Marinospirillum celere
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2818 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I1G398|A0A1I1G398_9GAMM Ribosome-associated protein OS=Marinospirillum celere OX=1122252 GN=SAMN05660443_1256 PE=4 SV=1
MM1 pKa = 7.57 KK2 pKa = 10.38 KK3 pKa = 10.61 LNLALLISLGWLLTACLAEE22 pKa = 3.96 QDD24 pKa = 3.6 EE25 pKa = 4.86 SSRR28 pKa = 11.84 QLGSDD33 pKa = 3.43 ASQGGEE39 pKa = 3.79 YY40 pKa = 10.22 RR41 pKa = 11.84 QGEE44 pKa = 4.11 GTARR48 pKa = 11.84 VEE50 pKa = 4.58 FSVHH54 pKa = 5.82 LPGDD58 pKa = 3.57 ASAAYY63 pKa = 9.35 IDD65 pKa = 4.04 TEE67 pKa = 4.16 NTRR70 pKa = 11.84 SIVVSVFPSSIAGFDD85 pKa = 3.28 VDD87 pKa = 5.06 RR88 pKa = 11.84 FQATDD93 pKa = 3.05 VLRR96 pKa = 11.84 AYY98 pKa = 7.3 FQCVMANAPSEE109 pKa = 3.99 GDD111 pKa = 3.17 MGIQEE116 pKa = 4.45 MEE118 pKa = 4.58 SACGRR123 pKa = 11.84 YY124 pKa = 8.15 PAQIADD130 pKa = 3.92 TPLYY134 pKa = 10.61 SATLTSDD141 pKa = 3.93 LPAAGFNLQPGNSYY155 pKa = 10.34 IVLAAQYY162 pKa = 10.5 SSSNPAGLTPVGWTTTTITPNDD184 pKa = 3.67 GNNDD188 pKa = 3.2 VEE190 pKa = 5.28 LNLLYY195 pKa = 10.72 GSWEE199 pKa = 3.94 FDD201 pKa = 3.12 GGPSLQLLNRR211 pKa = 11.84 STYY214 pKa = 11.31 AFGNFIGFNEE224 pKa = 3.93 EE225 pKa = 3.8 DD226 pKa = 3.45 LEE228 pKa = 4.49 EE229 pKa = 4.2 FTEE232 pKa = 5.59 APDD235 pKa = 2.86 WWPASTHH242 pKa = 5.69 VMSTPAMALGLADD255 pKa = 4.23 TGDD258 pKa = 3.34 ATINLDD264 pKa = 3.67 RR265 pKa = 11.84 LHH267 pKa = 6.51 LRR269 pKa = 11.84 SYY271 pKa = 11.62 YY272 pKa = 10.14 EE273 pKa = 4.0 LSPNNQVDD281 pKa = 3.65 SARR284 pKa = 11.84 NDD286 pKa = 3.03 EE287 pKa = 4.52 GIRR290 pKa = 11.84 LFGAQGEE297 pKa = 4.37 PDD299 pKa = 3.55 NFDD302 pKa = 5.13 LFGQYY307 pKa = 9.43 PWGILAPHH315 pKa = 6.76 EE316 pKa = 4.69 PLLQSSVAGNAFFVEE331 pKa = 4.38 PRR333 pKa = 11.84 FEE335 pKa = 4.13 LVEE338 pKa = 5.09 GIDD341 pKa = 4.74 DD342 pKa = 5.65 DD343 pKa = 4.78 PDD345 pKa = 4.25 LMLGRR350 pKa = 11.84 GVYY353 pKa = 10.01 EE354 pKa = 4.57 PATLMQQYY362 pKa = 10.66 APEE365 pKa = 3.73 QSQANRR371 pKa = 11.84 HH372 pKa = 5.13 RR373 pKa = 11.84 LGLGTWEE380 pKa = 3.96 VTIGEE385 pKa = 4.52 EE386 pKa = 3.74 ISTEE390 pKa = 3.93 NVEE393 pKa = 4.07 IFAGLMLVSGEE404 pKa = 3.92 NDD406 pKa = 2.9 ILNLLRR412 pKa = 11.84 DD413 pKa = 3.58 ATEE416 pKa = 3.77 EE417 pKa = 4.25 VEE419 pKa = 4.41 GQRR422 pKa = 11.84 SFDD425 pKa = 3.43 QLNVRR430 pKa = 11.84 RR431 pKa = 11.84 PVFDD435 pKa = 3.3 QFGQFGQFGQFGQFGQFGQPVPEE458 pKa = 4.28 VLPEE462 pKa = 4.26 SNLTIAFFDD471 pKa = 3.86 IEE473 pKa = 4.47 TQQGEE478 pKa = 4.35 LLIAEE483 pKa = 4.64 GTDD486 pKa = 3.36 TQNFQALDD494 pKa = 3.47 ALEE497 pKa = 4.21 PTRR500 pKa = 11.84 LTAADD505 pKa = 5.37 RR506 pKa = 11.84 IQGSLIEE513 pKa = 4.13 YY514 pKa = 8.41 VGYY517 pKa = 10.87 YY518 pKa = 9.83 NAIEE522 pKa = 4.35 SPPYY526 pKa = 10.08 QYY528 pKa = 11.22 PLLLEE533 pKa = 4.79 DD534 pKa = 3.98 EE535 pKa = 5.02 EE536 pKa = 6.06 GNFTQVDD543 pKa = 4.42 TIEE546 pKa = 4.14 VNPVIGGVAASSSLQNTSGSSLRR569 pKa = 11.84 SSSQALILRR578 pKa = 11.84 NLQLSALAADD588 pKa = 4.11 SGLIASTAASGCFVMDD604 pKa = 4.91 LDD606 pKa = 4.13 VQRR609 pKa = 11.84 VSSFYY614 pKa = 10.93 LFNPDD619 pKa = 3.17 EE620 pKa = 4.47 EE621 pKa = 4.79 TWLPAAPEE629 pKa = 3.79 FGYY632 pKa = 9.74 WYY634 pKa = 9.74 QAEE637 pKa = 4.45 DD638 pKa = 3.4 GSYY641 pKa = 11.01 LRR643 pKa = 11.84 DD644 pKa = 3.41 EE645 pKa = 4.46 EE646 pKa = 4.97 GKK648 pKa = 9.9 IEE650 pKa = 4.16 TFGTGASPSEE660 pKa = 4.0 TLEE663 pKa = 3.98 EE664 pKa = 4.63 DD665 pKa = 3.65 GVIYY669 pKa = 10.28 SIQPSGGMTGIIDD682 pKa = 4.15 ASTDD686 pKa = 3.53 EE687 pKa = 5.34 SDD689 pKa = 4.81 LEE691 pKa = 4.23 QNFYY695 pKa = 10.95 AWSEE699 pKa = 4.04 EE700 pKa = 3.92 GSGKK704 pKa = 10.08 VEE706 pKa = 4.01 VCIQPVSLTAGPYY719 pKa = 10.5 SNPEE723 pKa = 3.77 YY724 pKa = 10.35 NAEE727 pKa = 3.97 SDD729 pKa = 4.4 VVIQQ733 pKa = 4.44
Molecular weight: 79.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.617
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.668
Rodwell 3.643
Grimsley 3.528
Solomon 3.783
Lehninger 3.732
Nozaki 3.884
DTASelect 4.075
Thurlkill 3.643
EMBOSS 3.694
Sillero 3.935
Patrickios 1.469
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|A0A1I1IAE0|A0A1I1IAE0_9GAMM Sulfur carrier protein OS=Marinospirillum celere OX=1122252 GN=SAMN05660443_2299 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.3 NGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.62 GRR39 pKa = 11.84 KK40 pKa = 9.11 RR41 pKa = 11.84 ISAA44 pKa = 3.72
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2818
0
2818
946962
37
5359
336.0
37.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.535 ± 0.051
0.87 ± 0.015
5.301 ± 0.039
7.06 ± 0.051
3.728 ± 0.031
7.125 ± 0.058
2.163 ± 0.021
4.912 ± 0.039
4.225 ± 0.046
12.509 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.279 ± 0.026
3.255 ± 0.04
4.533 ± 0.04
5.63 ± 0.056
5.757 ± 0.052
5.86 ± 0.039
4.874 ± 0.07
6.363 ± 0.04
1.467 ± 0.024
2.553 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here