Beihai tombus-like virus 19

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KFJ6|A0A1L3KFJ6_9VIRU RNA-directed RNA polymerase OS=Beihai tombus-like virus 19 OX=1922722 PE=4 SV=1
MM1 pKa = 7.81LLPPFLSRR9 pKa = 11.84TIALPARR16 pKa = 11.84TVEE19 pKa = 3.98NQIAGIEE26 pKa = 4.06KK27 pKa = 9.85RR28 pKa = 11.84VLAVVPQPHH37 pKa = 7.61PDD39 pKa = 3.43LDD41 pKa = 3.61PWNDD45 pKa = 3.19EE46 pKa = 3.67ASPYY50 pKa = 8.93HH51 pKa = 6.3TDD53 pKa = 3.18RR54 pKa = 11.84LALPRR59 pKa = 11.84EE60 pKa = 3.88RR61 pKa = 11.84LGVVEE66 pKa = 5.62EE67 pKa = 4.46DD68 pKa = 5.09LEE70 pKa = 4.62DD71 pKa = 3.64LTALLSSQRR80 pKa = 11.84QEE82 pKa = 3.68HH83 pKa = 6.4RR84 pKa = 11.84EE85 pKa = 3.66RR86 pKa = 11.84ALRR89 pKa = 11.84LVDD92 pKa = 3.75LAWRR96 pKa = 11.84RR97 pKa = 11.84RR98 pKa = 11.84RR99 pKa = 11.84GAEE102 pKa = 3.87LARR105 pKa = 11.84SEE107 pKa = 4.26LPPWEE112 pKa = 4.27ARR114 pKa = 11.84GQRR117 pKa = 11.84RR118 pKa = 11.84GRR120 pKa = 11.84LNSRR124 pKa = 11.84EE125 pKa = 3.92ILVSANLPCLSRR137 pKa = 11.84LRR139 pKa = 11.84DD140 pKa = 3.56QDD142 pKa = 3.79CLEE145 pKa = 3.86VHH147 pKa = 6.23QRR149 pKa = 11.84KK150 pKa = 8.36LWLLPSLLRR159 pKa = 11.84LRR161 pKa = 11.84DD162 pKa = 3.65PRR164 pKa = 11.84SSGVAVRR171 pKa = 11.84VFPAMSWEE179 pKa = 4.4TFCDD183 pKa = 3.49EE184 pKa = 4.85SPRR187 pKa = 11.84LRR189 pKa = 11.84DD190 pKa = 3.43WVPSRR195 pKa = 11.84LGDD198 pKa = 3.83SQDD201 pKa = 3.6PEE203 pKa = 4.4HH204 pKa = 6.75ALSRR208 pKa = 11.84PAHH211 pKa = 6.3RR212 pKa = 11.84PLFSNTPHH220 pKa = 6.87ARR222 pKa = 11.84QICRR226 pKa = 11.84NSWGTCPCLTSTQLLTSVSTYY247 pKa = 9.84RR248 pKa = 11.84QCLLPLPTTLCRR260 pKa = 11.84PPPSTTLVEE269 pKa = 4.15PVVLVLPVHH278 pKa = 7.14LIRR281 pKa = 11.84HH282 pKa = 6.62LGPKK286 pKa = 10.0DD287 pKa = 3.54VGSDD291 pKa = 3.38LPEE294 pKa = 4.09FSPRR298 pKa = 11.84FAEE301 pKa = 4.34DD302 pKa = 3.84LEE304 pKa = 4.82DD305 pKa = 3.82SGRR308 pKa = 11.84KK309 pKa = 9.12LLEE312 pKa = 4.53HH313 pKa = 7.36EE314 pKa = 5.0DD315 pKa = 3.81FQFLTRR321 pKa = 11.84SKK323 pKa = 10.46PLEE326 pKa = 3.98FEE328 pKa = 4.65DD329 pKa = 3.49WVKK332 pKa = 10.6RR333 pKa = 11.84YY334 pKa = 8.59PPHH337 pKa = 6.61RR338 pKa = 11.84RR339 pKa = 11.84EE340 pKa = 4.06EE341 pKa = 4.0LRR343 pKa = 11.84EE344 pKa = 3.99ARR346 pKa = 11.84TKK348 pKa = 10.88ALRR351 pKa = 11.84NGISPKK357 pKa = 10.34DD358 pKa = 3.57ANLKK362 pKa = 9.98VFMKK366 pKa = 10.42IEE368 pKa = 4.18GTTTGTDD375 pKa = 3.28PRR377 pKa = 11.84NISPRR382 pKa = 11.84TDD384 pKa = 2.8AFLSIMGPYY393 pKa = 9.02VAGIEE398 pKa = 4.15HH399 pKa = 7.14AAVQEE404 pKa = 4.14PRR406 pKa = 11.84CPFLVKK412 pKa = 10.64GMDD415 pKa = 2.98VSTRR419 pKa = 11.84GSYY422 pKa = 10.86LSRR425 pKa = 11.84TDD427 pKa = 2.77KK428 pKa = 10.91GARR431 pKa = 11.84AEE433 pKa = 4.09IDD435 pKa = 3.56FSRR438 pKa = 11.84FDD440 pKa = 3.44QSLSTAVLQAFEE452 pKa = 4.16LAAFRR457 pKa = 11.84VAYY460 pKa = 9.66PEE462 pKa = 4.35GLHH465 pKa = 6.73PLLDD469 pKa = 3.78EE470 pKa = 5.2CLACLDD476 pKa = 3.85EE477 pKa = 4.74MKK479 pKa = 10.68GFSDD483 pKa = 3.8LGVSYY488 pKa = 10.34RR489 pKa = 11.84VEE491 pKa = 3.94ATRR494 pKa = 11.84ASGDD498 pKa = 3.04AHH500 pKa = 5.49TSIMNGVDD508 pKa = 3.69NRR510 pKa = 11.84CLCQYY515 pKa = 10.8VLDD518 pKa = 4.41GVPRR522 pKa = 11.84ALVSHH527 pKa = 5.56YY528 pKa = 10.75QEE530 pKa = 5.13GDD532 pKa = 3.53DD533 pKa = 5.21VLVQAPKK540 pKa = 10.71RR541 pKa = 11.84LMPIISRR548 pKa = 11.84LANGTFLLGYY558 pKa = 9.39KK559 pKa = 9.73PKK561 pKa = 10.26TVVPDD566 pKa = 4.02DD567 pKa = 4.44PGHH570 pKa = 6.85AVFCGRR576 pKa = 11.84FTCMEE581 pKa = 4.49CRR583 pKa = 11.84RR584 pKa = 11.84EE585 pKa = 4.04HH586 pKa = 6.86CDD588 pKa = 3.24VNRR591 pKa = 11.84ALSKK595 pKa = 10.32FHH597 pKa = 5.47TTILPGDD604 pKa = 3.57SRR606 pKa = 11.84ALLRR610 pKa = 11.84AKK612 pKa = 10.52AMSYY616 pKa = 9.98HH617 pKa = 6.0HH618 pKa = 7.44TDD620 pKa = 1.95GHH622 pKa = 5.51TPVVGEE628 pKa = 3.88VCRR631 pKa = 11.84AILRR635 pKa = 11.84DD636 pKa = 3.54TADD639 pKa = 3.28VTDD642 pKa = 4.46AEE644 pKa = 4.68VFKK647 pKa = 10.96AMRR650 pKa = 11.84MAPGEE655 pKa = 4.13EE656 pKa = 3.69RR657 pKa = 11.84RR658 pKa = 11.84KK659 pKa = 10.4LRR661 pKa = 11.84GAVGPSRR668 pKa = 11.84ALTKK672 pKa = 10.19KK673 pKa = 10.03VRR675 pKa = 11.84PCCRR679 pKa = 11.84ASVAGTSVFDD689 pKa = 4.85IEE691 pKa = 4.61LQLAVEE697 pKa = 4.22KK698 pKa = 10.37MFRR701 pKa = 11.84RR702 pKa = 11.84DD703 pKa = 2.43GMYY706 pKa = 10.78ALRR709 pKa = 11.84TGPVIVDD716 pKa = 3.34DD717 pKa = 6.11DD718 pKa = 4.01MVEE721 pKa = 4.02DD722 pKa = 4.32HH723 pKa = 7.07SLLIYY728 pKa = 10.5RR729 pKa = 5.0

Molecular weight:
82.15 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KFJ2|A0A1L3KFJ2_9VIRU Capsid protein OS=Beihai tombus-like virus 19 OX=1922722 PE=3 SV=1
MM1 pKa = 7.78VLDD4 pKa = 4.17NLQSFARR11 pKa = 11.84RR12 pKa = 11.84SMGIAGRR19 pKa = 11.84SQADD23 pKa = 3.77FSRR26 pKa = 11.84FRR28 pKa = 11.84WTWYY32 pKa = 8.39EE33 pKa = 3.62ASGYY37 pKa = 8.0PVWSVFHH44 pKa = 6.12PLAWEE49 pKa = 3.82EE50 pKa = 4.47TITLRR55 pKa = 11.84YY56 pKa = 7.36TLLVLYY62 pKa = 9.91LSIALTLLIGSRR74 pKa = 11.84FSRR77 pKa = 11.84FAVLTTLLSVYY88 pKa = 10.69VPMFLLAQEE97 pKa = 4.84LGVTPDD103 pKa = 3.54EE104 pKa = 4.96DD105 pKa = 4.13RR106 pKa = 11.84LALTTDD112 pKa = 3.66LCEE115 pKa = 4.25DD116 pKa = 3.08WHH118 pKa = 6.23RR119 pKa = 11.84AVRR122 pKa = 11.84LWQTKK127 pKa = 9.4IGTLSRR133 pKa = 11.84RR134 pKa = 11.84QLRR137 pKa = 11.84APKK140 pKa = 9.05GCKK143 pKa = 9.44YY144 pKa = 10.57LLEE147 pKa = 5.33LGEE150 pKa = 4.24YY151 pKa = 8.57EE152 pKa = 4.95IPPPDD157 pKa = 4.26PEE159 pKa = 4.27PVQEE163 pKa = 4.05PVSWFPTFSVPRR175 pKa = 11.84WGTSTSEE182 pKa = 3.83IVKK185 pKa = 9.82PLARR189 pKa = 11.84YY190 pKa = 6.29WTVEE194 pKa = 3.63LDD196 pKa = 3.1IVQLILCLAAGWVTVTAVLHH216 pKa = 6.3LIGKK220 pKa = 7.95RR221 pKa = 11.84LRR223 pKa = 11.84RR224 pKa = 11.84SRR226 pKa = 11.84VTVTAPMWNFTHH238 pKa = 7.35RR239 pKa = 11.84DD240 pKa = 3.3VTIGLTKK247 pKa = 10.48RR248 pKa = 11.84INDD251 pKa = 3.58ARR253 pKa = 11.84NAIQPNLANGHH264 pKa = 6.54ALLAHH269 pKa = 6.47EE270 pKa = 4.93RR271 pKa = 11.84ATLEE275 pKa = 4.29KK276 pKa = 11.14GCLDD280 pKa = 3.23WLAAVSPFTRR290 pKa = 11.84DD291 pKa = 2.37IGGSLTRR298 pKa = 11.84HH299 pKa = 6.24AARR302 pKa = 11.84GPDD305 pKa = 3.16HH306 pKa = 6.74HH307 pKa = 7.94VCFPRR312 pKa = 11.84LDD314 pKa = 4.38DD315 pKa = 4.71ADD317 pKa = 3.79HH318 pKa = 6.95ARR320 pKa = 11.84WQYY323 pKa = 10.92AQGRR327 pKa = 11.84MEE329 pKa = 4.42NDD331 pKa = 2.93IGLHH335 pKa = 5.69TGQDD339 pKa = 3.79CPVSNRR345 pKa = 11.84LSMMSDD351 pKa = 2.64VDD353 pKa = 3.49YY354 pKa = 11.62HH355 pKa = 4.67MTEE358 pKa = 4.41DD359 pKa = 3.54EE360 pKa = 4.21LVRR363 pKa = 11.84AIRR366 pKa = 11.84APTLIITHH374 pKa = 6.82DD375 pKa = 4.33FVALEE380 pKa = 5.12DD381 pKa = 3.89KK382 pKa = 10.74PQTDD386 pKa = 3.18WCDD389 pKa = 3.48GEE391 pKa = 4.51ATVLVRR397 pKa = 11.84GGLVCMTVRR406 pKa = 11.84GGEE409 pKa = 4.37SYY411 pKa = 9.68VHH413 pKa = 6.83NYY415 pKa = 9.18HH416 pKa = 6.92RR417 pKa = 11.84WQNEE421 pKa = 3.62GTVATTSGAFRR432 pKa = 11.84YY433 pKa = 10.27DD434 pKa = 3.09RR435 pKa = 11.84VFTSDD440 pKa = 3.09HH441 pKa = 6.88SIVLYY446 pKa = 9.28CVPVSGDD453 pKa = 3.49YY454 pKa = 10.03RR455 pKa = 11.84VKK457 pKa = 10.21TSLGRR462 pKa = 11.84KK463 pKa = 5.94AHH465 pKa = 6.8RR466 pKa = 11.84IEE468 pKa = 4.95DD469 pKa = 3.47IKK471 pKa = 11.13VASGHH476 pKa = 5.63PLIVSRR482 pKa = 11.84GQLLVDD488 pKa = 3.71GVGYY492 pKa = 10.17DD493 pKa = 3.35VSMLMTVAFSQSTAARR509 pKa = 11.84DD510 pKa = 4.19DD511 pKa = 3.24KK512 pKa = 11.05WLANLKK518 pKa = 10.24SVLRR522 pKa = 11.84AKK524 pKa = 9.35LTAAEE529 pKa = 4.33ANNLHH534 pKa = 6.66IDD536 pKa = 3.68VVQEE540 pKa = 4.23VVVKK544 pKa = 10.66LADD547 pKa = 3.83QMALDD552 pKa = 4.57APISVITGDD561 pKa = 3.98PANLSWFQRR570 pKa = 11.84YY571 pKa = 7.23VLRR574 pKa = 11.84WLMWSGLAAPKK585 pKa = 10.35AIARR589 pKa = 11.84FLQWAVPRR597 pKa = 11.84VSDD600 pKa = 3.59WLAGRR605 pKa = 11.84SPLTARR611 pKa = 11.84MSGAWTEE618 pKa = 4.2VRR620 pKa = 11.84VPTYY624 pKa = 10.07EE625 pKa = 4.22VVWAQVQTVLTDD637 pKa = 3.32TRR639 pKa = 11.84RR640 pKa = 11.84SATQPFRR647 pKa = 11.84VPRR650 pKa = 11.84QDD652 pKa = 3.82APAALPQSDD661 pKa = 3.49NRR663 pKa = 11.84PARR666 pKa = 11.84THH668 pKa = 6.52RR669 pKa = 11.84GEE671 pKa = 3.62PDD673 pKa = 2.87RR674 pKa = 11.84RR675 pKa = 11.84DD676 pKa = 3.65RR677 pKa = 11.84EE678 pKa = 4.13EE679 pKa = 4.17GASGGAAAPSRR690 pKa = 11.84PGPVEE695 pKa = 4.02RR696 pKa = 11.84RR697 pKa = 11.84SKK699 pKa = 10.37PVSHH703 pKa = 6.68GPARR707 pKa = 11.84PAAGASGGGRR717 pKa = 11.84GGSGGSDD724 pKa = 2.79RR725 pKa = 11.84PAIQPAPGTPGASAPSGGPSVEE747 pKa = 3.88KK748 pKa = 10.37KK749 pKa = 9.54KK750 pKa = 10.52RR751 pKa = 11.84RR752 pKa = 11.84RR753 pKa = 11.84TRR755 pKa = 11.84KK756 pKa = 9.12KK757 pKa = 8.5RR758 pKa = 11.84AAAVGSEE765 pKa = 4.12GAEE768 pKa = 3.66ARR770 pKa = 11.84PAEE773 pKa = 4.32LKK775 pKa = 10.86GNPSVSKK782 pKa = 9.78PAVSQPPTGSGLLGGAPAKK801 pKa = 10.32AVAAPKK807 pKa = 9.67PPKK810 pKa = 10.5AKK812 pKa = 10.31GPKK815 pKa = 8.23KK816 pKa = 10.05QRR818 pKa = 11.84SRR820 pKa = 11.84SQSLSRR826 pKa = 11.84HH827 pKa = 4.99EE828 pKa = 5.27LGDD831 pKa = 3.54LLRR834 pKa = 11.84RR835 pKa = 11.84IAALEE840 pKa = 3.84GLGAVPTRR848 pKa = 11.84GQSGSGARR856 pKa = 11.84PKK858 pKa = 9.93QARR861 pKa = 11.84SPTPVLEE868 pKa = 4.09YY869 pKa = 10.81APRR872 pKa = 11.84SPDD875 pKa = 3.26LSEE878 pKa = 3.88QLGDD882 pKa = 3.76LSVSDD887 pKa = 5.07KK888 pKa = 10.07YY889 pKa = 9.42TAADD893 pKa = 3.84FGLDD897 pKa = 3.47VPTVSAAPSYY907 pKa = 10.65HH908 pKa = 6.66AVPPPAVYY916 pKa = 10.23HH917 pKa = 6.26PGGAGGAGPTRR928 pKa = 11.84PPHH931 pKa = 5.2PTPRR935 pKa = 11.84TQGRR939 pKa = 11.84GQRR942 pKa = 11.84PARR945 pKa = 11.84VQSAVRR951 pKa = 11.84GGPGGQRR958 pKa = 11.84AQAAGARR965 pKa = 11.84GLPVPYY971 pKa = 9.75KK972 pKa = 10.92VKK974 pKa = 9.66TAA976 pKa = 3.62

Molecular weight:
106.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2126

421

976

708.7

78.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.831 ± 0.919

1.552 ± 0.497

5.927 ± 0.444

4.468 ± 0.887

2.775 ± 0.398

7.432 ± 0.875

2.54 ± 0.265

3.01 ± 0.219

3.575 ± 0.269

9.643 ± 0.987

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.834 ± 0.225

2.07 ± 0.415

7.385 ± 0.555

3.716 ± 0.605

8.984 ± 1.102

7.291 ± 0.434

6.35 ± 0.581

7.291 ± 0.556

2.117 ± 0.458

2.211 ± 0.182

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski