Candidatus Altiarchaeales archaeon WOR_SM1_SCG
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2164 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D2RA85|A0A1D2RA85_9ARCH INTEIN_C_TER domain-containing protein (Fragment) OS=Candidatus Altiarchaeales archaeon WOR_SM1_SCG OX=1849261 GN=BEH94_04500 PE=3 SV=1
MM1 pKa = 6.85 KK2 pKa = 9.63 TSNIILLIFVFEE14 pKa = 4.97 IILSNLCASEE24 pKa = 3.88 EE25 pKa = 4.0 AGLVGEE31 pKa = 4.08 WHH33 pKa = 6.85 FNEE36 pKa = 4.03 TDD38 pKa = 3.15 GNIAYY43 pKa = 9.64 DD44 pKa = 3.87 FSGNGNDD51 pKa = 3.74 GTINGASWVGGVSGSALSFDD71 pKa = 3.66 GNDD74 pKa = 3.47 YY75 pKa = 11.57 VNTNLHH81 pKa = 6.53 LPQGSKK87 pKa = 8.3 TVEE90 pKa = 3.26 AWIKK94 pKa = 11.01 GFGTTYY100 pKa = 9.75 STEE103 pKa = 3.97 CPIAGEE109 pKa = 4.12 RR110 pKa = 11.84 WYY112 pKa = 10.88 SAAGGGEE119 pKa = 3.84 QLNLGVKK126 pKa = 8.71 TDD128 pKa = 3.94 EE129 pKa = 4.17 SAMLYY134 pKa = 10.29 VYY136 pKa = 10.67 SEE138 pKa = 4.57 DD139 pKa = 3.6 KK140 pKa = 11.16 GSGVNAVSDD149 pKa = 4.67 SNILDD154 pKa = 3.36 QTKK157 pKa = 7.77 WHH159 pKa = 7.06 HH160 pKa = 5.83 IAGTYY165 pKa = 9.21 EE166 pKa = 3.95 VGDD169 pKa = 3.6 TDD171 pKa = 4.51 AVLKK175 pKa = 10.49 IYY177 pKa = 10.32 IDD179 pKa = 3.77 GTLHH183 pKa = 6.27 DD184 pKa = 4.65 TVHH187 pKa = 6.5 GPIDD191 pKa = 3.78 IDD193 pKa = 3.67 NHH195 pKa = 5.4 ISTYY199 pKa = 10.7 LIGVWRR205 pKa = 11.84 RR206 pKa = 11.84 GTGAVGVRR214 pKa = 11.84 YY215 pKa = 9.22 FKK217 pKa = 11.17 GVIDD221 pKa = 3.79 EE222 pKa = 4.0 VRR224 pKa = 11.84 IYY226 pKa = 11.17 NRR228 pKa = 11.84 ALTATEE234 pKa = 3.53 ISDD237 pKa = 4.47 RR238 pKa = 11.84 YY239 pKa = 8.55 YY240 pKa = 10.32 QQDD243 pKa = 3.29 MDD245 pKa = 4.45 RR246 pKa = 11.84 DD247 pKa = 3.85 GVLNINDD254 pKa = 3.54 QCPNTTAKK262 pKa = 10.82 CEE264 pKa = 4.07 VDD266 pKa = 4.28 SSGCPLDD273 pKa = 4.05 SDD275 pKa = 5.01 NDD277 pKa = 4.15 NICNGIDD284 pKa = 3.45 TCPNTPLNCNVSSTGCKK301 pKa = 8.95 IDD303 pKa = 3.53 SDD305 pKa = 4.43 SDD307 pKa = 4.33 GVCNGVDD314 pKa = 3.47 PCPNDD319 pKa = 5.64 LEE321 pKa = 4.66 DD322 pKa = 4.48 CCTATTDD329 pKa = 3.54 TDD331 pKa = 3.53 NDD333 pKa = 3.95 SLQEE337 pKa = 4.27 SNSNCPNYY345 pKa = 10.46 DD346 pKa = 3.6 SYY348 pKa = 12.06 PNDD351 pKa = 3.56 HH352 pKa = 7.51 DD353 pKa = 3.99 NDD355 pKa = 4.31 GVVSSVDD362 pKa = 4.0 CDD364 pKa = 4.46 DD365 pKa = 4.6 SDD367 pKa = 5.0 AGNTKK372 pKa = 9.58 TKK374 pKa = 10.67 GDD376 pKa = 3.91 SDD378 pKa = 3.98 NDD380 pKa = 4.06 GVDD383 pKa = 3.34 NCIDD387 pKa = 3.68 SCPNDD392 pKa = 3.64 AGNQCGCVDD401 pKa = 3.97 HH402 pKa = 7.47 DD403 pKa = 4.73 GDD405 pKa = 4.84 GLGEE409 pKa = 4.19 GAGCRR414 pKa = 11.84 DD415 pKa = 3.66 PCSNDD420 pKa = 3.27 KK421 pKa = 11.11 DD422 pKa = 4.09 NCCTAITDD430 pKa = 3.71 TDD432 pKa = 3.62 NDD434 pKa = 3.92 GLQEE438 pKa = 4.28 NNVNCPNYY446 pKa = 10.6 DD447 pKa = 3.35 NYY449 pKa = 11.47 LNDD452 pKa = 3.84 HH453 pKa = 7.27 DD454 pKa = 4.59 NDD456 pKa = 4.26 GVISSVDD463 pKa = 3.78 CDD465 pKa = 5.12 DD466 pKa = 4.71 SDD468 pKa = 5.0 AGNTKK473 pKa = 9.56 TKK475 pKa = 10.56 GDD477 pKa = 3.81 SDD479 pKa = 4.19 GDD481 pKa = 4.01 GLNNCQDD488 pKa = 3.67 SCPDD492 pKa = 4.03 DD493 pKa = 5.42 ANNQCGCVDD502 pKa = 3.53 NDD504 pKa = 3.78 GDD506 pKa = 4.38 GLGEE510 pKa = 4.19 GTGCADD516 pKa = 3.83 PCPNDD521 pKa = 4.79 RR522 pKa = 11.84 DD523 pKa = 3.95 NCCIATTDD531 pKa = 3.76 ADD533 pKa = 3.77 NDD535 pKa = 3.55 NLQEE539 pKa = 4.27 NNQDD543 pKa = 3.41 CPNYY547 pKa = 10.58 DD548 pKa = 3.55 NYY550 pKa = 11.48 LNDD553 pKa = 3.88 HH554 pKa = 7.24 DD555 pKa = 4.82 NDD557 pKa = 4.19 GVTTLMDD564 pKa = 5.18 CDD566 pKa = 5.76 DD567 pKa = 4.41 SDD569 pKa = 4.72 ADD571 pKa = 3.73 NVKK574 pKa = 10.34 IKK576 pKa = 10.62 GDD578 pKa = 4.08 CGDD581 pKa = 3.5 QEE583 pKa = 4.53 QIPVVNVTVVNEE595 pKa = 3.73 ITVINTITIANEE607 pKa = 4.3 TIFSAVTTINEE618 pKa = 3.73 TRR620 pKa = 11.84 RR621 pKa = 11.84 YY622 pKa = 9.63 LNVSEE627 pKa = 4.21 EE628 pKa = 4.27 VNLEE632 pKa = 3.85 VEE634 pKa = 4.53 EE635 pKa = 4.57 KK636 pKa = 10.23 IKK638 pKa = 10.65 EE639 pKa = 4.07 VEE641 pKa = 4.16 DD642 pKa = 4.13 LIEE645 pKa = 4.07 RR646 pKa = 11.84 AKK648 pKa = 10.98 EE649 pKa = 3.87 KK650 pKa = 10.47 IALANSLINTDD661 pKa = 3.74 PKK663 pKa = 10.82 RR664 pKa = 11.84 AMDD667 pKa = 3.58 EE668 pKa = 4.16 AEE670 pKa = 4.37 EE671 pKa = 4.28 SNEE674 pKa = 3.73 LAEE677 pKa = 4.32 RR678 pKa = 11.84 AVKK681 pKa = 9.07 LTMEE685 pKa = 4.52 LSKK688 pKa = 11.04 NYY690 pKa = 7.99 KK691 pKa = 8.64 TKK693 pKa = 10.91 SSVLPATSQSDD704 pKa = 3.54 ALVSEE709 pKa = 4.62 TEE711 pKa = 4.24 EE712 pKa = 4.23 FSGDD716 pKa = 3.25 TFKK719 pKa = 9.31 WTGIAGIVCFIILTGILIFAARR741 pKa = 11.84 KK742 pKa = 9.59 KK743 pKa = 10.24 YY744 pKa = 10.59
Molecular weight: 80.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.783
IPC_protein 3.834
Toseland 3.605
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.656
Grimsley 3.503
Solomon 3.834
Lehninger 3.783
Nozaki 3.935
DTASelect 4.215
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.961
Patrickios 0.642
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A1D2QUD6|A0A1D2QUD6_9ARCH PQQ_3 domain-containing protein OS=Candidatus Altiarchaeales archaeon WOR_SM1_SCG OX=1849261 GN=BEH94_01960 PE=4 SV=1
MM1 pKa = 7.26 YY2 pKa = 10.79 SHH4 pKa = 6.87 TKK6 pKa = 9.9 KK7 pKa = 10.34 VGSIGRR13 pKa = 11.84 YY14 pKa = 7.07 GPRR17 pKa = 11.84 VGRR20 pKa = 11.84 KK21 pKa = 7.36 VRR23 pKa = 11.84 MAMRR27 pKa = 11.84 KK28 pKa = 9.03 IEE30 pKa = 4.51 DD31 pKa = 3.46 KK32 pKa = 10.64 TRR34 pKa = 11.84 EE35 pKa = 4.0 ANKK38 pKa = 10.17 CKK40 pKa = 10.62 SCGKK44 pKa = 9.26 PKK46 pKa = 9.9 IRR48 pKa = 11.84 RR49 pKa = 11.84 EE50 pKa = 3.77 SAGIWKK56 pKa = 9.46 CRR58 pKa = 11.84 ACGVTFTGGAHH69 pKa = 6.47 LPVTEE74 pKa = 4.27 RR75 pKa = 11.84 RR76 pKa = 3.73
Molecular weight: 8.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.321
IPC2_protein 9.823
IPC_protein 10.599
Toseland 11.052
ProMoST 11.052
Dawson 11.096
Bjellqvist 10.804
Wikipedia 11.316
Rodwell 11.389
Grimsley 11.125
Solomon 11.272
Lehninger 11.228
Nozaki 11.038
DTASelect 10.804
Thurlkill 11.023
EMBOSS 11.447
Sillero 11.038
Patrickios 11.125
IPC_peptide 11.272
IPC2_peptide 9.984
IPC2.peptide.svr19 8.717
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2164
0
2164
656850
48
5438
303.5
34.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.755 ± 0.058
1.631 ± 0.052
5.798 ± 0.053
7.891 ± 0.071
4.31 ± 0.049
6.904 ± 0.052
1.629 ± 0.022
9.204 ± 0.054
8.965 ± 0.103
8.459 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.274 ± 0.028
5.937 ± 0.115
3.401 ± 0.034
2.024 ± 0.026
3.914 ± 0.049
5.869 ± 0.057
5.085 ± 0.082
6.252 ± 0.049
0.958 ± 0.02
3.738 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here