Acetomicrobium mobile (strain ATCC BAA-54 / DSM 13181 / JCM 12221 / NGA) (Acetomicrobium mobile)
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2004 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I4BWK4|I4BWK4_ACEMN L-asparaginase/GlutRNAGln amidotransferase subunit D OS=Acetomicrobium mobile (strain ATCC BAA-54 / DSM 13181 / JCM 12221 / NGA) OX=891968 GN=Anamo_1036 PE=3 SV=1
MM1 pKa = 7.5 SKK3 pKa = 10.65 LVTCIVCEE11 pKa = 4.21 GKK13 pKa = 9.74 IQVSDD18 pKa = 4.06 DD19 pKa = 3.78 LLEE22 pKa = 4.45 GEE24 pKa = 5.39 IIVCPDD30 pKa = 3.44 CGSEE34 pKa = 4.37 LEE36 pKa = 4.55 VVSLDD41 pKa = 3.58 PLTLEE46 pKa = 4.43 EE47 pKa = 4.3 APEE50 pKa = 4.0 VQEE53 pKa = 4.76 DD54 pKa = 3.23 WGEE57 pKa = 3.77
Molecular weight: 6.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.935
IPC2_protein 3.567
IPC_protein 3.414
Toseland 3.261
ProMoST 3.567
Dawson 3.414
Bjellqvist 3.681
Wikipedia 3.338
Rodwell 3.287
Grimsley 3.198
Solomon 3.35
Lehninger 3.3
Nozaki 3.567
DTASelect 3.643
Thurlkill 3.338
EMBOSS 3.35
Sillero 3.554
Patrickios 0.477
IPC_peptide 3.35
IPC2_peptide 3.516
IPC2.peptide.svr19 3.651
Protein with the highest isoelectric point:
>tr|I4BVH8|I4BVH8_ACEMN ABC-type transport system involved in resistance to organic solvents ATPase component OS=Acetomicrobium mobile (strain ATCC BAA-54 / DSM 13181 / JCM 12221 / NGA) OX=891968 GN=Anamo_0636 PE=4 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.04 RR4 pKa = 11.84 TFQPHH9 pKa = 4.54 VKK11 pKa = 9.17 PRR13 pKa = 11.84 KK14 pKa = 8.96 RR15 pKa = 11.84 KK16 pKa = 8.27 MGFLARR22 pKa = 11.84 TSSPSGRR29 pKa = 11.84 RR30 pKa = 11.84 ILRR33 pKa = 11.84 NRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.17 GRR40 pKa = 11.84 QRR42 pKa = 11.84 LAVV45 pKa = 3.51
Molecular weight: 5.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2004
0
2004
649885
29
1653
324.3
35.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.237 ± 0.051
1.336 ± 0.022
5.36 ± 0.042
6.879 ± 0.056
4.069 ± 0.037
7.933 ± 0.052
1.734 ± 0.021
7.116 ± 0.05
6.119 ± 0.048
10.475 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.821 ± 0.023
3.265 ± 0.027
4.359 ± 0.033
2.553 ± 0.028
5.352 ± 0.038
5.982 ± 0.042
4.385 ± 0.033
7.774 ± 0.041
1.183 ± 0.023
3.07 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here