Amino acid dipepetide frequency for Bacillus phage BC-T25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.441AlaAla: 5.441 ± 0.497
0.401AlaCys: 0.401 ± 0.1
3.627AlaAsp: 3.627 ± 0.297
5.336AlaGlu: 5.336 ± 0.391
2.425AlaPhe: 2.425 ± 0.2
4.049AlaGly: 4.049 ± 0.433
1.118AlaHis: 1.118 ± 0.145
4.408AlaIle: 4.408 ± 0.354
5.082AlaLys: 5.082 ± 0.33
5.778AlaLeu: 5.778 ± 0.353
2.257AlaMet: 2.257 ± 0.218
3.206AlaAsn: 3.206 ± 0.348
2.678AlaPro: 2.678 ± 0.347
2.995AlaGln: 2.995 ± 0.279
2.657AlaArg: 2.657 ± 0.257
3.311AlaSer: 3.311 ± 0.318
4.323AlaThr: 4.323 ± 0.457
3.775AlaVal: 3.775 ± 0.299
1.012AlaTrp: 1.012 ± 0.134
2.51AlaTyr: 2.51 ± 0.2
0.0AlaXaa: 0.0 ± 0.0
Cys
0.232CysAla: 0.232 ± 0.06
0.105CysCys: 0.105 ± 0.044
0.38CysAsp: 0.38 ± 0.093
0.506CysGlu: 0.506 ± 0.108
0.38CysPhe: 0.38 ± 0.087
0.612CysGly: 0.612 ± 0.12
0.19CysHis: 0.19 ± 0.074
0.401CysIle: 0.401 ± 0.075
0.822CysLys: 0.822 ± 0.154
0.401CysLeu: 0.401 ± 0.086
0.253CysMet: 0.253 ± 0.083
0.654CysAsn: 0.654 ± 0.159
0.337CysPro: 0.337 ± 0.097
0.148CysGln: 0.148 ± 0.05
0.316CysArg: 0.316 ± 0.083
0.569CysSer: 0.569 ± 0.104
0.548CysThr: 0.548 ± 0.109
0.654CysVal: 0.654 ± 0.121
0.105CysTrp: 0.105 ± 0.04
0.485CysTyr: 0.485 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
3.754AspAla: 3.754 ± 0.29
0.548AspCys: 0.548 ± 0.107
3.206AspAsp: 3.206 ± 0.298
4.555AspGlu: 4.555 ± 0.353
2.573AspPhe: 2.573 ± 0.217
4.028AspGly: 4.028 ± 0.312
0.928AspHis: 0.928 ± 0.153
4.26AspIle: 4.26 ± 0.292
5.314AspLys: 5.314 ± 0.41
4.682AspLeu: 4.682 ± 0.315
1.666AspMet: 1.666 ± 0.202
3.058AspAsn: 3.058 ± 0.3
2.088AspPro: 2.088 ± 0.234
1.455AspGln: 1.455 ± 0.17
2.552AspArg: 2.552 ± 0.217
3.311AspSer: 3.311 ± 0.249
3.163AspThr: 3.163 ± 0.27
4.091AspVal: 4.091 ± 0.326
0.738AspTrp: 0.738 ± 0.123
3.184AspTyr: 3.184 ± 0.247
0.0AspXaa: 0.0 ± 0.0
Glu
4.344GluAla: 4.344 ± 0.318
0.78GluCys: 0.78 ± 0.129
4.808GluAsp: 4.808 ± 0.332
7.908GluGlu: 7.908 ± 0.659
3.142GluPhe: 3.142 ± 0.26
5.04GluGly: 5.04 ± 0.358
1.645GluHis: 1.645 ± 0.191
4.956GluIle: 4.956 ± 0.35
6.348GluLys: 6.348 ± 0.424
8.056GluLeu: 8.056 ± 0.514
2.531GluMet: 2.531 ± 0.19
3.48GluAsn: 3.48 ± 0.292
2.046GluPro: 2.046 ± 0.386
3.353GluGln: 3.353 ± 0.25
3.691GluArg: 3.691 ± 0.301
3.227GluSer: 3.227 ± 0.275
3.564GluThr: 3.564 ± 0.274
5.715GluVal: 5.715 ± 0.331
0.78GluTrp: 0.78 ± 0.113
3.227GluTyr: 3.227 ± 0.266
0.0GluXaa: 0.0 ± 0.0
Phe
2.552PheAla: 2.552 ± 0.235
0.485PheCys: 0.485 ± 0.09
2.952PheAsp: 2.952 ± 0.31
2.214PheGlu: 2.214 ± 0.227
1.033PhePhe: 1.033 ± 0.136
2.299PheGly: 2.299 ± 0.207
0.738PheHis: 0.738 ± 0.126
2.278PheIle: 2.278 ± 0.225
2.931PheLys: 2.931 ± 0.249
3.522PheLeu: 3.522 ± 0.28
1.223PheMet: 1.223 ± 0.151
2.404PheAsn: 2.404 ± 0.27
1.097PhePro: 1.097 ± 0.145
1.329PheGln: 1.329 ± 0.193
1.856PheArg: 1.856 ± 0.166
2.826PheSer: 2.826 ± 0.337
2.699PheThr: 2.699 ± 0.259
2.699PheVal: 2.699 ± 0.231
0.274PheTrp: 0.274 ± 0.092
1.729PheTyr: 1.729 ± 0.205
0.0PheXaa: 0.0 ± 0.0
Gly
4.323GlyAla: 4.323 ± 0.398
0.464GlyCys: 0.464 ± 0.112
3.227GlyAsp: 3.227 ± 0.278
4.618GlyGlu: 4.618 ± 0.338
2.636GlyPhe: 2.636 ± 0.231
5.757GlyGly: 5.757 ± 0.75
1.392GlyHis: 1.392 ± 0.225
3.88GlyIle: 3.88 ± 0.269
5.104GlyLys: 5.104 ± 0.32
4.492GlyLeu: 4.492 ± 0.286
1.898GlyMet: 1.898 ± 0.269
3.416GlyAsn: 3.416 ± 0.271
0.0GlyPro: 0.0 ± 0.0
2.678GlyGln: 2.678 ± 0.249
2.826GlyArg: 2.826 ± 0.227
4.724GlySer: 4.724 ± 0.398
5.42GlyThr: 5.42 ± 0.526
4.682GlyVal: 4.682 ± 0.367
0.907GlyTrp: 0.907 ± 0.145
2.91GlyTyr: 2.91 ± 0.257
0.0GlyXaa: 0.0 ± 0.0
His
1.012HisAla: 1.012 ± 0.142
0.232HisCys: 0.232 ± 0.062
1.054HisAsp: 1.054 ± 0.179
1.392HisGlu: 1.392 ± 0.175
0.654HisPhe: 0.654 ± 0.117
1.139HisGly: 1.139 ± 0.183
0.359HisHis: 0.359 ± 0.089
1.223HisIle: 1.223 ± 0.162
1.455HisLys: 1.455 ± 0.208
1.518HisLeu: 1.518 ± 0.205
0.506HisMet: 0.506 ± 0.099
1.097HisAsn: 1.097 ± 0.157
0.738HisPro: 0.738 ± 0.13
0.569HisGln: 0.569 ± 0.104
0.991HisArg: 0.991 ± 0.141
1.097HisSer: 1.097 ± 0.184
1.033HisThr: 1.033 ± 0.158
1.392HisVal: 1.392 ± 0.182
0.274HisTrp: 0.274 ± 0.087
0.822HisTyr: 0.822 ± 0.125
0.0HisXaa: 0.0 ± 0.0
Ile
4.155IleAla: 4.155 ± 0.359
0.464IleCys: 0.464 ± 0.095
4.26IleAsp: 4.26 ± 0.301
5.272IleGlu: 5.272 ± 0.378
2.172IlePhe: 2.172 ± 0.181
4.112IleGly: 4.112 ± 0.311
1.286IleHis: 1.286 ± 0.145
3.775IleIle: 3.775 ± 0.297
5.251IleLys: 5.251 ± 0.34
4.829IleLeu: 4.829 ± 0.384
1.835IleMet: 1.835 ± 0.22
3.079IleAsn: 3.079 ± 0.297
2.784IlePro: 2.784 ± 0.249
2.425IleGln: 2.425 ± 0.184
2.552IleArg: 2.552 ± 0.215
3.923IleSer: 3.923 ± 0.277
3.944IleThr: 3.944 ± 0.365
4.534IleVal: 4.534 ± 0.329
0.464IleTrp: 0.464 ± 0.12
2.362IleTyr: 2.362 ± 0.215
0.0IleXaa: 0.0 ± 0.0
Lys
5.167LysAla: 5.167 ± 0.33
0.612LysCys: 0.612 ± 0.137
4.787LysAsp: 4.787 ± 0.328
8.457LysGlu: 8.457 ± 0.545
2.931LysPhe: 2.931 ± 0.222
4.998LysGly: 4.998 ± 0.367
1.708LysHis: 1.708 ± 0.17
3.838LysIle: 3.838 ± 0.256
7.234LysLys: 7.234 ± 0.514
6.643LysLeu: 6.643 ± 0.37
2.784LysMet: 2.784 ± 0.256
3.986LysAsn: 3.986 ± 0.271
2.72LysPro: 2.72 ± 0.203
3.817LysGln: 3.817 ± 0.308
3.923LysArg: 3.923 ± 0.328
3.859LysSer: 3.859 ± 0.277
3.817LysThr: 3.817 ± 0.282
5.568LysVal: 5.568 ± 0.326
0.78LysTrp: 0.78 ± 0.131
3.1LysTyr: 3.1 ± 0.251
0.0LysXaa: 0.0 ± 0.0
Leu
5.884LeuAla: 5.884 ± 0.301
0.443LeuCys: 0.443 ± 0.086
5.525LeuAsp: 5.525 ± 0.314
6.453LeuGlu: 6.453 ± 0.42
3.48LeuPhe: 3.48 ± 0.301
4.703LeuGly: 4.703 ± 0.314
1.329LeuHis: 1.329 ± 0.166
5.293LeuIle: 5.293 ± 0.402
6.622LeuLys: 6.622 ± 0.388
5.504LeuLeu: 5.504 ± 0.404
2.214LeuMet: 2.214 ± 0.237
3.944LeuAsn: 3.944 ± 0.279
3.142LeuPro: 3.142 ± 0.329
3.311LeuGln: 3.311 ± 0.264
4.197LeuArg: 4.197 ± 0.308
4.618LeuSer: 4.618 ± 0.319
5.125LeuThr: 5.125 ± 0.293
4.85LeuVal: 4.85 ± 0.322
1.012LeuTrp: 1.012 ± 0.145
2.995LeuTyr: 2.995 ± 0.248
0.0LeuXaa: 0.0 ± 0.0
Met
2.151MetAla: 2.151 ± 0.218
0.253MetCys: 0.253 ± 0.079
1.75MetAsp: 1.75 ± 0.207
2.299MetGlu: 2.299 ± 0.213
1.202MetPhe: 1.202 ± 0.155
1.561MetGly: 1.561 ± 0.24
0.443MetHis: 0.443 ± 0.089
2.067MetIle: 2.067 ± 0.25
2.51MetLys: 2.51 ± 0.219
2.489MetLeu: 2.489 ± 0.258
0.654MetMet: 0.654 ± 0.126
1.729MetAsn: 1.729 ± 0.189
0.78MetPro: 0.78 ± 0.13
0.991MetGln: 0.991 ± 0.152
1.539MetArg: 1.539 ± 0.193
1.645MetSer: 1.645 ± 0.202
2.109MetThr: 2.109 ± 0.223
1.308MetVal: 1.308 ± 0.163
0.401MetTrp: 0.401 ± 0.101
1.16MetTyr: 1.16 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
3.269AsnAla: 3.269 ± 0.244
0.464AsnCys: 0.464 ± 0.129
2.341AsnAsp: 2.341 ± 0.219
3.227AsnGlu: 3.227 ± 0.233
1.708AsnPhe: 1.708 ± 0.223
4.661AsnGly: 4.661 ± 0.373
0.949AsnHis: 0.949 ± 0.152
3.627AsnIle: 3.627 ± 0.278
3.901AsnLys: 3.901 ± 0.287
3.923AsnLeu: 3.923 ± 0.262
1.856AsnMet: 1.856 ± 0.219
3.016AsnAsn: 3.016 ± 0.331
2.72AsnPro: 2.72 ± 0.366
1.708AsnGln: 1.708 ± 0.171
2.91AsnArg: 2.91 ± 0.21
2.51AsnSer: 2.51 ± 0.237
3.48AsnThr: 3.48 ± 0.288
3.606AsnVal: 3.606 ± 0.277
0.801AsnTrp: 0.801 ± 0.131
2.193AsnTyr: 2.193 ± 0.213
0.0AsnXaa: 0.0 ± 0.0
Pro
2.193ProAla: 2.193 ± 0.32
0.253ProCys: 0.253 ± 0.076
1.771ProAsp: 1.771 ± 0.229
2.678ProGlu: 2.678 ± 0.308
1.265ProPhe: 1.265 ± 0.195
1.666ProGly: 1.666 ± 0.239
0.717ProHis: 0.717 ± 0.12
2.13ProIle: 2.13 ± 0.248
2.826ProLys: 2.826 ± 0.213
2.446ProLeu: 2.446 ± 0.278
0.844ProMet: 0.844 ± 0.143
2.172ProAsn: 2.172 ± 0.237
1.054ProPro: 1.054 ± 0.176
1.603ProGln: 1.603 ± 0.219
1.265ProArg: 1.265 ± 0.177
1.814ProSer: 1.814 ± 0.206
2.636ProThr: 2.636 ± 0.321
2.341ProVal: 2.341 ± 0.307
0.169ProTrp: 0.169 ± 0.084
1.413ProTyr: 1.413 ± 0.235
0.0ProXaa: 0.0 ± 0.0
Gln
3.438GlnAla: 3.438 ± 0.331
0.295GlnCys: 0.295 ± 0.075
2.214GlnAsp: 2.214 ± 0.207
3.754GlnGlu: 3.754 ± 0.296
1.392GlnPhe: 1.392 ± 0.176
2.299GlnGly: 2.299 ± 0.234
0.59GlnHis: 0.59 ± 0.133
2.362GlnIle: 2.362 ± 0.191
2.636GlnLys: 2.636 ± 0.225
3.079GlnLeu: 3.079 ± 0.235
1.392GlnMet: 1.392 ± 0.191
1.624GlnAsn: 1.624 ± 0.171
1.413GlnPro: 1.413 ± 0.26
2.193GlnGln: 2.193 ± 0.277
1.603GlnArg: 1.603 ± 0.178
2.193GlnSer: 2.193 ± 0.224
2.13GlnThr: 2.13 ± 0.201
2.847GlnVal: 2.847 ± 0.234
0.422GlnTrp: 0.422 ± 0.081
1.392GlnTyr: 1.392 ± 0.186
0.0GlnXaa: 0.0 ± 0.0
Arg
2.467ArgAla: 2.467 ± 0.244
0.337ArgCys: 0.337 ± 0.093
2.552ArgAsp: 2.552 ± 0.232
3.754ArgGlu: 3.754 ± 0.295
2.257ArgPhe: 2.257 ± 0.213
2.91ArgGly: 2.91 ± 0.245
0.78ArgHis: 0.78 ± 0.124
3.184ArgIle: 3.184 ± 0.198
3.754ArgLys: 3.754 ± 0.333
4.049ArgLeu: 4.049 ± 0.329
1.518ArgMet: 1.518 ± 0.163
2.467ArgAsn: 2.467 ± 0.284
1.223ArgPro: 1.223 ± 0.202
1.645ArgGln: 1.645 ± 0.182
1.982ArgArg: 1.982 ± 0.264
2.531ArgSer: 2.531 ± 0.212
2.214ArgThr: 2.214 ± 0.197
3.206ArgVal: 3.206 ± 0.298
0.569ArgTrp: 0.569 ± 0.11
2.088ArgTyr: 2.088 ± 0.2
0.0ArgXaa: 0.0 ± 0.0
Ser
3.669SerAla: 3.669 ± 0.299
0.422SerCys: 0.422 ± 0.09
3.29SerAsp: 3.29 ± 0.294
3.248SerGlu: 3.248 ± 0.282
2.699SerPhe: 2.699 ± 0.241
4.218SerGly: 4.218 ± 0.33
0.928SerHis: 0.928 ± 0.151
4.155SerIle: 4.155 ± 0.301
4.429SerLys: 4.429 ± 0.326
4.387SerLeu: 4.387 ± 0.262
1.455SerMet: 1.455 ± 0.203
2.952SerAsn: 2.952 ± 0.287
1.666SerPro: 1.666 ± 0.203
1.729SerGln: 1.729 ± 0.18
2.467SerArg: 2.467 ± 0.212
3.838SerSer: 3.838 ± 0.411
3.248SerThr: 3.248 ± 0.258
4.133SerVal: 4.133 ± 0.31
0.675SerTrp: 0.675 ± 0.118
2.678SerTyr: 2.678 ± 0.194
0.0SerXaa: 0.0 ± 0.0
Thr
4.028ThrAla: 4.028 ± 0.495
0.443ThrCys: 0.443 ± 0.085
3.395ThrAsp: 3.395 ± 0.286
4.007ThrGlu: 4.007 ± 0.279
2.974ThrPhe: 2.974 ± 0.271
4.703ThrGly: 4.703 ± 0.444
1.033ThrHis: 1.033 ± 0.148
3.817ThrIle: 3.817 ± 0.243
4.787ThrLys: 4.787 ± 0.34
4.808ThrLeu: 4.808 ± 0.338
1.286ThrMet: 1.286 ± 0.149
3.248ThrAsn: 3.248 ± 0.359
2.678ThrPro: 2.678 ± 0.353
2.278ThrGln: 2.278 ± 0.226
2.341ThrArg: 2.341 ± 0.248
3.395ThrSer: 3.395 ± 0.288
3.501ThrThr: 3.501 ± 0.365
4.914ThrVal: 4.914 ± 0.393
0.612ThrTrp: 0.612 ± 0.131
2.826ThrTyr: 2.826 ± 0.231
0.0ThrXaa: 0.0 ± 0.0
Val
4.893ValAla: 4.893 ± 0.352
0.612ValCys: 0.612 ± 0.127
4.344ValAsp: 4.344 ± 0.266
5.357ValGlu: 5.357 ± 0.41
2.489ValPhe: 2.489 ± 0.242
3.459ValGly: 3.459 ± 0.274
1.202ValHis: 1.202 ± 0.15
4.387ValIle: 4.387 ± 0.289
5.399ValLys: 5.399 ± 0.365
5.42ValLeu: 5.42 ± 0.339
1.561ValMet: 1.561 ± 0.164
3.691ValAsn: 3.691 ± 0.29
2.868ValPro: 2.868 ± 0.265
2.72ValGln: 2.72 ± 0.201
3.248ValArg: 3.248 ± 0.261
3.817ValSer: 3.817 ± 0.313
4.724ValThr: 4.724 ± 0.391
4.45ValVal: 4.45 ± 0.318
0.822ValTrp: 0.822 ± 0.129
3.016ValTyr: 3.016 ± 0.243
0.0ValXaa: 0.0 ± 0.0
Trp
0.654TrpAla: 0.654 ± 0.14
0.127TrpCys: 0.127 ± 0.044
0.822TrpAsp: 0.822 ± 0.134
0.844TrpGlu: 0.844 ± 0.145
0.443TrpPhe: 0.443 ± 0.108
0.696TrpGly: 0.696 ± 0.121
0.337TrpHis: 0.337 ± 0.088
0.59TrpIle: 0.59 ± 0.108
0.759TrpLys: 0.759 ± 0.13
0.949TrpLeu: 0.949 ± 0.142
0.19TrpMet: 0.19 ± 0.067
0.865TrpAsn: 0.865 ± 0.149
0.0TrpPro: 0.0 ± 0.0
0.506TrpGln: 0.506 ± 0.075
0.485TrpArg: 0.485 ± 0.087
0.675TrpSer: 0.675 ± 0.125
0.654TrpThr: 0.654 ± 0.095
0.928TrpVal: 0.928 ± 0.151
0.232TrpTrp: 0.232 ± 0.079
0.78TrpTyr: 0.78 ± 0.11
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.763TyrAla: 2.763 ± 0.294
0.359TyrCys: 0.359 ± 0.081
2.847TyrAsp: 2.847 ± 0.222
2.763TyrGlu: 2.763 ± 0.227
1.286TyrPhe: 1.286 ± 0.177
2.446TyrGly: 2.446 ± 0.233
0.886TyrHis: 0.886 ± 0.166
2.847TyrIle: 2.847 ± 0.269
3.564TyrLys: 3.564 ± 0.254
3.691TyrLeu: 3.691 ± 0.256
1.097TyrMet: 1.097 ± 0.143
2.784TyrAsn: 2.784 ± 0.201
1.223TyrPro: 1.223 ± 0.166
1.814TyrGln: 1.814 ± 0.219
2.088TyrArg: 2.088 ± 0.221
2.404TyrSer: 2.404 ± 0.196
2.742TyrThr: 2.742 ± 0.24
2.784TyrVal: 2.784 ± 0.23
0.485TyrTrp: 0.485 ± 0.118
1.645TyrTyr: 1.645 ± 0.179
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 236 proteins (47419 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski