Vibrio phage Athena1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5BFZ2|A0A2H5BFZ2_9CAUD Uncharacterized protein OS=Vibrio phage Athena1 OX=2060111 GN=Athena1_0008 PE=4 SV=1
MM1 pKa = 7.69VNDD4 pKa = 4.51CDD6 pKa = 3.51MPAPYY11 pKa = 9.66RR12 pKa = 11.84YY13 pKa = 8.03TPGEE17 pKa = 4.29VEE19 pKa = 3.86WMIEE23 pKa = 3.63NSIPMLRR30 pKa = 11.84WFDD33 pKa = 4.2EE34 pKa = 4.0YY35 pKa = 11.75DD36 pKa = 3.25LAYY39 pKa = 9.36PKK41 pKa = 10.48CLEE44 pKa = 4.15SAKK47 pKa = 10.91SGGDD51 pKa = 3.19QQ52 pKa = 3.85

Molecular weight: 6.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5BG01|A0A2H5BG01_9CAUD Uncharacterized protein OS=Vibrio phage Athena1 OX=2060111 GN=Athena1_0026 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 10.47KK3 pKa = 9.89EE4 pKa = 3.74FRR6 pKa = 11.84EE7 pKa = 4.5LITKK11 pKa = 10.28RR12 pKa = 11.84GHH14 pKa = 5.43NVQSFSVKK22 pKa = 10.43CGVQRR27 pKa = 11.84NTVYY31 pKa = 11.03SFGRR35 pKa = 11.84GAGGTNLSTIRR46 pKa = 11.84KK47 pKa = 7.34FAKK50 pKa = 8.86EE51 pKa = 3.91LKK53 pKa = 8.77MKK55 pKa = 10.15PSEE58 pKa = 4.41LIKK61 pKa = 11.02FMGEE65 pKa = 3.6

Molecular weight: 7.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

11707

37

788

205.4

22.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.098 ± 0.395

1.051 ± 0.135

6.398 ± 0.238

7.414 ± 0.493

4.194 ± 0.223

7.158 ± 0.408

1.555 ± 0.162

6.022 ± 0.2

7.004 ± 0.407

7.79 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.264 ± 0.136

5.159 ± 0.181

3.4 ± 0.154

3.801 ± 0.163

4.322 ± 0.251

8.081 ± 0.295

5.817 ± 0.38

6.629 ± 0.304

1.256 ± 0.152

3.588 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski