 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7HW18|A0A0Q7HW18_9RHIZ GreA_GreB domain-containing protein OS=Devosia sp. Root436 OX=1736537 GN=ASD04_13455 PE=4 SV=1
MM1 pKa = 7.1THH3 pKa = 6.35FPCRR7 pKa = 11.84AAALAAGLMFSAGAMAQDD25 pKa = 3.63DD26 pKa = 4.56PGWHH30 pKa = 5.98GNAYY34 pKa = 10.65DD35 pKa = 3.91GMTTLFYY42 pKa = 10.77GVPQSDD48 pKa = 3.56HH49 pKa = 8.22VEE51 pKa = 3.9IALSCQAGSKK61 pKa = 9.66AANFVFAFAPIEE73 pKa = 4.19AVDD76 pKa = 4.18GVQVQVTLEE85 pKa = 4.2ADD87 pKa = 4.57DD88 pKa = 4.19ISLPIQTTGTLMQMDD103 pKa = 5.0DD104 pKa = 3.92LFLLEE109 pKa = 4.85GEE111 pKa = 4.63VAVDD115 pKa = 3.9ARR117 pKa = 11.84LIDD120 pKa = 4.13LLGSPGMLSVFVEE133 pKa = 4.9DD134 pKa = 5.06GAAEE138 pKa = 4.13YY139 pKa = 9.71PLEE142 pKa = 4.54GALDD146 pKa = 3.59AAAALIQACGQDD158 pKa = 3.61AEE160 pKa = 4.55TAAIEE165 pKa = 4.53SCDD168 pKa = 3.57LNAWIEE174 pKa = 4.26AEE176 pKa = 4.44GPAAQVIRR184 pKa = 11.84DD185 pKa = 4.05GPSANATAIADD196 pKa = 3.62MPQPYY201 pKa = 9.88EE202 pKa = 4.71GYY204 pKa = 10.77DD205 pKa = 3.24GMAYY209 pKa = 7.12PTASITGSRR218 pKa = 11.84DD219 pKa = 2.17GWFRR223 pKa = 11.84IKK225 pKa = 10.6EE226 pKa = 4.31VVTNLYY232 pKa = 10.95AEE234 pKa = 4.89ADD236 pKa = 3.78PVTVFSGEE244 pKa = 3.55GWVPGKK250 pKa = 10.59ALGLYY255 pKa = 9.67VEE257 pKa = 5.26SPALLDD263 pKa = 4.67RR264 pKa = 11.84PADD267 pKa = 4.0DD268 pKa = 4.13AAVAVDD274 pKa = 4.8FSDD277 pKa = 3.6GGEE280 pKa = 4.01NFLTVDD286 pKa = 3.67RR287 pKa = 11.84LYY289 pKa = 10.91DD290 pKa = 3.62CRR292 pKa = 11.84GDD294 pKa = 3.25WVEE297 pKa = 4.86IGGTYY302 pKa = 9.63AGKK305 pKa = 10.22RR306 pKa = 11.84VRR308 pKa = 11.84GWSSDD313 pKa = 2.82ICEE316 pKa = 4.32SQITTCSS323 pKa = 3.11

Molecular weight: 34.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7I9G1|A0A0Q7I9G1_9RHIZ NepR domain-containing protein OS=Devosia sp. Root436 OX=1736537 GN=ASD04_06510 PE=4 SV=1
MM1 pKa = 6.92TADD4 pKa = 3.44VTRR7 pKa = 11.84LIAYY11 pKa = 7.31YY12 pKa = 10.01KK13 pKa = 10.52SPLGRR18 pKa = 11.84ISRR21 pKa = 11.84ALVRR25 pKa = 11.84EE26 pKa = 3.98QVLGLAGDD34 pKa = 3.83VAGKK38 pKa = 10.37RR39 pKa = 11.84ILGLGFATPYY49 pKa = 9.98MRR51 pKa = 11.84FTLEE55 pKa = 3.34RR56 pKa = 11.84AEE58 pKa = 4.01RR59 pKa = 11.84VLAFMPARR67 pKa = 11.84QGASAWPRR75 pKa = 11.84EE76 pKa = 4.64GPSHH80 pKa = 6.71TVLCDD85 pKa = 3.6PLEE88 pKa = 4.41MPLTDD93 pKa = 3.66AAIDD97 pKa = 3.65LTVAIHH103 pKa = 6.15ALEE106 pKa = 4.48HH107 pKa = 5.66VADD110 pKa = 4.16AEE112 pKa = 4.11EE113 pKa = 4.41LMRR116 pKa = 11.84EE117 pKa = 4.55LWRR120 pKa = 11.84ITAPNGHH127 pKa = 7.34LLLVVPRR134 pKa = 11.84RR135 pKa = 11.84RR136 pKa = 11.84GIWAQRR142 pKa = 11.84DD143 pKa = 3.6NTPFGSGNPYY153 pKa = 10.58SGGQLDD159 pKa = 3.95KK160 pKa = 11.17LLRR163 pKa = 11.84DD164 pKa = 3.48HH165 pKa = 7.11SFVPEE170 pKa = 3.97AWRR173 pKa = 11.84DD174 pKa = 3.74GLFLPPFQSSLVLKK188 pKa = 7.83STRR191 pKa = 11.84FFEE194 pKa = 4.35RR195 pKa = 11.84VGRR198 pKa = 11.84LFGPAMSGVICVRR211 pKa = 11.84ARR213 pKa = 11.84KK214 pKa = 8.57EE215 pKa = 3.76AFPAVPRR222 pKa = 11.84RR223 pKa = 11.84KK224 pKa = 9.36RR225 pKa = 11.84EE226 pKa = 3.69EE227 pKa = 3.76RR228 pKa = 11.84FVRR231 pKa = 11.84VPGLSTATARR241 pKa = 11.84SSWSKK246 pKa = 11.0GRR248 pKa = 11.84FPLSRR253 pKa = 11.84EE254 pKa = 3.88

Molecular weight: 28.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3690

0

3690

1134231

41

1832

307.4

33.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.62 ± 0.058

0.669 ± 0.011

5.834 ± 0.03

5.511 ± 0.038

3.774 ± 0.029

8.672 ± 0.043

1.994 ± 0.019

5.537 ± 0.03

3.079 ± 0.034

10.286 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.595 ± 0.019

2.723 ± 0.024

5.023 ± 0.031

3.167 ± 0.021

6.569 ± 0.041

5.31 ± 0.026

5.45 ± 0.026

7.602 ± 0.032

1.313 ± 0.018

2.271 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski