Tipula oleracea nudivirus
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 131 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4VFK1|A0A0B4VFK1_9VIRU TK3 OS=Tipula oleracea nudivirus OX=1546257 GN=tk3 PE=4 SV=1
MM1 pKa = 7.69 AFLISEE7 pKa = 4.02 PAGRR11 pKa = 11.84 RR12 pKa = 11.84 FEE14 pKa = 4.24 EE15 pKa = 5.26 LYY17 pKa = 10.28 GDD19 pKa = 4.45 MYY21 pKa = 11.46 RR22 pKa = 11.84 EE23 pKa = 4.12 VTKK26 pKa = 10.68 LANFYY31 pKa = 10.75 SGEE34 pKa = 4.13 DD35 pKa = 3.52 VVDD38 pKa = 3.97 NSVLYY43 pKa = 9.73 PMIEE47 pKa = 3.85 ISDD50 pKa = 4.19 DD51 pKa = 3.48 EE52 pKa = 4.74 SSIDD56 pKa = 3.79 EE57 pKa = 4.81 DD58 pKa = 5.41 GEE60 pKa = 4.39 DD61 pKa = 3.7 AVDD64 pKa = 4.08 NSVLYY69 pKa = 9.76 PMIEE73 pKa = 3.85 ISDD76 pKa = 4.19 DD77 pKa = 3.48 EE78 pKa = 4.74 SSIDD82 pKa = 3.72 EE83 pKa = 5.09 DD84 pKa = 5.11 GVIPDD89 pKa = 3.89 QLMDD93 pKa = 5.51 DD94 pKa = 4.63 MSDD97 pKa = 4.32 CDD99 pKa = 5.66 DD100 pKa = 5.08 DD101 pKa = 6.52 DD102 pKa = 5.05 ISEE105 pKa = 4.8 GDD107 pKa = 4.44 DD108 pKa = 4.11 DD109 pKa = 6.94 DD110 pKa = 7.11 DD111 pKa = 4.53 MSEE114 pKa = 5.36 DD115 pKa = 3.82 DD116 pKa = 4.13 GKK118 pKa = 10.75 NNYY121 pKa = 8.28 MEE123 pKa = 3.96 IHH125 pKa = 6.31 SKK127 pKa = 10.43 FKK129 pKa = 10.96 EE130 pKa = 3.77 FLTYY134 pKa = 10.77 NVLKK138 pKa = 10.68 NIRR141 pKa = 11.84 TMNGNIYY148 pKa = 10.71 SFISRR153 pKa = 11.84 NNYY156 pKa = 8.45 YY157 pKa = 10.4 VSEE160 pKa = 4.09 LMRR163 pKa = 11.84 NVVKK167 pKa = 10.76 DD168 pKa = 4.28 DD169 pKa = 3.25 IQFEE173 pKa = 4.5 IKK175 pKa = 10.43 FKK177 pKa = 11.07 INFKK181 pKa = 9.76 TYY183 pKa = 9.79 HH184 pKa = 6.88 LLTIGSSLPNNIMNDD199 pKa = 3.17 NWEE202 pKa = 4.11 KK203 pKa = 10.99 LQVHH207 pKa = 6.91 KK208 pKa = 10.73 KK209 pKa = 8.92 NQIEE213 pKa = 4.21 CRR215 pKa = 11.84 VKK217 pKa = 10.26 NVKK220 pKa = 10.09 NVIFTDD226 pKa = 3.38 KK227 pKa = 11.05 SMSFFSHH234 pKa = 6.51 VGRR237 pKa = 11.84 VHH239 pKa = 7.07 WNPEE243 pKa = 3.77 LKK245 pKa = 10.24 VAYY248 pKa = 10.01 SLTIPIEE255 pKa = 4.17 EE256 pKa = 4.59 PPEE259 pKa = 3.84 KK260 pKa = 10.48 LKK262 pKa = 11.1 NQIFKK267 pKa = 9.87 NQKK270 pKa = 8.71 TISSLNFEE278 pKa = 4.57 CFEE281 pKa = 3.95 AAEE284 pKa = 4.37 YY285 pKa = 10.0 PFNLNMYY292 pKa = 8.82 FVKK295 pKa = 10.03 TYY297 pKa = 9.51 NFKK300 pKa = 10.52 IIQCIQNDD308 pKa = 3.71 DD309 pKa = 3.48 VEE311 pKa = 4.53 YY312 pKa = 10.96 NITFEE317 pKa = 4.17 ICNGLNDD324 pKa = 5.53 DD325 pKa = 4.54 SLTTKK330 pKa = 10.64 KK331 pKa = 8.84 ITEE334 pKa = 4.49 IIADD338 pKa = 3.79 VKK340 pKa = 11.29 SNWNSFNLNGIIEE353 pKa = 4.46 TKK355 pKa = 9.47 LYY357 pKa = 10.23 KK358 pKa = 10.02 YY359 pKa = 9.97 RR360 pKa = 11.84 VYY362 pKa = 10.82 NEE364 pKa = 4.69 HH365 pKa = 6.92 EE366 pKa = 4.59 VTPLPQIGFKK376 pKa = 10.77 DD377 pKa = 3.52 AVIALNDD384 pKa = 3.35 IYY386 pKa = 11.26 DD387 pKa = 3.74 YY388 pKa = 11.91
Molecular weight: 45.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.307
IPC2_protein 4.291
IPC_protein 4.279
Toseland 4.088
ProMoST 4.406
Dawson 4.24
Bjellqvist 4.393
Wikipedia 4.139
Rodwell 4.113
Grimsley 3.999
Solomon 4.24
Lehninger 4.202
Nozaki 4.355
DTASelect 4.558
Thurlkill 4.113
EMBOSS 4.151
Sillero 4.393
Patrickios 3.49
IPC_peptide 4.24
IPC2_peptide 4.38
IPC2.peptide.svr19 4.321
Protein with the highest isoelectric point:
>tr|A0A0B4VG75|A0A0B4VG75_9VIRU Uncharacterized protein OS=Tipula oleracea nudivirus OX=1546257 GN=TONV_061 PE=4 SV=1
MM1 pKa = 7.53 KK2 pKa = 9.94 IIHH5 pKa = 6.13 FSFYY9 pKa = 10.47 KK10 pKa = 10.41 SYY12 pKa = 10.33 ISLYY16 pKa = 10.13 IYY18 pKa = 10.16 IRR20 pKa = 11.84 NKK22 pKa = 10.56 NEE24 pKa = 3.37 NKK26 pKa = 9.47 TYY28 pKa = 10.79 RR29 pKa = 11.84 SFLLIKK35 pKa = 10.27 KK36 pKa = 8.41 MPYY39 pKa = 9.75 HH40 pKa = 6.07 EE41 pKa = 4.91 LEE43 pKa = 4.44 PYY45 pKa = 9.27 EE46 pKa = 4.3 RR47 pKa = 11.84 SRR49 pKa = 11.84 SRR51 pKa = 11.84 SHH53 pKa = 6.54 SRR55 pKa = 11.84 SHH57 pKa = 6.79 LSGGRR62 pKa = 11.84 TRR64 pKa = 11.84 ARR66 pKa = 11.84 SRR68 pKa = 11.84 SGSRR72 pKa = 11.84 SGGARR77 pKa = 11.84 RR78 pKa = 11.84 RR79 pKa = 11.84 SGSRR83 pKa = 11.84 RR84 pKa = 11.84 RR85 pKa = 11.84 SRR87 pKa = 11.84 SRR89 pKa = 11.84 SGSRR93 pKa = 11.84 SRR95 pKa = 11.84 GAVGQRR101 pKa = 11.84 RR102 pKa = 11.84 RR103 pKa = 11.84 RR104 pKa = 11.84 RR105 pKa = 11.84 GGRR108 pKa = 11.84 RR109 pKa = 11.84 RR110 pKa = 11.84 SRR112 pKa = 11.84 SRR114 pKa = 11.84 SRR116 pKa = 11.84 SRR118 pKa = 11.84 YY119 pKa = 8.61
Molecular weight: 14.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.23
IPC2_protein 10.847
IPC_protein 12.047
Toseland 12.193
ProMoST 12.691
Dawson 12.193
Bjellqvist 12.193
Wikipedia 12.676
Rodwell 11.813
Grimsley 12.237
Solomon 12.691
Lehninger 12.588
Nozaki 12.193
DTASelect 12.193
Thurlkill 12.193
EMBOSS 12.691
Sillero 12.193
Patrickios 11.52
IPC_peptide 12.691
IPC2_peptide 11.681
IPC2.peptide.svr19 9.134
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
131
0
131
41473
36
1380
316.6
36.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.505 ± 0.107
1.681 ± 0.105
6.071 ± 0.155
5.893 ± 0.199
5.17 ± 0.161
2.452 ± 0.107
1.854 ± 0.086
10.952 ± 0.182
8.953 ± 0.215
8.813 ± 0.167
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.105 ± 0.077
10.375 ± 0.241
3.2 ± 0.146
3.532 ± 0.163
2.696 ± 0.112
7.099 ± 0.137
5.9 ± 0.133
4.63 ± 0.114
0.422 ± 0.04
5.698 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here