Gordonia phage Frokostdame

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345L320|A0A345L320_9CAUD Minor tail protein OS=Gordonia phage Frokostdame OX=2250320 GN=30 PE=4 SV=1
MM1 pKa = 7.15MANDD5 pKa = 4.88TIFEE9 pKa = 4.28LPEE12 pKa = 4.65IPGVTFTASYY22 pKa = 10.84GSGGEE27 pKa = 4.12TGLPSNWIRR36 pKa = 11.84IVGTVEE42 pKa = 3.91NPWYY46 pKa = 10.09EE47 pKa = 3.64PTYY50 pKa = 11.14NYY52 pKa = 11.12GLDD55 pKa = 3.93PNGHH59 pKa = 5.52TEE61 pKa = 4.34ITDD64 pKa = 2.86PWKK67 pKa = 10.66RR68 pKa = 11.84HH69 pKa = 3.79TQFPEE74 pKa = 3.86VCAMGFGGPSIGLPTDD90 pKa = 3.96PPPPPVEE97 pKa = 4.39PEE99 pKa = 3.83PTPDD103 pKa = 3.89IIEE106 pKa = 4.47EE107 pKa = 4.28PTDD110 pKa = 3.47GG111 pKa = 4.67

Molecular weight: 12.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345L346|A0A345L346_9CAUD Uncharacterized protein OS=Gordonia phage Frokostdame OX=2250320 GN=56 PE=4 SV=1
MM1 pKa = 6.98NTTAADD7 pKa = 3.36QPTAEE12 pKa = 3.97RR13 pKa = 11.84SQFAEE18 pKa = 3.47RR19 pKa = 11.84DD20 pKa = 3.5NFRR23 pKa = 11.84DD24 pKa = 3.38LTPYY28 pKa = 10.68EE29 pKa = 3.99EE30 pKa = 5.72AILHH34 pKa = 6.09GMTKK38 pKa = 10.04PAYY41 pKa = 9.22FPDD44 pKa = 3.68RR45 pKa = 11.84GYY47 pKa = 12.1VMVQGVYY54 pKa = 9.66QGYY57 pKa = 10.84ADD59 pKa = 4.44DD60 pKa = 4.87RR61 pKa = 11.84VITHH65 pKa = 7.37RR66 pKa = 11.84DD67 pKa = 3.09GTIEE71 pKa = 4.9FIPDD75 pKa = 2.97PRR77 pKa = 11.84IARR80 pKa = 11.84TEE82 pKa = 3.57RR83 pKa = 11.84RR84 pKa = 11.84RR85 pKa = 11.84AKK87 pKa = 10.44NKK89 pKa = 9.87AGRR92 pKa = 11.84KK93 pKa = 6.53SRR95 pKa = 11.84RR96 pKa = 11.84INRR99 pKa = 11.84IRR101 pKa = 11.84ARR103 pKa = 11.84RR104 pKa = 3.47

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

17002

40

1824

202.4

22.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.263 ± 0.461

1.035 ± 0.132

7.158 ± 0.329

5.823 ± 0.291

2.67 ± 0.144

8.387 ± 0.329

2.082 ± 0.167

4.435 ± 0.155

3.276 ± 0.22

7.481 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.229 ± 0.154

3.017 ± 0.158

5.829 ± 0.203

3.329 ± 0.137

7.952 ± 0.39

5.223 ± 0.201

6.864 ± 0.235

7.752 ± 0.267

1.87 ± 0.124

2.323 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski