Mycobacterium phage C3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5XBM6|A0A2Z5XBM6_9CAUD Uncharacterized protein OS=Mycobacterium phage C3 OX=2072005 PE=4 SV=1
MM1 pKa = 7.33TLDD4 pKa = 3.48EE5 pKa = 5.79LIAKK9 pKa = 7.98LTEE12 pKa = 3.69IRR14 pKa = 11.84DD15 pKa = 3.67EE16 pKa = 4.59HH17 pKa = 6.94GGSLLVTRR25 pKa = 11.84WNTCCCYY32 pKa = 10.9GDD34 pKa = 3.92WEE36 pKa = 4.4VDD38 pKa = 3.53VTDD41 pKa = 3.68VEE43 pKa = 4.41FDD45 pKa = 3.51GGVLISS51 pKa = 3.79

Molecular weight: 5.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5XBI8|A0A2Z5XBI8_9CAUD Putative phosphoesterase OS=Mycobacterium phage C3 OX=2072005 PE=4 SV=1
MM1 pKa = 7.49RR2 pKa = 11.84RR3 pKa = 11.84VLITGSRR10 pKa = 11.84VWKK13 pKa = 9.85DD14 pKa = 3.33RR15 pKa = 11.84SMVWGALANEE25 pKa = 4.63LRR27 pKa = 11.84RR28 pKa = 11.84SPSGLVVVHH37 pKa = 6.42GGARR41 pKa = 11.84GADD44 pKa = 3.88DD45 pKa = 5.02IADD48 pKa = 3.67RR49 pKa = 11.84WAWGKK54 pKa = 9.6RR55 pKa = 11.84QEE57 pKa = 4.65GYY59 pKa = 10.86NVTPEE64 pKa = 3.93LHH66 pKa = 6.27RR67 pKa = 11.84ADD69 pKa = 3.13WDD71 pKa = 4.03RR72 pKa = 11.84YY73 pKa = 8.3GKK75 pKa = 10.32RR76 pKa = 11.84AGILRR81 pKa = 11.84NIEE84 pKa = 4.06MVQAGADD91 pKa = 3.41LCLAFPLGNSVGTRR105 pKa = 11.84HH106 pKa = 6.41CMRR109 pKa = 11.84EE110 pKa = 4.02AEE112 pKa = 4.19RR113 pKa = 11.84AGIPVVNLGDD123 pKa = 3.53RR124 pKa = 11.84SS125 pKa = 3.53

Molecular weight: 13.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

15687

31

837

178.3

19.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.76 ± 0.339

0.95 ± 0.135

6.477 ± 0.212

7.031 ± 0.284

3.481 ± 0.166

8.134 ± 0.435

2.136 ± 0.164

4.864 ± 0.181

4.564 ± 0.232

8.179 ± 0.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.117

3.321 ± 0.151

5.291 ± 0.234

3.461 ± 0.194

6.642 ± 0.36

5.253 ± 0.204

5.846 ± 0.194

7.235 ± 0.206

2.11 ± 0.121

2.856 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski