BtMf-AlphaCoV/JX2012

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Minunacovirus; Miniopterus bat coronavirus 1

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1WHB9|A0A0U1WHB9_9ALPC Envelope small membrane protein OS=BtMf-AlphaCoV/JX2012 OX=1503288 GN=E PE=3 SV=1
MM1 pKa = 7.44FIIYY5 pKa = 9.43FCVALCINFASANIGCSSNGNLGMYY30 pKa = 9.8RR31 pKa = 11.84LKK33 pKa = 10.91LGLPNNVTNAYY44 pKa = 9.78VSGYY48 pKa = 10.59LPTPDD53 pKa = 4.14QWNCTQNSAGTANITYY69 pKa = 9.49NARR72 pKa = 11.84AVFISYY78 pKa = 10.44YY79 pKa = 10.66KK80 pKa = 10.44FGTGVAVGVGNSKK93 pKa = 11.17SNDD96 pKa = 2.93SFGIYY101 pKa = 10.01FYY103 pKa = 11.02HH104 pKa = 7.37HH105 pKa = 6.81NGEE108 pKa = 4.06QKK110 pKa = 9.88TILRR114 pKa = 11.84VCKK117 pKa = 9.73WNGRR121 pKa = 11.84YY122 pKa = 8.23MPSYY126 pKa = 10.52RR127 pKa = 11.84PTSTTNGYY135 pKa = 10.5DD136 pKa = 3.21CVINVNLNLRR146 pKa = 11.84FVDD149 pKa = 3.87QQSHH153 pKa = 5.85NDD155 pKa = 3.33VFGLSWSGDD164 pKa = 3.05RR165 pKa = 11.84LIIYY169 pKa = 10.05SVDD172 pKa = 3.21GVSSHH177 pKa = 6.6HH178 pKa = 6.76VDD180 pKa = 4.42GIGDD184 pKa = 4.25LDD186 pKa = 3.82VVSFRR191 pKa = 11.84CQLKK195 pKa = 7.94EE196 pKa = 4.08TCAHH200 pKa = 5.15QVVTKK205 pKa = 10.16PITAIVNTTSDD216 pKa = 3.69GFISGYY222 pKa = 7.31EE223 pKa = 3.86VCADD227 pKa = 3.28CDD229 pKa = 3.83GFPKK233 pKa = 10.72YY234 pKa = 10.73VFAVTDD240 pKa = 4.16GGAIPPNFQFDD251 pKa = 2.88NWFYY255 pKa = 10.18LTNSSTPISGMFTSVQPFNLSCVWPVPVLTSNSLPVHH292 pKa = 6.5FNMSLNNGAHH302 pKa = 6.45CNGYY306 pKa = 10.78SNLDD310 pKa = 3.5VGLVDD315 pKa = 5.3AMRR318 pKa = 11.84FSLNFTDD325 pKa = 3.65SQIIRR330 pKa = 11.84TGVISVITSTNVFNFSCTNSSVFGEE355 pKa = 4.38TNVIPFGDD363 pKa = 3.69VEE365 pKa = 4.23KK366 pKa = 9.67THH368 pKa = 6.5YY369 pKa = 10.86CFINYY374 pKa = 6.99FTNITTGEE382 pKa = 4.2SASQFVGILPPQVKK396 pKa = 9.71EE397 pKa = 3.69FVIMRR402 pKa = 11.84NGDD405 pKa = 3.34FHH407 pKa = 8.25LNGYY411 pKa = 10.09RR412 pKa = 11.84IFSVDD417 pKa = 3.14SVEE420 pKa = 4.69SVIFNISTKK429 pKa = 10.3DD430 pKa = 3.43GRR432 pKa = 11.84DD433 pKa = 3.2FWTVAFANNAEE444 pKa = 4.17VLTEE448 pKa = 4.01INATSIQNLLYY459 pKa = 10.82CNNPVNSIKK468 pKa = 10.55CQQLRR473 pKa = 11.84FNLDD477 pKa = 3.27DD478 pKa = 4.0GFYY481 pKa = 10.69SHH483 pKa = 7.19TFDD486 pKa = 5.35SVDD489 pKa = 3.28NLPRR493 pKa = 11.84TIVRR497 pKa = 11.84LPKK500 pKa = 10.35YY501 pKa = 8.13VTHH504 pKa = 6.86SIINVTVGINFEE516 pKa = 4.41NKK518 pKa = 8.8TLTNYY523 pKa = 10.83LIDD526 pKa = 4.39FAPDD530 pKa = 3.28DD531 pKa = 4.12VSIGKK536 pKa = 9.88DD537 pKa = 3.19NITTVCVDD545 pKa = 3.16TTTFTVRR552 pKa = 11.84LNVLAFSSFSTAVDD566 pKa = 3.57IQAGTCPFSYY576 pKa = 11.29NNLNNYY582 pKa = 9.99LGFGSLCFSTRR593 pKa = 11.84PNGGCMMSVVARR605 pKa = 11.84GYY607 pKa = 9.89FGEE610 pKa = 4.14FQKK613 pKa = 11.28VGVLYY618 pKa = 11.01VSFTKK623 pKa = 10.63GDD625 pKa = 3.57NVLGVPPNNVPTIGVSDD642 pKa = 3.7MSDD645 pKa = 2.83VKK647 pKa = 11.15LGVCTTFTIYY657 pKa = 10.65GHH659 pKa = 5.54TGRR662 pKa = 11.84GIINKK667 pKa = 9.73SNNTFVSGLFYY678 pKa = 10.84TSVTGNLLGFKK689 pKa = 10.45NSTTGEE695 pKa = 4.0IFSISPCQLTTQVAVISDD713 pKa = 4.37KK714 pKa = 11.0IVGVAAATTEE724 pKa = 4.1VKK726 pKa = 10.74LPFDD730 pKa = 4.24NYY732 pKa = 9.98VSLGSFYY739 pKa = 10.56YY740 pKa = 10.02HH741 pKa = 6.21YY742 pKa = 10.07RR743 pKa = 11.84NSSAEE748 pKa = 3.92LCKK751 pKa = 10.85NPSLMYY757 pKa = 10.57GGLGVCEE764 pKa = 5.02DD765 pKa = 3.57GRR767 pKa = 11.84LVNISRR773 pKa = 11.84SEE775 pKa = 4.02DD776 pKa = 3.45TFVPSAVISGNITIPANFSFVVQPEE801 pKa = 4.31YY802 pKa = 10.43IQIMTKK808 pKa = 9.87PVSVDD813 pKa = 2.99CSVYY817 pKa = 10.26VCNGNPRR824 pKa = 11.84CLQLLTQYY832 pKa = 11.57ASVCRR837 pKa = 11.84TVEE840 pKa = 4.29EE841 pKa = 4.13PLQLNARR848 pKa = 11.84LEE850 pKa = 4.16ALEE853 pKa = 4.37LTSMIIVQDD862 pKa = 3.48KK863 pKa = 8.43TLKK866 pKa = 10.86LGVASNFNDD875 pKa = 3.82TFDD878 pKa = 3.91LTLALPRR885 pKa = 11.84QHH887 pKa = 6.45QSRR890 pKa = 11.84SAIEE894 pKa = 4.54DD895 pKa = 3.65LLFSKK900 pKa = 10.43IVTSGLGTVDD910 pKa = 5.34DD911 pKa = 5.32DD912 pKa = 4.97YY913 pKa = 11.92KK914 pKa = 11.26EE915 pKa = 4.45CAAKK919 pKa = 9.16MANTIAEE926 pKa = 4.49AGCVQYY932 pKa = 11.5YY933 pKa = 9.78NGIMVLPGVVDD944 pKa = 4.33PSLLSQYY951 pKa = 9.05TAALTGAMVLGGVTAAAAIPFSLAVQARR979 pKa = 11.84LNYY982 pKa = 10.03LALQTDD988 pKa = 4.14VLQRR992 pKa = 11.84NQMILAQSFNAAMGNITVAFSGVSNAIQQTAQSLNTISQALNKK1035 pKa = 9.73VEE1037 pKa = 4.27NVVNEE1042 pKa = 3.91QGTALSQLTKK1052 pKa = 10.61QLASNFQAISSSIEE1066 pKa = 3.69DD1067 pKa = 3.97LYY1069 pKa = 11.84NRR1071 pKa = 11.84LDD1073 pKa = 3.6TLEE1076 pKa = 5.38ADD1078 pKa = 3.74QQVDD1082 pKa = 3.46RR1083 pKa = 11.84LITGRR1088 pKa = 11.84LAALNAFVTQQLLRR1102 pKa = 11.84YY1103 pKa = 8.9SEE1105 pKa = 4.28VRR1107 pKa = 11.84ASRR1110 pKa = 11.84QLAQEE1115 pKa = 4.75KK1116 pKa = 10.45INEE1119 pKa = 4.44CVKK1122 pKa = 10.53SQSSRR1127 pKa = 11.84YY1128 pKa = 8.35GFCGNGTHH1136 pKa = 5.87VFSVANAAPDD1146 pKa = 4.45GIMFLHH1152 pKa = 6.91ANLVPTAFIEE1162 pKa = 4.41VSAFAGVCVEE1172 pKa = 3.85GRR1174 pKa = 11.84ALVLRR1179 pKa = 11.84GRR1181 pKa = 11.84DD1182 pKa = 3.0EE1183 pKa = 4.97VLFQKK1188 pKa = 10.29PNTMQYY1194 pKa = 10.94LITPRR1199 pKa = 11.84LLFEE1203 pKa = 4.25PRR1205 pKa = 11.84VPVSADD1211 pKa = 3.25FVEE1214 pKa = 4.87VSSCNVTFINLTINEE1229 pKa = 4.53LPEE1232 pKa = 4.46LLPDD1236 pKa = 4.18YY1237 pKa = 10.98IDD1239 pKa = 3.45VNKK1242 pKa = 9.25TLKK1245 pKa = 10.34EE1246 pKa = 3.88FAATIPNRR1254 pKa = 11.84TEE1256 pKa = 3.59MQLTLNTYY1264 pKa = 9.78NATVLNLTDD1273 pKa = 3.74EE1274 pKa = 4.85VKK1276 pKa = 10.99SLMSQADD1283 pKa = 4.45DD1284 pKa = 3.54LTKK1287 pKa = 10.24IASEE1291 pKa = 4.28LNLTISKK1298 pKa = 10.27INNTLVEE1305 pKa = 4.51LEE1307 pKa = 3.89WLNRR1311 pKa = 11.84VEE1313 pKa = 5.97TYY1315 pKa = 10.4IKK1317 pKa = 9.3WPWYY1321 pKa = 7.96VWLAIAVTLIILVGLMLWCCLATGCCGCCSCLVNSCSDD1359 pKa = 3.33CGGRR1363 pKa = 11.84RR1364 pKa = 11.84LQRR1367 pKa = 11.84YY1368 pKa = 8.12EE1369 pKa = 3.64IEE1371 pKa = 4.3KK1372 pKa = 10.33VHH1374 pKa = 5.94IQQ1376 pKa = 3.19

Molecular weight: 150.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1WHB7|A0A0U1WHB7_9ALPC Spike glycoprotein OS=BtMf-AlphaCoV/JX2012 OX=1503288 PE=4 SV=1
MM1 pKa = 7.75ASVQFEE7 pKa = 4.47DD8 pKa = 3.64TNSRR12 pKa = 11.84GRR14 pKa = 11.84SGRR17 pKa = 11.84IPLSYY22 pKa = 9.62YY23 pKa = 10.63APVRR27 pKa = 11.84VADD30 pKa = 4.65DD31 pKa = 3.83KK32 pKa = 11.82LFLKK36 pKa = 10.66SMPGNGVPKK45 pKa = 10.76GMGGKK50 pKa = 9.77AEE52 pKa = 4.14QIGYY56 pKa = 6.34WTEE59 pKa = 3.6QRR61 pKa = 11.84RR62 pKa = 11.84WRR64 pKa = 11.84MNKK67 pKa = 8.32GQRR70 pKa = 11.84KK71 pKa = 8.92DD72 pKa = 4.4LPSNWHH78 pKa = 6.54FYY80 pKa = 10.83YY81 pKa = 10.82LGTGPHH87 pKa = 6.91ADD89 pKa = 2.92LKK91 pKa = 10.62FRR93 pKa = 11.84EE94 pKa = 4.21RR95 pKa = 11.84QVGVFWVAKK104 pKa = 9.45QGSKK108 pKa = 9.38TEE110 pKa = 4.03PTNLGTRR117 pKa = 11.84NRR119 pKa = 11.84NQQLRR124 pKa = 11.84IPEE127 pKa = 4.11FDD129 pKa = 3.1FTLPDD134 pKa = 3.28SLEE137 pKa = 4.22VVNSDD142 pKa = 2.99SRR144 pKa = 11.84PASRR148 pKa = 11.84SNSRR152 pKa = 11.84SQSKK156 pKa = 10.82SNNQSRR162 pKa = 11.84SNSKK166 pKa = 10.5ARR168 pKa = 11.84DD169 pKa = 3.42QSGTRR174 pKa = 11.84TPKK177 pKa = 10.4NASQNQKK184 pKa = 9.64PKK186 pKa = 10.77KK187 pKa = 9.04NQQEE191 pKa = 4.18DD192 pKa = 3.64LVAAVRR198 pKa = 11.84EE199 pKa = 4.15ALKK202 pKa = 11.01GLGIQQSRR210 pKa = 11.84SSSGKK215 pKa = 8.13STPARR220 pKa = 11.84SKK222 pKa = 11.13SPARR226 pKa = 11.84PSNKK230 pKa = 9.31KK231 pKa = 9.84QLDD234 pKa = 3.49KK235 pKa = 11.31PEE237 pKa = 4.08WKK239 pKa = 9.82RR240 pKa = 11.84VPNRR244 pKa = 11.84TEE246 pKa = 3.54NATTCFGQRR255 pKa = 11.84SVEE258 pKa = 4.2RR259 pKa = 11.84NCGSASVVGMGVEE272 pKa = 4.43APNFPQIAEE281 pKa = 4.98LIPTQAALFFGSRR294 pKa = 11.84VSTKK298 pKa = 10.23EE299 pKa = 3.51VGDD302 pKa = 3.76TVEE305 pKa = 4.23IKK307 pKa = 10.26YY308 pKa = 9.74HH309 pKa = 5.79YY310 pKa = 10.05KK311 pKa = 9.87MSVPKK316 pKa = 9.99TNPNLPYY323 pKa = 10.51FLQQVNAYY331 pKa = 9.7LDD333 pKa = 4.12PNSDD337 pKa = 3.17QPIPKK342 pKa = 9.52KK343 pKa = 10.36PKK345 pKa = 9.69AKK347 pKa = 10.09PEE349 pKa = 3.99EE350 pKa = 4.21VPQLNPAAPVFTPPAQEE367 pKa = 3.78PAVEE371 pKa = 4.41LEE373 pKa = 4.26MVDD376 pKa = 3.64EE377 pKa = 4.78VFDD380 pKa = 4.29IDD382 pKa = 4.1PLGDD386 pKa = 3.55SVAA389 pKa = 3.81

Molecular weight: 43.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

9241

74

6931

1540.2

171.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.59 ± 0.216

3.344 ± 0.632

5.497 ± 0.792

4.123 ± 0.29

5.811 ± 0.288

6.471 ± 0.278

1.85 ± 0.256

5.378 ± 0.538

5.681 ± 1.041

8.224 ± 0.883

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.067 ± 0.198

6.298 ± 0.564

3.495 ± 0.76

2.77 ± 0.74

3.333 ± 0.553

7.478 ± 0.338

5.995 ± 0.675

10.042 ± 0.684

1.104 ± 0.277

4.448 ± 0.386
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski