Rhodococcus phage Peregrin

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Weaselvirus; unclassified Weaselvirus

Average proteome isoelectric point is 5.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UKS2|A0A2U8UKS2_9CAUD Uncharacterized protein OS=Rhodococcus phage Peregrin OX=2182361 GN=201 PE=4 SV=1
MM1 pKa = 7.42TKK3 pKa = 9.38LTIKK7 pKa = 10.64SEE9 pKa = 4.11DD10 pKa = 3.97LIAEE14 pKa = 4.42VIKK17 pKa = 10.5IAQANPNYY25 pKa = 10.48VYY27 pKa = 11.1DD28 pKa = 4.02NVGGCHH34 pKa = 5.78YY35 pKa = 10.44SSEE38 pKa = 4.3STAGGSCLFGLALFNLGVDD57 pKa = 4.67FFTLRR62 pKa = 11.84ALDD65 pKa = 3.58SFEE68 pKa = 6.15DD69 pKa = 4.04YY70 pKa = 7.77EE71 pKa = 4.5TTIGCLADD79 pKa = 4.0PDD81 pKa = 4.2STIEE85 pKa = 4.11FPVDD89 pKa = 3.54IIGTNEE95 pKa = 3.48QAAAMADD102 pKa = 3.53AQQSQDD108 pKa = 2.94IQKK111 pKa = 9.28PWGEE115 pKa = 4.16AIIPLLDD122 pKa = 4.4LNSSTDD128 pKa = 2.97

Molecular weight: 13.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UKP8|A0A2U8UKP8_9CAUD Thioredoxin OS=Rhodococcus phage Peregrin OX=2182361 GN=171 PE=4 SV=1
MM1 pKa = 7.26MNYY4 pKa = 10.31GSGIVNRR11 pKa = 11.84HH12 pKa = 5.77RR13 pKa = 11.84NDD15 pKa = 3.48SFDD18 pKa = 3.54LTTMKK23 pKa = 10.68GIRR26 pKa = 11.84AAADD30 pKa = 3.36RR31 pKa = 11.84VEE33 pKa = 4.56KK34 pKa = 10.84VPSHH38 pKa = 6.81ILLQRR43 pKa = 11.84YY44 pKa = 8.38NSYY47 pKa = 10.13RR48 pKa = 11.84RR49 pKa = 11.84YY50 pKa = 9.92MIFKK54 pKa = 10.31RR55 pKa = 11.84LWKK58 pKa = 10.47KK59 pKa = 10.26LFKK62 pKa = 10.82

Molecular weight: 7.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

267

0

267

39698

29

2464

148.7

16.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.149 ± 0.298

1.189 ± 0.113

6.978 ± 0.109

7.837 ± 0.246

3.789 ± 0.143

6.577 ± 0.221

2.025 ± 0.104

6.514 ± 0.154

6.61 ± 0.163

7.832 ± 0.149

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.632 ± 0.095

4.837 ± 0.123

3.668 ± 0.103

3.232 ± 0.116

4.64 ± 0.133

6.182 ± 0.177

5.741 ± 0.233

6.587 ± 0.146

1.864 ± 0.096

4.119 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski