Intestinibaculum porci
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2890 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G9JBQ8|A0A3G9JBQ8_9FIRM L-lactate dehydrogenase OS=Intestinibaculum porci OX=2487118 GN=ldh3 PE=3 SV=1
MM1 pKa = 7.59 AMKK4 pKa = 10.09 DD5 pKa = 3.74 YY6 pKa = 10.52 EE7 pKa = 4.29 ISSDD11 pKa = 3.64 PLTEE15 pKa = 5.28 KK16 pKa = 10.99 DD17 pKa = 3.85 DD18 pKa = 5.5 DD19 pKa = 4.81 YY20 pKa = 11.74 DD21 pKa = 4.66 PEE23 pKa = 4.28 MYY25 pKa = 10.54 EE26 pKa = 4.56 DD27 pKa = 4.55 GSTEE31 pKa = 4.24 AMGAYY36 pKa = 7.63 YY37 pKa = 9.82 TYY39 pKa = 10.58 IANSLSRR46 pKa = 11.84 IKK48 pKa = 9.69 TALSATSSSKK58 pKa = 10.55 KK59 pKa = 9.67 IKK61 pKa = 10.33 EE62 pKa = 4.06 LMSSDD67 pKa = 3.65 EE68 pKa = 4.72 NINIYY73 pKa = 10.77 QMLIDD78 pKa = 5.32 DD79 pKa = 4.72 PRR81 pKa = 11.84 LMKK84 pKa = 10.31 DD85 pKa = 2.56 IRR87 pKa = 11.84 EE88 pKa = 4.29 AMSSDD93 pKa = 3.08 IIKK96 pKa = 10.58 PFDD99 pKa = 3.91 EE100 pKa = 5.55 DD101 pKa = 4.14 NLLEE105 pKa = 4.68 LYY107 pKa = 9.25 MNKK110 pKa = 9.3 VSDD113 pKa = 5.11 LISQIEE119 pKa = 4.05 YY120 pKa = 10.07 VYY122 pKa = 10.75 PEE124 pKa = 3.52 IYY126 pKa = 6.96 MTLMHH131 pKa = 7.28 KK132 pKa = 10.21 ILPYY136 pKa = 10.54 SVASYY141 pKa = 9.26 YY142 pKa = 11.07 DD143 pKa = 3.29 QMVSEE148 pKa = 4.31 EE149 pKa = 3.88 NDD151 pKa = 3.0 RR152 pKa = 11.84 DD153 pKa = 3.7 FEE155 pKa = 4.58 YY156 pKa = 10.51 AIQDD160 pKa = 3.9 DD161 pKa = 4.37 DD162 pKa = 4.11 VQFF165 pKa = 3.99
Molecular weight: 19.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.768
IPC2_protein 3.948
IPC_protein 3.935
Toseland 3.732
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.846
Rodwell 3.757
Grimsley 3.63
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.253
Thurlkill 3.77
EMBOSS 3.859
Sillero 4.05
Patrickios 1.914
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.949
Protein with the highest isoelectric point:
>tr|A0A3G9JAT8|A0A3G9JAT8_9FIRM PTS EIIB type-3 domain-containing protein OS=Intestinibaculum porci OX=2487118 GN=SG0102_26300 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.62 RR10 pKa = 11.84 KK11 pKa = 9.56 RR12 pKa = 11.84 AKK14 pKa = 8.76 THH16 pKa = 5.23 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.34 VIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 8.84 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.41 VLSVV44 pKa = 3.22
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2890
0
2890
889665
40
4468
307.8
34.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.096 ± 0.041
1.239 ± 0.016
6.225 ± 0.043
6.255 ± 0.051
4.215 ± 0.035
6.07 ± 0.041
2.662 ± 0.027
7.511 ± 0.048
7.861 ± 0.049
9.166 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.05 ± 0.027
4.717 ± 0.039
3.094 ± 0.022
3.281 ± 0.027
3.943 ± 0.035
5.907 ± 0.043
5.683 ± 0.053
6.488 ± 0.037
0.688 ± 0.013
4.85 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here