Variovorax sp. MHTC-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Variovorax; unclassified Variovorax

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5481 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3R9YYU7|A0A3R9YYU7_9BURK Tetratricopeptide repeat protein OS=Variovorax sp. MHTC-1 OX=2495593 GN=EJI01_14815 PE=4 SV=1
MM1 pKa = 7.25SAVAEE6 pKa = 4.41NIQPQMPEE14 pKa = 4.29PIVFTDD20 pKa = 3.63SAAAKK25 pKa = 10.02VADD28 pKa = 5.45LIAEE32 pKa = 4.31EE33 pKa = 4.82GNPDD37 pKa = 2.99LKK39 pKa = 11.13LRR41 pKa = 11.84VFVQGGGCSGFQYY54 pKa = 10.94GFTFDD59 pKa = 4.81EE60 pKa = 4.64ITNEE64 pKa = 4.77DD65 pKa = 4.2DD66 pKa = 3.25TTMTKK71 pKa = 10.66NGVSLLIDD79 pKa = 3.54AMSYY83 pKa = 10.32QYY85 pKa = 11.5LVGAEE90 pKa = 3.79IDD92 pKa = 3.91YY93 pKa = 11.36KK94 pKa = 11.19EE95 pKa = 4.67DD96 pKa = 3.23LQGAQFVIKK105 pKa = 10.43NPNATSTCGCGSSFSAA121 pKa = 4.79

Molecular weight: 12.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R9YVY2|A0A3R9YVY2_9BURK (2Fe-2S)-binding protein OS=Variovorax sp. MHTC-1 OX=2495593 GN=EJI01_19200 PE=4 SV=1
MM1 pKa = 7.3RR2 pKa = 11.84QAGGTRR8 pKa = 11.84TANEE12 pKa = 3.75APVRR16 pKa = 11.84RR17 pKa = 11.84LRR19 pKa = 11.84RR20 pKa = 11.84SRR22 pKa = 11.84PPPRR26 pKa = 11.84LRR28 pKa = 11.84RR29 pKa = 11.84SSCRR33 pKa = 11.84PPRR36 pKa = 11.84PRR38 pKa = 11.84PRR40 pKa = 11.84LRR42 pKa = 11.84PRR44 pKa = 11.84LCPRR48 pKa = 11.84PSPRR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84PNPRR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84PSRR64 pKa = 11.84FRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84GPSKK72 pKa = 9.55PRR74 pKa = 11.84RR75 pKa = 11.84PRR77 pKa = 11.84GPDD80 pKa = 2.87RR81 pKa = 11.84PSPRR85 pKa = 11.84SPLHH89 pKa = 6.21PGHH92 pKa = 6.95AKK94 pKa = 9.89LRR96 pKa = 11.84RR97 pKa = 11.84PRR99 pKa = 11.84RR100 pKa = 11.84WLHH103 pKa = 5.67RR104 pKa = 11.84RR105 pKa = 11.84PLPSRR110 pKa = 11.84RR111 pKa = 11.84LHH113 pKa = 6.14RR114 pKa = 11.84SWRR117 pKa = 11.84LPRR120 pKa = 11.84WNPPHH125 pKa = 6.49LRR127 pKa = 11.84VRR129 pKa = 11.84KK130 pKa = 9.25RR131 pKa = 11.84HH132 pKa = 4.5AQARR136 pKa = 11.84ASSAARR142 pKa = 11.84AAWSRR147 pKa = 11.84SAPRR151 pKa = 11.84RR152 pKa = 11.84NSGRR156 pKa = 11.84APNARR161 pKa = 11.84PCGASSRR168 pKa = 11.84STKK171 pKa = 10.19RR172 pKa = 11.84SAIPACSTEE181 pKa = 3.77GRR183 pKa = 11.84PVRR186 pKa = 11.84RR187 pKa = 11.84RR188 pKa = 11.84PQPFF192 pKa = 2.92

Molecular weight: 22.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5481

0

5481

1763937

24

3688

321.8

34.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.369 ± 0.047

0.918 ± 0.01

5.129 ± 0.023

5.434 ± 0.034

3.547 ± 0.024

8.493 ± 0.043

2.212 ± 0.018

4.345 ± 0.025

3.171 ± 0.03

10.589 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.398 ± 0.016

2.517 ± 0.028

5.342 ± 0.029

3.669 ± 0.021

7.171 ± 0.042

5.437 ± 0.028

5.058 ± 0.041

7.583 ± 0.026

1.454 ± 0.014

2.163 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski