Staphylococcus phage SA345ruMSSAST8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Biseptimavirus; Staphylococcus virus SA345ruMSSAST8

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345AQZ9|A0A345AQZ9_9CAUD Uncharacterized protein OS=Staphylococcus phage SA345ruMSSAST8 OX=2268621 PE=4 SV=1
MM1 pKa = 6.5MPKK4 pKa = 9.89YY5 pKa = 10.24RR6 pKa = 11.84VWDD9 pKa = 3.82KK10 pKa = 9.61YY11 pKa = 8.51TGRR14 pKa = 11.84IHH16 pKa = 7.36DD17 pKa = 3.5VVGFDD22 pKa = 4.3FIEE25 pKa = 4.49TEE27 pKa = 4.02VHH29 pKa = 5.32YY30 pKa = 10.72EE31 pKa = 3.88NYY33 pKa = 10.82AEE35 pKa = 4.68AEE37 pKa = 4.19ALIHH41 pKa = 7.04ARR43 pKa = 11.84DD44 pKa = 3.87FKK46 pKa = 11.16DD47 pKa = 3.46VEE49 pKa = 4.44LMQSTGLKK57 pKa = 10.18DD58 pKa = 3.37KK59 pKa = 11.2NNNEE63 pKa = 3.97IYY65 pKa = 10.76AGDD68 pKa = 3.32IVEE71 pKa = 5.19FEE73 pKa = 4.4DD74 pKa = 5.71EE75 pKa = 4.21ILEE78 pKa = 4.36MPDD81 pKa = 3.56DD82 pKa = 4.11EE83 pKa = 5.64SVIGTINRR91 pKa = 11.84AVISIDD97 pKa = 3.39VVNGIQLKK105 pKa = 10.93DD106 pKa = 3.87FMFEE110 pKa = 4.12GAVSEE115 pKa = 4.04NDD117 pKa = 3.09YY118 pKa = 11.39FEE120 pKa = 5.48YY121 pKa = 10.44IDD123 pKa = 3.3IKK125 pKa = 11.24SFLRR129 pKa = 11.84YY130 pKa = 9.84DD131 pKa = 3.91CEE133 pKa = 4.24VKK135 pKa = 11.01GNIFEE140 pKa = 4.37SSHH143 pKa = 7.01LLEE146 pKa = 4.22VTEE149 pKa = 4.4

Molecular weight: 17.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345AR28|A0A345AR28_9CAUD Holin OS=Staphylococcus phage SA345ruMSSAST8 OX=2268621 PE=4 SV=1
MM1 pKa = 7.2VALLKK6 pKa = 10.65SLEE9 pKa = 3.97RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84LMITISTMLQFGLFLIALIGLVIKK36 pKa = 10.76LIEE39 pKa = 4.3LSNKK43 pKa = 9.19KK44 pKa = 10.07

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12525

44

1509

195.7

22.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.525 ± 0.409

0.551 ± 0.101

6.267 ± 0.323

8.24 ± 0.522

4.311 ± 0.283

5.533 ± 0.604

1.645 ± 0.167

8.096 ± 0.282

9.661 ± 0.337

7.944 ± 0.268

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.195

6.778 ± 0.318

2.539 ± 0.175

3.808 ± 0.258

4.439 ± 0.233

5.693 ± 0.264

5.461 ± 0.234

5.605 ± 0.23

1.118 ± 0.148

4.359 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski