Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Variola virus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 198 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q07037|Q07037_VAR67 C7L protein OS=Variola virus (isolate Human/India/Ind3/1967) OX=587200 GN=C7L PE=4 SV=1
MM1 pKa = 7.08YY2 pKa = 10.91NSMLPMFMCNNIVDD16 pKa = 5.42DD17 pKa = 5.2IDD19 pKa = 6.64DD20 pKa = 4.41IDD22 pKa = 6.7DD23 pKa = 4.29IDD25 pKa = 6.8DD26 pKa = 4.29IDD28 pKa = 6.8DD29 pKa = 4.29IDD31 pKa = 6.8DD32 pKa = 4.29IDD34 pKa = 6.8DD35 pKa = 4.29IDD37 pKa = 6.8DD38 pKa = 4.29IDD40 pKa = 6.8DD41 pKa = 4.29IDD43 pKa = 6.8DD44 pKa = 4.29IDD46 pKa = 6.69DD47 pKa = 4.0IDD49 pKa = 6.14DD50 pKa = 4.49IEE52 pKa = 5.78DD53 pKa = 3.43KK54 pKa = 11.2ASNNDD59 pKa = 3.04HH60 pKa = 6.62NYY62 pKa = 10.19VYY64 pKa = 10.11PLPEE68 pKa = 3.79NMVYY72 pKa = 10.57RR73 pKa = 11.84FNKK76 pKa = 7.94STNILDD82 pKa = 3.87YY83 pKa = 11.21LSTEE87 pKa = 4.1RR88 pKa = 11.84DD89 pKa = 3.57HH90 pKa = 10.24VMMAVQYY97 pKa = 10.31YY98 pKa = 8.63MSKK101 pKa = 10.26QRR103 pKa = 11.84LDD105 pKa = 3.35DD106 pKa = 4.85LYY108 pKa = 11.09RR109 pKa = 11.84QLPTKK114 pKa = 8.67TRR116 pKa = 11.84SYY118 pKa = 10.9IDD120 pKa = 4.24IINTYY125 pKa = 9.29CDD127 pKa = 3.76KK128 pKa = 11.43VNNDD132 pKa = 3.47YY133 pKa = 11.25NRR135 pKa = 11.84DD136 pKa = 3.51MNIMYY141 pKa = 10.73DD142 pKa = 3.5MASTEE147 pKa = 4.21SFTVYY152 pKa = 10.63DD153 pKa = 3.93INNEE157 pKa = 3.95VNTILMDD164 pKa = 3.83NKK166 pKa = 10.79GLGVRR171 pKa = 11.84LATISFITEE180 pKa = 3.99LGKK183 pKa = 10.52RR184 pKa = 11.84CMNPVEE190 pKa = 4.32TIKK193 pKa = 10.52MFTLLSHH200 pKa = 6.92TICDD204 pKa = 3.93DD205 pKa = 3.62CFIDD209 pKa = 4.37YY210 pKa = 8.72ITDD213 pKa = 3.57ISPPDD218 pKa = 3.36NTIPNISTRR227 pKa = 11.84EE228 pKa = 3.87YY229 pKa = 10.62LKK231 pKa = 11.04LIGITAIMFATYY243 pKa = 7.95KK244 pKa = 8.27TLKK247 pKa = 10.56YY248 pKa = 10.15MIGG251 pKa = 3.33

Molecular weight: 29.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q76R04|Q76R04_VAR67 D13_5L protein OS=Variola virus (isolate Human/India/Ind3/1967) OX=587200 GN=D13_5L PE=4 SV=1
MM1 pKa = 7.19KK2 pKa = 10.33AKK4 pKa = 10.41RR5 pKa = 11.84NLEE8 pKa = 3.78RR9 pKa = 11.84EE10 pKa = 4.14IARR13 pKa = 11.84KK14 pKa = 9.35NCEE17 pKa = 3.85GNPCEE22 pKa = 5.71RR23 pKa = 11.84EE24 pKa = 3.93LEE26 pKa = 4.24YY27 pKa = 10.97EE28 pKa = 3.9RR29 pKa = 11.84SNVKK33 pKa = 10.2RR34 pKa = 11.84LEE36 pKa = 4.1YY37 pKa = 10.58QLDD40 pKa = 3.7AEE42 pKa = 4.28KK43 pKa = 10.93EE44 pKa = 4.0KK45 pKa = 11.21VKK47 pKa = 10.42FYY49 pKa = 10.87KK50 pKa = 10.29RR51 pKa = 11.84EE52 pKa = 4.09LEE54 pKa = 3.73RR55 pKa = 11.84DD56 pKa = 3.35RR57 pKa = 11.84YY58 pKa = 10.44LSSRR62 pKa = 11.84YY63 pKa = 8.1LTSSSDD69 pKa = 3.31PHH71 pKa = 6.52KK72 pKa = 10.57KK73 pKa = 9.65HH74 pKa = 5.54YY75 pKa = 10.34QIIHH79 pKa = 5.91FLALKK84 pKa = 9.69MYY86 pKa = 10.55LRR88 pKa = 11.84RR89 pKa = 11.84QLRR92 pKa = 11.84LQVLL96 pKa = 4.01

Molecular weight: 11.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

189

9

198

54086

40

1896

273.2

31.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.753 ± 0.114

2.1 ± 0.1

6.554 ± 0.142

5.325 ± 0.139

4.672 ± 0.145

3.966 ± 0.113

2.071 ± 0.089

9.404 ± 0.163

7.427 ± 0.112

8.829 ± 0.163

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.095

7.039 ± 0.118

3.341 ± 0.116

2.289 ± 0.078

4.26 ± 0.108

7.813 ± 0.156

6.216 ± 0.155

6.162 ± 0.114

0.682 ± 0.044

5.327 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski