Beihai tombus-like virus 19
Average proteome isoelectric point is 8.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFJ6|A0A1L3KFJ6_9VIRU RNA-directed RNA polymerase OS=Beihai tombus-like virus 19 OX=1922722 PE=4 SV=1
MM1 pKa = 7.81 LLPPFLSRR9 pKa = 11.84 TIALPARR16 pKa = 11.84 TVEE19 pKa = 3.98 NQIAGIEE26 pKa = 4.06 KK27 pKa = 9.85 RR28 pKa = 11.84 VLAVVPQPHH37 pKa = 7.61 PDD39 pKa = 3.43 LDD41 pKa = 3.61 PWNDD45 pKa = 3.19 EE46 pKa = 3.67 ASPYY50 pKa = 8.93 HH51 pKa = 6.3 TDD53 pKa = 3.18 RR54 pKa = 11.84 LALPRR59 pKa = 11.84 EE60 pKa = 3.88 RR61 pKa = 11.84 LGVVEE66 pKa = 5.62 EE67 pKa = 4.46 DD68 pKa = 5.09 LEE70 pKa = 4.62 DD71 pKa = 3.64 LTALLSSQRR80 pKa = 11.84 QEE82 pKa = 3.68 HH83 pKa = 6.4 RR84 pKa = 11.84 EE85 pKa = 3.66 RR86 pKa = 11.84 ALRR89 pKa = 11.84 LVDD92 pKa = 3.75 LAWRR96 pKa = 11.84 RR97 pKa = 11.84 RR98 pKa = 11.84 RR99 pKa = 11.84 GAEE102 pKa = 3.87 LARR105 pKa = 11.84 SEE107 pKa = 4.26 LPPWEE112 pKa = 4.27 ARR114 pKa = 11.84 GQRR117 pKa = 11.84 RR118 pKa = 11.84 GRR120 pKa = 11.84 LNSRR124 pKa = 11.84 EE125 pKa = 3.92 ILVSANLPCLSRR137 pKa = 11.84 LRR139 pKa = 11.84 DD140 pKa = 3.56 QDD142 pKa = 3.79 CLEE145 pKa = 3.86 VHH147 pKa = 6.23 QRR149 pKa = 11.84 KK150 pKa = 8.36 LWLLPSLLRR159 pKa = 11.84 LRR161 pKa = 11.84 DD162 pKa = 3.65 PRR164 pKa = 11.84 SSGVAVRR171 pKa = 11.84 VFPAMSWEE179 pKa = 4.4 TFCDD183 pKa = 3.49 EE184 pKa = 4.85 SPRR187 pKa = 11.84 LRR189 pKa = 11.84 DD190 pKa = 3.43 WVPSRR195 pKa = 11.84 LGDD198 pKa = 3.83 SQDD201 pKa = 3.6 PEE203 pKa = 4.4 HH204 pKa = 6.75 ALSRR208 pKa = 11.84 PAHH211 pKa = 6.3 RR212 pKa = 11.84 PLFSNTPHH220 pKa = 6.87 ARR222 pKa = 11.84 QICRR226 pKa = 11.84 NSWGTCPCLTSTQLLTSVSTYY247 pKa = 9.84 RR248 pKa = 11.84 QCLLPLPTTLCRR260 pKa = 11.84 PPPSTTLVEE269 pKa = 4.15 PVVLVLPVHH278 pKa = 7.14 LIRR281 pKa = 11.84 HH282 pKa = 6.62 LGPKK286 pKa = 10.0 DD287 pKa = 3.54 VGSDD291 pKa = 3.38 LPEE294 pKa = 4.09 FSPRR298 pKa = 11.84 FAEE301 pKa = 4.34 DD302 pKa = 3.84 LEE304 pKa = 4.82 DD305 pKa = 3.82 SGRR308 pKa = 11.84 KK309 pKa = 9.12 LLEE312 pKa = 4.53 HH313 pKa = 7.36 EE314 pKa = 5.0 DD315 pKa = 3.81 FQFLTRR321 pKa = 11.84 SKK323 pKa = 10.46 PLEE326 pKa = 3.98 FEE328 pKa = 4.65 DD329 pKa = 3.49 WVKK332 pKa = 10.6 RR333 pKa = 11.84 YY334 pKa = 8.59 PPHH337 pKa = 6.61 RR338 pKa = 11.84 RR339 pKa = 11.84 EE340 pKa = 4.06 EE341 pKa = 4.0 LRR343 pKa = 11.84 EE344 pKa = 3.99 ARR346 pKa = 11.84 TKK348 pKa = 10.88 ALRR351 pKa = 11.84 NGISPKK357 pKa = 10.34 DD358 pKa = 3.57 ANLKK362 pKa = 9.98 VFMKK366 pKa = 10.42 IEE368 pKa = 4.18 GTTTGTDD375 pKa = 3.28 PRR377 pKa = 11.84 NISPRR382 pKa = 11.84 TDD384 pKa = 2.8 AFLSIMGPYY393 pKa = 9.02 VAGIEE398 pKa = 4.15 HH399 pKa = 7.14 AAVQEE404 pKa = 4.14 PRR406 pKa = 11.84 CPFLVKK412 pKa = 10.64 GMDD415 pKa = 2.98 VSTRR419 pKa = 11.84 GSYY422 pKa = 10.86 LSRR425 pKa = 11.84 TDD427 pKa = 2.77 KK428 pKa = 10.91 GARR431 pKa = 11.84 AEE433 pKa = 4.09 IDD435 pKa = 3.56 FSRR438 pKa = 11.84 FDD440 pKa = 3.44 QSLSTAVLQAFEE452 pKa = 4.16 LAAFRR457 pKa = 11.84 VAYY460 pKa = 9.66 PEE462 pKa = 4.35 GLHH465 pKa = 6.73 PLLDD469 pKa = 3.78 EE470 pKa = 5.2 CLACLDD476 pKa = 3.85 EE477 pKa = 4.74 MKK479 pKa = 10.68 GFSDD483 pKa = 3.8 LGVSYY488 pKa = 10.34 RR489 pKa = 11.84 VEE491 pKa = 3.94 ATRR494 pKa = 11.84 ASGDD498 pKa = 3.04 AHH500 pKa = 5.49 TSIMNGVDD508 pKa = 3.69 NRR510 pKa = 11.84 CLCQYY515 pKa = 10.8 VLDD518 pKa = 4.41 GVPRR522 pKa = 11.84 ALVSHH527 pKa = 5.56 YY528 pKa = 10.75 QEE530 pKa = 5.13 GDD532 pKa = 3.53 DD533 pKa = 5.21 VLVQAPKK540 pKa = 10.71 RR541 pKa = 11.84 LMPIISRR548 pKa = 11.84 LANGTFLLGYY558 pKa = 9.39 KK559 pKa = 9.73 PKK561 pKa = 10.26 TVVPDD566 pKa = 4.02 DD567 pKa = 4.44 PGHH570 pKa = 6.85 AVFCGRR576 pKa = 11.84 FTCMEE581 pKa = 4.49 CRR583 pKa = 11.84 RR584 pKa = 11.84 EE585 pKa = 4.04 HH586 pKa = 6.86 CDD588 pKa = 3.24 VNRR591 pKa = 11.84 ALSKK595 pKa = 10.32 FHH597 pKa = 5.47 TTILPGDD604 pKa = 3.57 SRR606 pKa = 11.84 ALLRR610 pKa = 11.84 AKK612 pKa = 10.52 AMSYY616 pKa = 9.98 HH617 pKa = 6.0 HH618 pKa = 7.44 TDD620 pKa = 1.95 GHH622 pKa = 5.51 TPVVGEE628 pKa = 3.88 VCRR631 pKa = 11.84 AILRR635 pKa = 11.84 DD636 pKa = 3.54 TADD639 pKa = 3.28 VTDD642 pKa = 4.46 AEE644 pKa = 4.68 VFKK647 pKa = 10.96 AMRR650 pKa = 11.84 MAPGEE655 pKa = 4.13 EE656 pKa = 3.69 RR657 pKa = 11.84 RR658 pKa = 11.84 KK659 pKa = 10.4 LRR661 pKa = 11.84 GAVGPSRR668 pKa = 11.84 ALTKK672 pKa = 10.19 KK673 pKa = 10.03 VRR675 pKa = 11.84 PCCRR679 pKa = 11.84 ASVAGTSVFDD689 pKa = 4.85 IEE691 pKa = 4.61 LQLAVEE697 pKa = 4.22 KK698 pKa = 10.37 MFRR701 pKa = 11.84 RR702 pKa = 11.84 DD703 pKa = 2.43 GMYY706 pKa = 10.78 ALRR709 pKa = 11.84 TGPVIVDD716 pKa = 3.34 DD717 pKa = 6.11 DD718 pKa = 4.01 MVEE721 pKa = 4.02 DD722 pKa = 4.32 HH723 pKa = 7.07 SLLIYY728 pKa = 10.5 RR729 pKa = 5.0
Molecular weight: 82.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.097
IPC2_protein 7.41
IPC_protein 7.22
Toseland 6.927
ProMoST 7.907
Dawson 7.907
Bjellqvist 8.419
Wikipedia 7.819
Rodwell 7.936
Grimsley 7.0
Solomon 7.936
Lehninger 7.966
Nozaki 8.741
DTASelect 8.083
Thurlkill 8.141
EMBOSS 8.141
Sillero 8.507
Patrickios 4.609
IPC_peptide 7.936
IPC2_peptide 7.717
IPC2.peptide.svr19 7.434
Protein with the highest isoelectric point:
>tr|A0A1L3KFJ2|A0A1L3KFJ2_9VIRU Capsid protein OS=Beihai tombus-like virus 19 OX=1922722 PE=3 SV=1
MM1 pKa = 7.78 VLDD4 pKa = 4.17 NLQSFARR11 pKa = 11.84 RR12 pKa = 11.84 SMGIAGRR19 pKa = 11.84 SQADD23 pKa = 3.77 FSRR26 pKa = 11.84 FRR28 pKa = 11.84 WTWYY32 pKa = 8.39 EE33 pKa = 3.62 ASGYY37 pKa = 8.0 PVWSVFHH44 pKa = 6.12 PLAWEE49 pKa = 3.82 EE50 pKa = 4.47 TITLRR55 pKa = 11.84 YY56 pKa = 7.36 TLLVLYY62 pKa = 9.91 LSIALTLLIGSRR74 pKa = 11.84 FSRR77 pKa = 11.84 FAVLTTLLSVYY88 pKa = 10.69 VPMFLLAQEE97 pKa = 4.84 LGVTPDD103 pKa = 3.54 EE104 pKa = 4.96 DD105 pKa = 4.13 RR106 pKa = 11.84 LALTTDD112 pKa = 3.66 LCEE115 pKa = 4.25 DD116 pKa = 3.08 WHH118 pKa = 6.23 RR119 pKa = 11.84 AVRR122 pKa = 11.84 LWQTKK127 pKa = 9.4 IGTLSRR133 pKa = 11.84 RR134 pKa = 11.84 QLRR137 pKa = 11.84 APKK140 pKa = 9.05 GCKK143 pKa = 9.44 YY144 pKa = 10.57 LLEE147 pKa = 5.33 LGEE150 pKa = 4.24 YY151 pKa = 8.57 EE152 pKa = 4.95 IPPPDD157 pKa = 4.26 PEE159 pKa = 4.27 PVQEE163 pKa = 4.05 PVSWFPTFSVPRR175 pKa = 11.84 WGTSTSEE182 pKa = 3.83 IVKK185 pKa = 9.82 PLARR189 pKa = 11.84 YY190 pKa = 6.29 WTVEE194 pKa = 3.63 LDD196 pKa = 3.1 IVQLILCLAAGWVTVTAVLHH216 pKa = 6.3 LIGKK220 pKa = 7.95 RR221 pKa = 11.84 LRR223 pKa = 11.84 RR224 pKa = 11.84 SRR226 pKa = 11.84 VTVTAPMWNFTHH238 pKa = 7.35 RR239 pKa = 11.84 DD240 pKa = 3.3 VTIGLTKK247 pKa = 10.48 RR248 pKa = 11.84 INDD251 pKa = 3.58 ARR253 pKa = 11.84 NAIQPNLANGHH264 pKa = 6.54 ALLAHH269 pKa = 6.47 EE270 pKa = 4.93 RR271 pKa = 11.84 ATLEE275 pKa = 4.29 KK276 pKa = 11.14 GCLDD280 pKa = 3.23 WLAAVSPFTRR290 pKa = 11.84 DD291 pKa = 2.37 IGGSLTRR298 pKa = 11.84 HH299 pKa = 6.24 AARR302 pKa = 11.84 GPDD305 pKa = 3.16 HH306 pKa = 6.74 HH307 pKa = 7.94 VCFPRR312 pKa = 11.84 LDD314 pKa = 4.38 DD315 pKa = 4.71 ADD317 pKa = 3.79 HH318 pKa = 6.95 ARR320 pKa = 11.84 WQYY323 pKa = 10.92 AQGRR327 pKa = 11.84 MEE329 pKa = 4.42 NDD331 pKa = 2.93 IGLHH335 pKa = 5.69 TGQDD339 pKa = 3.79 CPVSNRR345 pKa = 11.84 LSMMSDD351 pKa = 2.64 VDD353 pKa = 3.49 YY354 pKa = 11.62 HH355 pKa = 4.67 MTEE358 pKa = 4.41 DD359 pKa = 3.54 EE360 pKa = 4.21 LVRR363 pKa = 11.84 AIRR366 pKa = 11.84 APTLIITHH374 pKa = 6.82 DD375 pKa = 4.33 FVALEE380 pKa = 5.12 DD381 pKa = 3.89 KK382 pKa = 10.74 PQTDD386 pKa = 3.18 WCDD389 pKa = 3.48 GEE391 pKa = 4.51 ATVLVRR397 pKa = 11.84 GGLVCMTVRR406 pKa = 11.84 GGEE409 pKa = 4.37 SYY411 pKa = 9.68 VHH413 pKa = 6.83 NYY415 pKa = 9.18 HH416 pKa = 6.92 RR417 pKa = 11.84 WQNEE421 pKa = 3.62 GTVATTSGAFRR432 pKa = 11.84 YY433 pKa = 10.27 DD434 pKa = 3.09 RR435 pKa = 11.84 VFTSDD440 pKa = 3.09 HH441 pKa = 6.88 SIVLYY446 pKa = 9.28 CVPVSGDD453 pKa = 3.49 YY454 pKa = 10.03 RR455 pKa = 11.84 VKK457 pKa = 10.21 TSLGRR462 pKa = 11.84 KK463 pKa = 5.94 AHH465 pKa = 6.8 RR466 pKa = 11.84 IEE468 pKa = 4.95 DD469 pKa = 3.47 IKK471 pKa = 11.13 VASGHH476 pKa = 5.63 PLIVSRR482 pKa = 11.84 GQLLVDD488 pKa = 3.71 GVGYY492 pKa = 10.17 DD493 pKa = 3.35 VSMLMTVAFSQSTAARR509 pKa = 11.84 DD510 pKa = 4.19 DD511 pKa = 3.24 KK512 pKa = 11.05 WLANLKK518 pKa = 10.24 SVLRR522 pKa = 11.84 AKK524 pKa = 9.35 LTAAEE529 pKa = 4.33 ANNLHH534 pKa = 6.66 IDD536 pKa = 3.68 VVQEE540 pKa = 4.23 VVVKK544 pKa = 10.66 LADD547 pKa = 3.83 QMALDD552 pKa = 4.57 APISVITGDD561 pKa = 3.98 PANLSWFQRR570 pKa = 11.84 YY571 pKa = 7.23 VLRR574 pKa = 11.84 WLMWSGLAAPKK585 pKa = 10.35 AIARR589 pKa = 11.84 FLQWAVPRR597 pKa = 11.84 VSDD600 pKa = 3.59 WLAGRR605 pKa = 11.84 SPLTARR611 pKa = 11.84 MSGAWTEE618 pKa = 4.2 VRR620 pKa = 11.84 VPTYY624 pKa = 10.07 EE625 pKa = 4.22 VVWAQVQTVLTDD637 pKa = 3.32 TRR639 pKa = 11.84 RR640 pKa = 11.84 SATQPFRR647 pKa = 11.84 VPRR650 pKa = 11.84 QDD652 pKa = 3.82 APAALPQSDD661 pKa = 3.49 NRR663 pKa = 11.84 PARR666 pKa = 11.84 THH668 pKa = 6.52 RR669 pKa = 11.84 GEE671 pKa = 3.62 PDD673 pKa = 2.87 RR674 pKa = 11.84 RR675 pKa = 11.84 DD676 pKa = 3.65 RR677 pKa = 11.84 EE678 pKa = 4.13 EE679 pKa = 4.17 GASGGAAAPSRR690 pKa = 11.84 PGPVEE695 pKa = 4.02 RR696 pKa = 11.84 RR697 pKa = 11.84 SKK699 pKa = 10.37 PVSHH703 pKa = 6.68 GPARR707 pKa = 11.84 PAAGASGGGRR717 pKa = 11.84 GGSGGSDD724 pKa = 2.79 RR725 pKa = 11.84 PAIQPAPGTPGASAPSGGPSVEE747 pKa = 3.88 KK748 pKa = 10.37 KK749 pKa = 9.54 KK750 pKa = 10.52 RR751 pKa = 11.84 RR752 pKa = 11.84 RR753 pKa = 11.84 TRR755 pKa = 11.84 KK756 pKa = 9.12 KK757 pKa = 8.5 RR758 pKa = 11.84 AAAVGSEE765 pKa = 4.12 GAEE768 pKa = 3.66 ARR770 pKa = 11.84 PAEE773 pKa = 4.32 LKK775 pKa = 10.86 GNPSVSKK782 pKa = 9.78 PAVSQPPTGSGLLGGAPAKK801 pKa = 10.32 AVAAPKK807 pKa = 9.67 PPKK810 pKa = 10.5 AKK812 pKa = 10.31 GPKK815 pKa = 8.23 KK816 pKa = 10.05 QRR818 pKa = 11.84 SRR820 pKa = 11.84 SQSLSRR826 pKa = 11.84 HH827 pKa = 4.99 EE828 pKa = 5.27 LGDD831 pKa = 3.54 LLRR834 pKa = 11.84 RR835 pKa = 11.84 IAALEE840 pKa = 3.84 GLGAVPTRR848 pKa = 11.84 GQSGSGARR856 pKa = 11.84 PKK858 pKa = 9.93 QARR861 pKa = 11.84 SPTPVLEE868 pKa = 4.09 YY869 pKa = 10.81 APRR872 pKa = 11.84 SPDD875 pKa = 3.26 LSEE878 pKa = 3.88 QLGDD882 pKa = 3.76 LSVSDD887 pKa = 5.07 KK888 pKa = 10.07 YY889 pKa = 9.42 TAADD893 pKa = 3.84 FGLDD897 pKa = 3.47 VPTVSAAPSYY907 pKa = 10.65 HH908 pKa = 6.66 AVPPPAVYY916 pKa = 10.23 HH917 pKa = 6.26 PGGAGGAGPTRR928 pKa = 11.84 PPHH931 pKa = 5.2 PTPRR935 pKa = 11.84 TQGRR939 pKa = 11.84 GQRR942 pKa = 11.84 PARR945 pKa = 11.84 VQSAVRR951 pKa = 11.84 GGPGGQRR958 pKa = 11.84 AQAAGARR965 pKa = 11.84 GLPVPYY971 pKa = 9.75 KK972 pKa = 10.92 VKK974 pKa = 9.66 TAA976 pKa = 3.62
Molecular weight: 106.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.238
IPC2_protein 9.238
IPC_protein 9.443
Toseland 9.955
ProMoST 9.721
Dawson 10.189
Bjellqvist 9.897
Wikipedia 10.379
Rodwell 10.409
Grimsley 10.262
Solomon 10.218
Lehninger 10.175
Nozaki 9.999
DTASelect 9.882
Thurlkill 10.043
EMBOSS 10.379
Sillero 10.116
Patrickios 9.589
IPC_peptide 10.218
IPC2_peptide 8.712
IPC2.peptide.svr19 8.226
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2126
421
976
708.7
78.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.831 ± 0.919
1.552 ± 0.497
5.927 ± 0.444
4.468 ± 0.887
2.775 ± 0.398
7.432 ± 0.875
2.54 ± 0.265
3.01 ± 0.219
3.575 ± 0.269
9.643 ± 0.987
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.834 ± 0.225
2.07 ± 0.415
7.385 ± 0.555
3.716 ± 0.605
8.984 ± 1.102
7.291 ± 0.434
6.35 ± 0.581
7.291 ± 0.556
2.117 ± 0.458
2.211 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here