Fictibacillus aquaticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Fictibacillus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3939 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A235F8P6|A0A235F8P6_9BACI Autolysin OS=Fictibacillus aquaticus OX=2021314 GN=CGZ90_13225 PE=4 SV=1
MM1 pKa = 7.5TNTKK5 pKa = 10.39KK6 pKa = 10.13MLGGLLSAAVMLSGCSTSYY25 pKa = 10.39TEE27 pKa = 5.53SYY29 pKa = 11.01DD30 pKa = 3.51EE31 pKa = 4.68EE32 pKa = 5.72IYY34 pKa = 11.03DD35 pKa = 3.65EE36 pKa = 4.35EE37 pKa = 5.77VYY39 pKa = 10.36TEE41 pKa = 4.22PDD43 pKa = 3.17FEE45 pKa = 5.1NDD47 pKa = 3.23YY48 pKa = 11.61SSDD51 pKa = 3.02TSYY54 pKa = 11.5YY55 pKa = 9.84PEE57 pKa = 5.25DD58 pKa = 4.04DD59 pKa = 3.44EE60 pKa = 6.17GYY62 pKa = 11.16GEE64 pKa = 4.83IEE66 pKa = 4.23LDD68 pKa = 3.72HH69 pKa = 6.43LQSYY73 pKa = 9.83YY74 pKa = 10.29PDD76 pKa = 4.65LDD78 pKa = 3.83LEE80 pKa = 4.29AMEE83 pKa = 6.45DD84 pKa = 3.77YY85 pKa = 11.18LEE87 pKa = 4.5EE88 pKa = 4.94NGLEE92 pKa = 4.11YY93 pKa = 11.13NEE95 pKa = 4.66EE96 pKa = 3.86EE97 pKa = 5.11LIAFLDD103 pKa = 3.9DD104 pKa = 3.69LQEE107 pKa = 4.34NGEE110 pKa = 4.28TTVYY114 pKa = 10.98DD115 pKa = 3.65NYY117 pKa = 11.29YY118 pKa = 10.39ADD120 pKa = 4.27EE121 pKa = 4.38NAFPWWIFLLANTTKK136 pKa = 10.06TKK138 pKa = 10.02PSSKK142 pKa = 10.19IKK144 pKa = 9.05FTKK147 pKa = 9.04PTTKK151 pKa = 9.24PTTTTTTKK159 pKa = 9.7STTKK163 pKa = 9.96KK164 pKa = 10.41SSGFGSGSTSSGSS177 pKa = 3.18

Molecular weight: 19.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A235F8V5|A0A235F8V5_9BACI Short-chain fatty acid transporter OS=Fictibacillus aquaticus OX=2021314 GN=CGZ90_13500 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.74PTFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.45GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.82VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3939

0

3939

1115026

25

3486

283.1

31.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.747 ± 0.041

0.719 ± 0.012

5.038 ± 0.035

7.424 ± 0.052

4.527 ± 0.037

7.141 ± 0.04

2.093 ± 0.02

7.086 ± 0.041

6.98 ± 0.047

9.718 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.896 ± 0.025

4.082 ± 0.028

3.676 ± 0.024

3.514 ± 0.025

4.104 ± 0.028

6.383 ± 0.036

5.294 ± 0.037

7.033 ± 0.033

1.067 ± 0.015

3.478 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski