Marinomonas ushuaiensis DSM 15871
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3075 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X7E135|X7E135_9GAMM Probable membrane transporter protein OS=Marinomonas ushuaiensis DSM 15871 OX=1122207 GN=MUS1_06970 PE=3 SV=1
MM1 pKa = 7.56 KK2 pKa = 10.43 SAFALSTVLLTSAAHH17 pKa = 7.15 ADD19 pKa = 3.66 FLGANAEE26 pKa = 4.06 AGIFDD31 pKa = 4.38 SSDD34 pKa = 2.86 GSATYY39 pKa = 10.81 ASLDD43 pKa = 3.44 IQHH46 pKa = 7.8 PIPLIPNLRR55 pKa = 11.84 MDD57 pKa = 3.18 VWDD60 pKa = 4.78 FEE62 pKa = 5.0 SDD64 pKa = 3.3 PSGTDD69 pKa = 2.81 ISHH72 pKa = 7.45 LDD74 pKa = 3.19 FTGYY78 pKa = 10.73 YY79 pKa = 10.46 GVGLLWASIEE89 pKa = 4.19 GGLTFRR95 pKa = 11.84 NLDD98 pKa = 3.52 MTQGSSSDD106 pKa = 3.91 SEE108 pKa = 4.52 SVPMLFLAASLAIPGTGITLAAEE131 pKa = 4.45 SKK133 pKa = 10.54 SISSFDD139 pKa = 3.41 SVTISDD145 pKa = 3.52 QSFKK149 pKa = 10.6 IQYY152 pKa = 9.56 QPIPVLGLEE161 pKa = 3.96 AGYY164 pKa = 10.7 RR165 pKa = 11.84 SIDD168 pKa = 3.38 QEE170 pKa = 4.75 TNFLGNIDD178 pKa = 3.63 YY179 pKa = 10.74 DD180 pKa = 4.26 GYY182 pKa = 11.06 FIGVTIDD189 pKa = 2.8 II190 pKa = 4.79
Molecular weight: 20.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.795
IPC_protein 3.795
Toseland 3.567
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.757
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.63
EMBOSS 3.77
Sillero 3.923
Patrickios 1.901
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|X7E668|X7E668_9GAMM DNA topoisomerase I OS=Marinomonas ushuaiensis DSM 15871 OX=1122207 GN=MUS1_09815 PE=4 SV=1
MM1 pKa = 7.2 QFNSHH6 pKa = 5.01 EE7 pKa = 4.04 QSRR10 pKa = 11.84 ILEE13 pKa = 4.33 MAWEE17 pKa = 4.29 DD18 pKa = 3.49 RR19 pKa = 11.84 TPFEE23 pKa = 5.19 AIEE26 pKa = 4.1 LQFSLNEE33 pKa = 3.75 QAVIKK38 pKa = 10.34 LMRR41 pKa = 11.84 RR42 pKa = 11.84 SLKK45 pKa = 10.61 SSSFRR50 pKa = 11.84 LWRR53 pKa = 11.84 ARR55 pKa = 11.84 VNGRR59 pKa = 11.84 KK60 pKa = 7.48 TKK62 pKa = 10.56 HH63 pKa = 5.41 LMLRR67 pKa = 11.84 SSDD70 pKa = 4.04 VIRR73 pKa = 11.84 GYY75 pKa = 11.05 CPTQYY80 pKa = 10.85 KK81 pKa = 9.93 PRR83 pKa = 5.01
Molecular weight: 9.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.663
IPC_protein 10.54
Toseland 10.804
ProMoST 10.935
Dawson 10.877
Bjellqvist 10.599
Wikipedia 11.111
Rodwell 11.052
Grimsley 10.921
Solomon 11.038
Lehninger 11.008
Nozaki 10.789
DTASelect 10.599
Thurlkill 10.789
EMBOSS 11.213
Sillero 10.818
Patrickios 10.818
IPC_peptide 11.038
IPC2_peptide 9.604
IPC2.peptide.svr19 8.817
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3075
0
3075
976217
29
2097
317.5
35.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.408 ± 0.039
0.985 ± 0.014
5.538 ± 0.032
6.354 ± 0.043
4.277 ± 0.031
6.822 ± 0.037
2.13 ± 0.022
6.753 ± 0.036
5.406 ± 0.04
10.645 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.669 ± 0.02
4.154 ± 0.03
3.888 ± 0.025
4.075 ± 0.032
4.49 ± 0.036
6.976 ± 0.039
5.352 ± 0.032
7.019 ± 0.039
1.196 ± 0.018
2.863 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here