Citrobacter virus HCF1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Hicfunavirus

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2F3W1|A0A5Q2F3W1_9CAUD Uncharacterized protein OS=Citrobacter virus HCF1 OX=2653507 GN=HCF1_21 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.37LLCTGEE8 pKa = 4.37YY9 pKa = 10.12PGFTHH14 pKa = 7.59GEE16 pKa = 4.28MYY18 pKa = 9.22TATVGFDD25 pKa = 2.82QTFGYY30 pKa = 9.44KK31 pKa = 10.13CYY33 pKa = 10.44VVKK36 pKa = 10.83DD37 pKa = 3.78DD38 pKa = 5.68DD39 pKa = 4.72GDD41 pKa = 3.76SRR43 pKa = 11.84SIDD46 pKa = 3.61FEE48 pKa = 4.33TEE50 pKa = 3.75DD51 pKa = 4.76FIEE54 pKa = 4.85DD55 pKa = 3.49KK56 pKa = 11.25YY57 pKa = 11.72LL58 pKa = 4.14

Molecular weight: 6.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2F2M3|A0A5Q2F2M3_9CAUD Uncharacterized protein OS=Citrobacter virus HCF1 OX=2653507 GN=HCF1_03 PE=4 SV=1
MM1 pKa = 7.5IKK3 pKa = 10.15FRR5 pKa = 11.84YY6 pKa = 9.13LICTATRR13 pKa = 11.84TEE15 pKa = 4.01VARR18 pKa = 11.84ALTGLGEE25 pKa = 4.48HH26 pKa = 5.79GHH28 pKa = 6.04IVTVVKK34 pKa = 10.4RR35 pKa = 11.84KK36 pKa = 9.94SGAHH40 pKa = 5.3GSVKK44 pKa = 9.9IGMKK48 pKa = 8.34TQSHH52 pKa = 5.52SS53 pKa = 3.21

Molecular weight: 5.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

12947

37

1238

182.4

20.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.732 ± 0.352

1.699 ± 0.201

5.886 ± 0.248

6.372 ± 0.288

4.117 ± 0.187

6.928 ± 0.299

1.854 ± 0.227

6.534 ± 0.262

7.222 ± 0.353

7.229 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.958 ± 0.197

4.596 ± 0.215

3.136 ± 0.221

3.684 ± 0.269

6.001 ± 0.294

6.249 ± 0.285

5.77 ± 0.288

6.89 ± 0.262

1.444 ± 0.103

3.7 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski