Haematococcus lacustris (Green alga) (Haematococcus pluvialis)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta;

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28121 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A0ALX9|A0A6A0ALX9_HAELA Uncharacterized protein OS=Haematococcus lacustris OX=44745 GN=HaLaN_32983 PE=4 SV=1
MM1 pKa = 8.15DD2 pKa = 5.56FGSIAPPEE10 pKa = 4.16KK11 pKa = 10.32SASDD15 pKa = 3.69EE16 pKa = 3.98VDD18 pKa = 2.96ADD20 pKa = 3.91LVNAAFHH27 pKa = 6.22AAEE30 pKa = 4.8LSQDD34 pKa = 3.92DD35 pKa = 4.14FCEE38 pKa = 4.34LTRR41 pKa = 11.84SMDD44 pKa = 3.72AADD47 pKa = 3.78EE48 pKa = 3.95QLGEE52 pKa = 4.08GSEE55 pKa = 4.37GKK57 pKa = 10.79VMAGVLRR64 pKa = 11.84GQPVAVKK71 pKa = 10.21IMHH74 pKa = 5.56QTLPDD79 pKa = 3.55LSMEE83 pKa = 4.46SLASMSAYY91 pKa = 10.6SDD93 pKa = 3.08VTMAVLAAMCDD104 pKa = 3.67EE105 pKa = 4.31VLKK108 pKa = 11.04LMPCCPIAFAIEE120 pKa = 4.05VGAVEE125 pKa = 5.22AIGGEE130 pKa = 4.15PSCIDD135 pKa = 3.45VLIVSVMPLALGSLEE150 pKa = 4.55DD151 pKa = 4.04LQLKK155 pKa = 10.5LEE157 pKa = 4.8GYY159 pKa = 9.95SYY161 pKa = 11.0LPSIPQVDD169 pKa = 3.13ICIQQ173 pKa = 3.14

Molecular weight: 18.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A699YNW0|A0A699YNW0_HAELA Uncharacterized protein (Fragment) OS=Haematococcus lacustris OX=44745 GN=HaLaN_03581 PE=4 SV=1
PP1 pKa = 6.74GQASGSRR8 pKa = 11.84HH9 pKa = 3.74LTQRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84QWGRR19 pKa = 11.84WQQRR23 pKa = 11.84ASPSASWAPSPAAAVRR39 pKa = 11.84GSVSASWRR47 pKa = 11.84MTSVRR52 pKa = 11.84WRR54 pKa = 11.84RR55 pKa = 11.84APSPTRR61 pKa = 11.84WRR63 pKa = 11.84RR64 pKa = 11.84WLPP67 pKa = 3.38

Molecular weight: 7.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28121

0

28121

5197905

8

2951

184.8

19.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.24 ± 0.028

2.16 ± 0.01

4.363 ± 0.013

5.024 ± 0.022

2.555 ± 0.011

7.855 ± 0.024

2.74 ± 0.011

2.871 ± 0.013

3.747 ± 0.019

10.336 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.008

2.441 ± 0.01

6.325 ± 0.022

5.437 ± 0.019

6.105 ± 0.014

7.455 ± 0.019

5.269 ± 0.013

6.87 ± 0.016

1.589 ± 0.008

1.978 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski