Eclipta yellow vein virus
Average proteome isoelectric point is 7.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M4ATQ7|A0A0M4ATQ7_9GEMI Replication-associated protein OS=Eclipta yellow vein virus OX=875324 GN=AC1 PE=3 SV=1
MM1 pKa = 8.06 WDD3 pKa = 3.35 PLLNEE8 pKa = 4.38 FPEE11 pKa = 4.68 TVHH14 pKa = 7.0 GFRR17 pKa = 11.84 CMLAVKK23 pKa = 9.91 YY24 pKa = 9.14 LQQLSEE30 pKa = 4.6 EE31 pKa = 4.3 YY32 pKa = 10.92 SPDD35 pKa = 3.21 TVGYY39 pKa = 10.56 DD40 pKa = 3.97 LIRR43 pKa = 11.84 DD44 pKa = 4.54 LICILRR50 pKa = 11.84 SRR52 pKa = 11.84 NYY54 pKa = 9.65 VEE56 pKa = 4.26 ASCRR60 pKa = 11.84 YY61 pKa = 7.36 RR62 pKa = 11.84 HH63 pKa = 6.19 FYY65 pKa = 10.95 ARR67 pKa = 11.84 VEE69 pKa = 4.15 STPASEE75 pKa = 4.25 LRR77 pKa = 11.84 QPIHH81 pKa = 6.33 QPCCCPHH88 pKa = 6.78 CPPPKK93 pKa = 8.72 ISHH96 pKa = 6.17 VGEE99 pKa = 4.01 QAHH102 pKa = 6.28 EE103 pKa = 4.35 SEE105 pKa = 4.4 AHH107 pKa = 5.7 NVQDD111 pKa = 3.58 VQKK114 pKa = 10.15 PP115 pKa = 3.38
Molecular weight: 13.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.416
IPC2_protein 5.626
IPC_protein 5.652
Toseland 6.02
ProMoST 6.02
Dawson 5.931
Bjellqvist 5.931
Wikipedia 5.944
Rodwell 5.931
Grimsley 6.148
Solomon 5.931
Lehninger 5.931
Nozaki 6.211
DTASelect 6.389
Thurlkill 6.415
EMBOSS 6.376
Sillero 6.313
Patrickios 1.265
IPC_peptide 5.957
IPC2_peptide 6.338
IPC2.peptide.svr19 6.197
Protein with the highest isoelectric point:
>tr|A0A0M4AKS6|A0A0M4AKS6_9GEMI AC4 protein OS=Eclipta yellow vein virus OX=875324 GN=AC4 PE=3 SV=1
MM1 pKa = 8.08 DD2 pKa = 5.22 LRR4 pKa = 11.84 TGEE7 pKa = 5.09 PITAAQAWSGAYY19 pKa = 7.65 TWEE22 pKa = 4.36 VPNPLYY28 pKa = 10.63 FKK30 pKa = 10.52 ILSHH34 pKa = 7.01 DD35 pKa = 3.64 NRR37 pKa = 11.84 PFTSKK42 pKa = 10.08 MDD44 pKa = 4.22 IITIRR49 pKa = 11.84 IQFNYY54 pKa = 9.82 NLRR57 pKa = 11.84 RR58 pKa = 11.84 ALGVHH63 pKa = 5.82 KK64 pKa = 10.54 CFLTFRR70 pKa = 11.84 VWTTLHH76 pKa = 6.82 PPTGLFLRR84 pKa = 11.84 VFKK87 pKa = 9.93 TQVLKK92 pKa = 11.01 YY93 pKa = 9.9 LNNLGVISINAIIRR107 pKa = 11.84 AVDD110 pKa = 3.21 HH111 pKa = 6.21 VLWKK115 pKa = 10.63 KK116 pKa = 10.8 LEE118 pKa = 3.8 QTVYY122 pKa = 10.69 VNQDD126 pKa = 3.08 SEE128 pKa = 4.68 IKK130 pKa = 10.48 FNLYY134 pKa = 10.23
Molecular weight: 15.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.167
IPC2_protein 9.282
IPC_protein 9.326
Toseland 9.794
ProMoST 9.619
Dawson 10.058
Bjellqvist 9.765
Wikipedia 10.248
Rodwell 10.35
Grimsley 10.145
Solomon 10.087
Lehninger 10.058
Nozaki 9.794
DTASelect 9.75
Thurlkill 9.882
EMBOSS 10.204
Sillero 9.97
Patrickios 7.585
IPC_peptide 10.087
IPC2_peptide 8.434
IPC2.peptide.svr19 8.293
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1130
112
363
188.3
21.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.575 ± 0.952
2.478 ± 0.656
4.779 ± 0.264
5.044 ± 0.972
4.513 ± 0.489
4.956 ± 0.542
3.628 ± 0.566
5.398 ± 0.503
5.487 ± 0.65
7.345 ± 0.927
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.858 ± 0.536
5.929 ± 0.548
6.372 ± 0.572
5.221 ± 0.93
6.106 ± 0.709
8.053 ± 1.371
5.664 ± 0.935
5.929 ± 1.312
1.681 ± 0.228
3.982 ± 0.503
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here