Vibrio phage CKB-S1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E1GE49|A0A1E1GE49_9CAUD Uncharacterized protein OS=Vibrio phage CKB-S1 OX=1903259 PE=4 SV=1
MM1 pKa = 7.73LIKK4 pKa = 10.0LTRR7 pKa = 11.84ATRR10 pKa = 11.84YY11 pKa = 9.82LGAVLNIGGAGGAGFVYY28 pKa = 10.06PDD30 pKa = 3.19SADD33 pKa = 3.64RR34 pKa = 11.84PFATIAARR42 pKa = 11.84NTWAAANLGDD52 pKa = 4.61LVDD55 pKa = 4.38GSTVVQVAGTPATWYY70 pKa = 9.68LWTGEE75 pKa = 4.29TSPSSHH81 pKa = 7.66DD82 pKa = 3.39SALWIDD88 pKa = 4.17ATPLIQGPAGEE99 pKa = 4.92DD100 pKa = 4.11GIDD103 pKa = 3.48GTALEE108 pKa = 4.63FTSLSARR115 pKa = 11.84DD116 pKa = 3.49SFFISRR122 pKa = 11.84PDD124 pKa = 3.34LLLNGLPVLVNIGNNTVGIYY144 pKa = 8.68TWTGPTNPSDD154 pKa = 4.22LVASDD159 pKa = 4.04TRR161 pKa = 11.84WSQASQAVPNNSLFVGSTRR180 pKa = 11.84VSNAVEE186 pKa = 4.15NIVAEE191 pKa = 4.37TADD194 pKa = 3.39GRR196 pKa = 11.84QFLATGVRR204 pKa = 11.84FDD206 pKa = 4.38DD207 pKa = 3.76TGSRR211 pKa = 11.84PFGFTEE217 pKa = 4.27LAAATTLNVNTANTQDD233 pKa = 3.58MPDD236 pKa = 4.11PFTVQYY242 pKa = 7.66TTFGDD247 pKa = 3.63NLTTDD252 pKa = 4.27FDD254 pKa = 5.28FIPAEE259 pKa = 4.15AGEE262 pKa = 3.99LRR264 pKa = 11.84AEE266 pKa = 4.37FFLGADD272 pKa = 3.85DD273 pKa = 3.9QAPLIFDD280 pKa = 3.32EE281 pKa = 4.9RR282 pKa = 11.84RR283 pKa = 11.84AVTQAEE289 pKa = 3.99VDD291 pKa = 3.15AGEE294 pKa = 4.24FVSFGVGNPYY304 pKa = 10.82LLDD307 pKa = 3.93EE308 pKa = 4.66GLQLFARR315 pKa = 11.84FSGVQLRR322 pKa = 11.84GGVVDD327 pKa = 4.9NPGTPLDD334 pKa = 3.95GQTIISFRR342 pKa = 11.84STVQAFTARR351 pKa = 11.84QVMRR355 pKa = 11.84ADD357 pKa = 3.82EE358 pKa = 4.27FTGAAVRR365 pKa = 11.84DD366 pKa = 4.21LLTALTGGDD375 pKa = 4.13RR376 pKa = 11.84LPASAIRR383 pKa = 11.84DD384 pKa = 3.66LPAGVTLRR392 pKa = 11.84TASEE396 pKa = 4.04TADD399 pKa = 3.82LLEE402 pKa = 4.67TLTGDD407 pKa = 4.17DD408 pKa = 4.13RR409 pKa = 11.84LAASAIRR416 pKa = 11.84DD417 pKa = 3.67LPADD421 pKa = 3.11VDD423 pKa = 3.91NFVDD427 pKa = 3.57QLTVGLSGQDD437 pKa = 3.22LTITLGRR444 pKa = 11.84TGTLPDD450 pKa = 4.05LSQTVTLPSGGGTDD464 pKa = 4.17PGPGPDD470 pKa = 2.96VLYY473 pKa = 10.72YY474 pKa = 10.84GRR476 pKa = 11.84STSNNPATVDD486 pKa = 3.98LATLTQIDD494 pKa = 4.07PTDD497 pKa = 4.05PQTVSTGVAVAGDD510 pKa = 3.56YY511 pKa = 10.75FIILTANTHH520 pKa = 7.17DD521 pKa = 3.43ITTITDD527 pKa = 3.33TVLQQDD533 pKa = 3.92VTSLFTKK540 pKa = 10.28TEE542 pKa = 3.96DD543 pKa = 3.34VRR545 pKa = 11.84AEE547 pKa = 4.06GAVTLDD553 pKa = 3.76SYY555 pKa = 11.96VIGPLNAGVNEE566 pKa = 4.09QYY568 pKa = 11.28VLEE571 pKa = 4.45FF572 pKa = 4.38

Molecular weight: 60.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E1GEL5|A0A1E1GEL5_9CAUD Gp62 OS=Vibrio phage CKB-S1 OX=1903259 GN=BcepB1A gene62 PE=4 SV=1
MM1 pKa = 7.64ASCPHH6 pKa = 4.22THH8 pKa = 6.29FRR10 pKa = 11.84RR11 pKa = 11.84GTTVLVILKK20 pKa = 10.23DD21 pKa = 3.37STKK24 pKa = 10.15RR25 pKa = 11.84VCKK28 pKa = 10.34FKK30 pKa = 10.69EE31 pKa = 4.12SRR33 pKa = 11.84SKK35 pKa = 10.9HH36 pKa = 4.5IVLWDD41 pKa = 3.7GEE43 pKa = 4.43KK44 pKa = 10.69LPHH47 pKa = 6.45KK48 pKa = 10.25LIRR51 pKa = 11.84QVLIYY56 pKa = 9.91RR57 pKa = 11.84RR58 pKa = 11.84KK59 pKa = 10.05GGQAA63 pKa = 2.98

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

15186

36

1064

261.8

28.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.641 ± 0.417

0.711 ± 0.154

6.249 ± 0.222

5.9 ± 0.269

3.464 ± 0.181

8.205 ± 0.188

1.791 ± 0.191

4.682 ± 0.22

3.674 ± 0.357

8.165 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.496 ± 0.207

4.32 ± 0.213

4.748 ± 0.221

5.123 ± 0.511

6.091 ± 0.275

4.899 ± 0.174

6.934 ± 0.392

7.698 ± 0.258

1.468 ± 0.159

2.739 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski