narna-like virus 6
Average proteome isoelectric point is 7.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIM6|A0A1L3KIM6_9VIRU RNA-dependent RNA polymerase OS=narna-like virus 6 OX=1923780 PE=4 SV=1
MM1 pKa = 7.38 LNVYY5 pKa = 10.03 HH6 pKa = 6.6 FVYY9 pKa = 10.03 RR10 pKa = 11.84 QYY12 pKa = 11.38 GFHH15 pKa = 6.67 FRR17 pKa = 11.84 PSRR20 pKa = 11.84 EE21 pKa = 3.91 DD22 pKa = 3.66 LIFWEE27 pKa = 4.39 WLGATNRR34 pKa = 11.84 LEE36 pKa = 4.91 TYY38 pKa = 10.25 LKK40 pKa = 10.13 WKK42 pKa = 7.39 TANMLSRR49 pKa = 11.84 ALGSSEE55 pKa = 5.65 LVPCPLDD62 pKa = 3.72 LSHH65 pKa = 7.25 IPPHH69 pKa = 5.92 FDD71 pKa = 3.3 YY72 pKa = 11.49 YY73 pKa = 11.03 FGADD77 pKa = 3.14 RR78 pKa = 11.84 LGLLRR83 pKa = 11.84 TDD85 pKa = 3.61 QGFHH89 pKa = 6.65 RR90 pKa = 11.84 MKK92 pKa = 10.62 LHH94 pKa = 6.64 RR95 pKa = 11.84 KK96 pKa = 7.61 STDD99 pKa = 2.9 RR100 pKa = 11.84 YY101 pKa = 10.59 ASFCFSLYY109 pKa = 9.35 QSKK112 pKa = 10.18 AASLPAEE119 pKa = 4.2 EE120 pKa = 3.94 WRR122 pKa = 11.84 VRR124 pKa = 11.84 SEE126 pKa = 3.62 VVAAMRR132 pKa = 11.84 RR133 pKa = 11.84 LTEE136 pKa = 4.24 KK137 pKa = 10.39 PDD139 pKa = 3.74 PLVPKK144 pKa = 10.15 ILTNGARR151 pKa = 11.84 LDD153 pKa = 3.76 YY154 pKa = 11.0 DD155 pKa = 3.89 LLEE158 pKa = 4.54 EE159 pKa = 4.32 AVHH162 pKa = 5.71 RR163 pKa = 11.84 TIEE166 pKa = 4.42 LFSPCNRR173 pKa = 11.84 GDD175 pKa = 3.26 RR176 pKa = 11.84 LARR179 pKa = 11.84 RR180 pKa = 11.84 RR181 pKa = 11.84 LPSRR185 pKa = 11.84 GAVLGSSRR193 pKa = 11.84 SEE195 pKa = 3.93 GGALGQIARR204 pKa = 11.84 LGHH207 pKa = 5.88 GCDD210 pKa = 3.48 FFVPDD215 pKa = 3.98 RR216 pKa = 11.84 YY217 pKa = 10.86 LIGFYY222 pKa = 10.0 EE223 pKa = 5.24 GPAGAVEE230 pKa = 4.38 VRR232 pKa = 11.84 SSCEE236 pKa = 3.86 PEE238 pKa = 4.03 DD239 pKa = 3.77 VAWAYY244 pKa = 11.04 DD245 pKa = 4.18 FFGHH249 pKa = 6.82 WGMSHH254 pKa = 7.56 DD255 pKa = 4.22 VPCWPVGLPEE265 pKa = 4.15 PFKK268 pKa = 11.31 VRR270 pKa = 11.84 VITKK274 pKa = 9.96 GYY276 pKa = 9.3 APSYY280 pKa = 8.19 NHH282 pKa = 6.4 ARR284 pKa = 11.84 AYY286 pKa = 9.87 QPRR289 pKa = 11.84 LWRR292 pKa = 11.84 SLQEE296 pKa = 4.01 HH297 pKa = 5.59 NVFRR301 pKa = 11.84 LTGEE305 pKa = 4.19 MVTGSAIQSFLSEE318 pKa = 4.62 CPVDD322 pKa = 3.71 SGAFFLSGDD331 pKa = 3.75 YY332 pKa = 10.87 KK333 pKa = 10.82 EE334 pKa = 4.85 ATDD337 pKa = 5.24 HH338 pKa = 6.59 IPSEE342 pKa = 4.4 IANLMCMRR350 pKa = 11.84 FCDD353 pKa = 3.48 RR354 pKa = 11.84 LGIPLEE360 pKa = 4.15 VVGRR364 pKa = 11.84 IARR367 pKa = 11.84 ALTGHH372 pKa = 7.43 ILPTPEE378 pKa = 5.12 LVDD381 pKa = 3.37 PTMGLGSQGCMVRR394 pKa = 11.84 RR395 pKa = 11.84 QKK397 pKa = 10.57 KK398 pKa = 7.7 GQLMGSPISFPFLCLYY414 pKa = 10.9 NLVLQILFHH423 pKa = 6.53 FVAEE427 pKa = 4.64 GEE429 pKa = 4.35 WIRR432 pKa = 11.84 DD433 pKa = 3.36 LSGVRR438 pKa = 11.84 NLINGDD444 pKa = 3.89 DD445 pKa = 3.8 LLSYY449 pKa = 10.51 LRR451 pKa = 11.84 EE452 pKa = 4.0 RR453 pKa = 11.84 GLYY456 pKa = 9.65 RR457 pKa = 11.84 IWEE460 pKa = 4.28 EE461 pKa = 3.87 VVEE464 pKa = 4.26 WGGLKK469 pKa = 10.39 PSVGKK474 pKa = 8.85 TIVSQRR480 pKa = 11.84 YY481 pKa = 6.82 LTINSKK487 pKa = 10.14 LFRR490 pKa = 11.84 VDD492 pKa = 3.38 SAVDD496 pKa = 3.25 QHH498 pKa = 5.83 GTPYY502 pKa = 9.36 WRR504 pKa = 11.84 NRR506 pKa = 11.84 HH507 pKa = 5.62 LPHH510 pKa = 6.3 VQLQLAIGSMKK521 pKa = 10.28 SGHH524 pKa = 6.48 IDD526 pKa = 3.07 EE527 pKa = 5.55 DD528 pKa = 3.92 SWVLSSTSPRR538 pKa = 11.84 SRR540 pKa = 11.84 SRR542 pKa = 11.84 MWQEE546 pKa = 4.41 FLDD549 pKa = 4.06 SCPDD553 pKa = 3.45 KK554 pKa = 11.32 DD555 pKa = 4.09 RR556 pKa = 11.84 AWSFLFSANRR566 pKa = 11.84 GLLRR570 pKa = 11.84 GLMDD574 pKa = 4.94 KK575 pKa = 11.18 YY576 pKa = 9.57 PTCTLCLPTEE586 pKa = 4.3 AGGLGFPLPPIDD598 pKa = 4.2 SRR600 pKa = 11.84 WYY602 pKa = 7.72 VQRR605 pKa = 11.84 APRR608 pKa = 11.84 SVDD611 pKa = 2.82 RR612 pKa = 11.84 MKK614 pKa = 11.24 ARR616 pKa = 11.84 LLLSAGLPSHH626 pKa = 6.96 DD627 pKa = 3.72 QLRR630 pKa = 11.84 SRR632 pKa = 11.84 WLSSLASEE640 pKa = 5.43 DD641 pKa = 3.84 PRR643 pKa = 11.84 TADD646 pKa = 3.37 ALLFGDD652 pKa = 4.4 LLRR655 pKa = 11.84 YY656 pKa = 9.03 QKK658 pKa = 9.69 STGCPLVLEE667 pKa = 4.54 TLDD670 pKa = 3.58 EE671 pKa = 4.27 QVGKK675 pKa = 10.61 EE676 pKa = 4.07 KK677 pKa = 10.77 DD678 pKa = 3.82 LPPPILACPLAGGVIDD694 pKa = 4.39 PLEE697 pKa = 4.07 EE698 pKa = 4.38 HH699 pKa = 6.94 LLLASQRR706 pKa = 11.84 RR707 pKa = 11.84 VIRR710 pKa = 11.84 DD711 pKa = 2.67 IDD713 pKa = 3.65 RR714 pKa = 11.84 AVRR717 pKa = 11.84 AYY719 pKa = 10.35 SRR721 pKa = 11.84 SGRR724 pKa = 11.84 GPWTQCEE731 pKa = 3.75 ISDD734 pKa = 3.66 FLRR737 pKa = 11.84 RR738 pKa = 11.84 FAWRR742 pKa = 11.84 RR743 pKa = 11.84 KK744 pKa = 7.47 YY745 pKa = 10.74 GRR747 pKa = 11.84 YY748 pKa = 9.5 LDD750 pKa = 3.93 RR751 pKa = 11.84 TCGFF755 pKa = 3.9
Molecular weight: 86.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.829
IPC2_protein 8.024
IPC_protein 7.834
Toseland 7.454
ProMoST 8.404
Dawson 8.536
Bjellqvist 8.916
Wikipedia 8.478
Rodwell 8.565
Grimsley 7.497
Solomon 8.595
Lehninger 8.595
Nozaki 9.063
DTASelect 8.624
Thurlkill 8.668
EMBOSS 8.726
Sillero 8.99
Patrickios 4.418
IPC_peptide 8.58
IPC2_peptide 8.068
IPC2.peptide.svr19 8.138
Protein with the highest isoelectric point:
>tr|A0A1L3KIM6|A0A1L3KIM6_9VIRU RNA-dependent RNA polymerase OS=narna-like virus 6 OX=1923780 PE=4 SV=1
MM1 pKa = 7.38 LNVYY5 pKa = 10.03 HH6 pKa = 6.6 FVYY9 pKa = 10.03 RR10 pKa = 11.84 QYY12 pKa = 11.38 GFHH15 pKa = 6.67 FRR17 pKa = 11.84 PSRR20 pKa = 11.84 EE21 pKa = 3.91 DD22 pKa = 3.66 LIFWEE27 pKa = 4.39 WLGATNRR34 pKa = 11.84 LEE36 pKa = 4.91 TYY38 pKa = 10.25 LKK40 pKa = 10.13 WKK42 pKa = 7.39 TANMLSRR49 pKa = 11.84 ALGSSEE55 pKa = 5.65 LVPCPLDD62 pKa = 3.72 LSHH65 pKa = 7.25 IPPHH69 pKa = 5.92 FDD71 pKa = 3.3 YY72 pKa = 11.49 YY73 pKa = 11.03 FGADD77 pKa = 3.14 RR78 pKa = 11.84 LGLLRR83 pKa = 11.84 TDD85 pKa = 3.61 QGFHH89 pKa = 6.65 RR90 pKa = 11.84 MKK92 pKa = 10.62 LHH94 pKa = 6.64 RR95 pKa = 11.84 KK96 pKa = 7.61 STDD99 pKa = 2.9 RR100 pKa = 11.84 YY101 pKa = 10.59 ASFCFSLYY109 pKa = 9.35 QSKK112 pKa = 10.18 AASLPAEE119 pKa = 4.2 EE120 pKa = 3.94 WRR122 pKa = 11.84 VRR124 pKa = 11.84 SEE126 pKa = 3.62 VVAAMRR132 pKa = 11.84 RR133 pKa = 11.84 LTEE136 pKa = 4.24 KK137 pKa = 10.39 PDD139 pKa = 3.74 PLVPKK144 pKa = 10.15 ILTNGARR151 pKa = 11.84 LDD153 pKa = 3.76 YY154 pKa = 11.0 DD155 pKa = 3.89 LLEE158 pKa = 4.54 EE159 pKa = 4.32 AVHH162 pKa = 5.71 RR163 pKa = 11.84 TIEE166 pKa = 4.42 LFSPCNRR173 pKa = 11.84 GDD175 pKa = 3.26 RR176 pKa = 11.84 LARR179 pKa = 11.84 RR180 pKa = 11.84 RR181 pKa = 11.84 LPSRR185 pKa = 11.84 GAVLGSSRR193 pKa = 11.84 SEE195 pKa = 3.93 GGALGQIARR204 pKa = 11.84 LGHH207 pKa = 5.88 GCDD210 pKa = 3.48 FFVPDD215 pKa = 3.98 RR216 pKa = 11.84 YY217 pKa = 10.86 LIGFYY222 pKa = 10.0 EE223 pKa = 5.24 GPAGAVEE230 pKa = 4.38 VRR232 pKa = 11.84 SSCEE236 pKa = 3.86 PEE238 pKa = 4.03 DD239 pKa = 3.77 VAWAYY244 pKa = 11.04 DD245 pKa = 4.18 FFGHH249 pKa = 6.82 WGMSHH254 pKa = 7.56 DD255 pKa = 4.22 VPCWPVGLPEE265 pKa = 4.15 PFKK268 pKa = 11.31 VRR270 pKa = 11.84 VITKK274 pKa = 9.96 GYY276 pKa = 9.3 APSYY280 pKa = 8.19 NHH282 pKa = 6.4 ARR284 pKa = 11.84 AYY286 pKa = 9.87 QPRR289 pKa = 11.84 LWRR292 pKa = 11.84 SLQEE296 pKa = 4.01 HH297 pKa = 5.59 NVFRR301 pKa = 11.84 LTGEE305 pKa = 4.19 MVTGSAIQSFLSEE318 pKa = 4.62 CPVDD322 pKa = 3.71 SGAFFLSGDD331 pKa = 3.75 YY332 pKa = 10.87 KK333 pKa = 10.82 EE334 pKa = 4.85 ATDD337 pKa = 5.24 HH338 pKa = 6.59 IPSEE342 pKa = 4.4 IANLMCMRR350 pKa = 11.84 FCDD353 pKa = 3.48 RR354 pKa = 11.84 LGIPLEE360 pKa = 4.15 VVGRR364 pKa = 11.84 IARR367 pKa = 11.84 ALTGHH372 pKa = 7.43 ILPTPEE378 pKa = 5.12 LVDD381 pKa = 3.37 PTMGLGSQGCMVRR394 pKa = 11.84 RR395 pKa = 11.84 QKK397 pKa = 10.57 KK398 pKa = 7.7 GQLMGSPISFPFLCLYY414 pKa = 10.9 NLVLQILFHH423 pKa = 6.53 FVAEE427 pKa = 4.64 GEE429 pKa = 4.35 WIRR432 pKa = 11.84 DD433 pKa = 3.36 LSGVRR438 pKa = 11.84 NLINGDD444 pKa = 3.89 DD445 pKa = 3.8 LLSYY449 pKa = 10.51 LRR451 pKa = 11.84 EE452 pKa = 4.0 RR453 pKa = 11.84 GLYY456 pKa = 9.65 RR457 pKa = 11.84 IWEE460 pKa = 4.28 EE461 pKa = 3.87 VVEE464 pKa = 4.26 WGGLKK469 pKa = 10.39 PSVGKK474 pKa = 8.85 TIVSQRR480 pKa = 11.84 YY481 pKa = 6.82 LTINSKK487 pKa = 10.14 LFRR490 pKa = 11.84 VDD492 pKa = 3.38 SAVDD496 pKa = 3.25 QHH498 pKa = 5.83 GTPYY502 pKa = 9.36 WRR504 pKa = 11.84 NRR506 pKa = 11.84 HH507 pKa = 5.62 LPHH510 pKa = 6.3 VQLQLAIGSMKK521 pKa = 10.28 SGHH524 pKa = 6.48 IDD526 pKa = 3.07 EE527 pKa = 5.55 DD528 pKa = 3.92 SWVLSSTSPRR538 pKa = 11.84 SRR540 pKa = 11.84 SRR542 pKa = 11.84 MWQEE546 pKa = 4.41 FLDD549 pKa = 4.06 SCPDD553 pKa = 3.45 KK554 pKa = 11.32 DD555 pKa = 4.09 RR556 pKa = 11.84 AWSFLFSANRR566 pKa = 11.84 GLLRR570 pKa = 11.84 GLMDD574 pKa = 4.94 KK575 pKa = 11.18 YY576 pKa = 9.57 PTCTLCLPTEE586 pKa = 4.3 AGGLGFPLPPIDD598 pKa = 4.2 SRR600 pKa = 11.84 WYY602 pKa = 7.72 VQRR605 pKa = 11.84 APRR608 pKa = 11.84 SVDD611 pKa = 2.82 RR612 pKa = 11.84 MKK614 pKa = 11.24 ARR616 pKa = 11.84 LLLSAGLPSHH626 pKa = 6.96 DD627 pKa = 3.72 QLRR630 pKa = 11.84 SRR632 pKa = 11.84 WLSSLASEE640 pKa = 5.43 DD641 pKa = 3.84 PRR643 pKa = 11.84 TADD646 pKa = 3.37 ALLFGDD652 pKa = 4.4 LLRR655 pKa = 11.84 YY656 pKa = 9.03 QKK658 pKa = 9.69 STGCPLVLEE667 pKa = 4.54 TLDD670 pKa = 3.58 EE671 pKa = 4.27 QVGKK675 pKa = 10.61 EE676 pKa = 4.07 KK677 pKa = 10.77 DD678 pKa = 3.82 LPPPILACPLAGGVIDD694 pKa = 4.39 PLEE697 pKa = 4.07 EE698 pKa = 4.38 HH699 pKa = 6.94 LLLASQRR706 pKa = 11.84 RR707 pKa = 11.84 VIRR710 pKa = 11.84 DD711 pKa = 2.67 IDD713 pKa = 3.65 RR714 pKa = 11.84 AVRR717 pKa = 11.84 AYY719 pKa = 10.35 SRR721 pKa = 11.84 SGRR724 pKa = 11.84 GPWTQCEE731 pKa = 3.75 ISDD734 pKa = 3.66 FLRR737 pKa = 11.84 RR738 pKa = 11.84 FAWRR742 pKa = 11.84 RR743 pKa = 11.84 KK744 pKa = 7.47 YY745 pKa = 10.74 GRR747 pKa = 11.84 YY748 pKa = 9.5 LDD750 pKa = 3.93 RR751 pKa = 11.84 TCGFF755 pKa = 3.9
Molecular weight: 86.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.829
IPC2_protein 8.024
IPC_protein 7.834
Toseland 7.454
ProMoST 8.404
Dawson 8.536
Bjellqvist 8.916
Wikipedia 8.478
Rodwell 8.565
Grimsley 7.497
Solomon 8.595
Lehninger 8.595
Nozaki 9.063
DTASelect 8.624
Thurlkill 8.668
EMBOSS 8.726
Sillero 8.99
Patrickios 4.418
IPC_peptide 8.58
IPC2_peptide 8.068
IPC2.peptide.svr19 8.138
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
755
755
755
755.0
86.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.225 ± 0.0
2.384 ± 0.0
5.828 ± 0.0
5.298 ± 0.0
4.503 ± 0.0
7.815 ± 0.0
2.781 ± 0.0
3.709 ± 0.0
3.046 ± 0.0
12.318 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.987 ± 0.0
1.854 ± 0.0
6.358 ± 0.0
2.914 ± 0.0
9.801 ± 0.0
7.947 ± 0.0
3.576 ± 0.0
5.563 ± 0.0
2.517 ± 0.0
3.576 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here