Moraxella phage Mcat3
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6PGS5|A0A0R6PGS5_9CAUD Uncharacterized protein OS=Moraxella phage Mcat3 OX=1647546 PE=4 SV=1
MM1 pKa = 7.73 NDD3 pKa = 3.05 ACDD6 pKa = 3.41 TAEE9 pKa = 4.82 LNNPADD15 pKa = 3.92 GQGFVYY21 pKa = 9.74 PVIDD25 pKa = 4.16 GEE27 pKa = 4.55 CKK29 pKa = 9.85 IMRR32 pKa = 11.84 YY33 pKa = 10.31 DD34 pKa = 3.6 DD35 pKa = 4.58 FIKK38 pKa = 10.34 EE39 pKa = 4.06 FCFLPKK45 pKa = 10.3 DD46 pKa = 3.21 IRR48 pKa = 11.84 LNDD51 pKa = 3.47 EE52 pKa = 3.85
Molecular weight: 5.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.057
IPC2_protein 3.999
IPC_protein 3.897
Toseland 3.694
ProMoST 4.024
Dawson 3.91
Bjellqvist 4.151
Wikipedia 3.872
Rodwell 3.732
Grimsley 3.605
Solomon 3.884
Lehninger 3.846
Nozaki 4.037
DTASelect 4.266
Thurlkill 3.77
EMBOSS 3.872
Sillero 4.024
Patrickios 3.121
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.968
Protein with the highest isoelectric point:
>tr|A0A0R6PH02|A0A0R6PH02_9CAUD Tail protein OS=Moraxella phage Mcat3 OX=1647546 PE=4 SV=1
MM1 pKa = 7.7 AKK3 pKa = 10.25 LGNLPPRR10 pKa = 11.84 LKK12 pKa = 9.39 PTQSHH17 pKa = 4.99 TPKK20 pKa = 10.42 KK21 pKa = 10.01 KK22 pKa = 8.53 WGQARR27 pKa = 11.84 GGRR30 pKa = 11.84 WWRR33 pKa = 11.84 RR34 pKa = 11.84 LRR36 pKa = 11.84 AEE38 pKa = 3.9 FLARR42 pKa = 11.84 NNYY45 pKa = 7.55 TCQLCGRR52 pKa = 11.84 VGGRR56 pKa = 11.84 LEE58 pKa = 4.88 LDD60 pKa = 3.8 HH61 pKa = 6.99 IVNKK65 pKa = 10.57 AVGGTDD71 pKa = 4.2 DD72 pKa = 4.03 KK73 pKa = 11.72 TNLQILCYY81 pKa = 9.44 QCHH84 pKa = 4.95 KK85 pKa = 9.73 TKK87 pKa = 9.37 TQSEE91 pKa = 4.72 SKK93 pKa = 10.7 AGGVLQFLGG102 pKa = 3.78
Molecular weight: 11.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.272
IPC2_protein 9.531
IPC_protein 9.663
Toseland 10.599
ProMoST 10.116
Dawson 10.687
Bjellqvist 10.306
Wikipedia 10.804
Rodwell 11.184
Grimsley 10.701
Solomon 10.73
Lehninger 10.716
Nozaki 10.599
DTASelect 10.292
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.599
Patrickios 10.935
IPC_peptide 10.745
IPC2_peptide 9.268
IPC2.peptide.svr19 8.484
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
40
0
40
8759
39
1175
219.0
24.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.825 ± 0.662
0.982 ± 0.179
6.508 ± 0.228
5.674 ± 0.342
3.539 ± 0.236
6.359 ± 0.31
2.455 ± 0.217
6.77 ± 0.294
7.455 ± 0.247
8.882 ± 0.352
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.569 ± 0.172
4.978 ± 0.307
3.494 ± 0.36
4.567 ± 0.267
4.361 ± 0.321
6.165 ± 0.479
6.291 ± 0.391
5.72 ± 0.315
1.119 ± 0.147
3.288 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here